In vivo and in vitro genotoxic effects of zerumbone.

Zerumbone (ZER) is derived from Zingiber zerumbet smith from the Zingiberaceae family. It has been shown to have anti-cancer and apoptosis-inducing properties against various human tumour cells. The aim of our study was to assess the genotoxic effects of ZER in cultured human peripheral blood lympho...

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Main Authors: Al-Zubairi, Adel Sharaf, Abdul, Ahmad Bustamam, Yousif, Mohammed, Abdelwahab, Siddig Ibrahim, Elhassan Taha, Manal Mohamed, Mohan, Syam Murali
Format: Article
Language:English
English
Published: Taylor & Francis 2010
Online Access:http://psasir.upm.edu.my/id/eprint/15174/
http://psasir.upm.edu.my/id/eprint/15174/1/In%20vivo%20and%20in%20vitro%20genotoxic%20effects%20of%20zerumbone.pdf
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author Al-Zubairi, Adel Sharaf
Abdul, Ahmad Bustamam
Yousif, Mohammed
Abdelwahab, Siddig Ibrahim
Elhassan Taha, Manal Mohamed
Mohan, Syam Murali
author_facet Al-Zubairi, Adel Sharaf
Abdul, Ahmad Bustamam
Yousif, Mohammed
Abdelwahab, Siddig Ibrahim
Elhassan Taha, Manal Mohamed
Mohan, Syam Murali
author_sort Al-Zubairi, Adel Sharaf
building UPM Institutional Repository
collection Online Access
description Zerumbone (ZER) is derived from Zingiber zerumbet smith from the Zingiberaceae family. It has been shown to have anti-cancer and apoptosis-inducing properties against various human tumour cells. The aim of our study was to assess the genotoxic effects of ZER in cultured human peripheral blood lymphocytes, Chinese Hamster Ovary (CHO) cells and rat bone marrow polychromatic erythrocytes (PCEs) using micronucleus test (MN). All in vitro treatments were carried out in the absence of any exogenous metabolic activation system. Mitomycin C (MMC) was used as a positive control for in vitro treatments, while cisplatin was used as a positive micronucleus inducer in rat bone marrow PCEs. ZER at high concentrations induced an apparent signifi cant increase in the frequency of micronuclei in vivo (1000 mg/kg b.w) and in vitro (40 and 80 μM) compared to concurrent control values. Our in vivo and in vitro cytogenotoxicity studies suggest that high doses of ZER may be genotoxic and cytotoxic.
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spelling upm-151742015-11-02T03:38:48Z http://psasir.upm.edu.my/id/eprint/15174/ In vivo and in vitro genotoxic effects of zerumbone. Al-Zubairi, Adel Sharaf Abdul, Ahmad Bustamam Yousif, Mohammed Abdelwahab, Siddig Ibrahim Elhassan Taha, Manal Mohamed Mohan, Syam Murali Zerumbone (ZER) is derived from Zingiber zerumbet smith from the Zingiberaceae family. It has been shown to have anti-cancer and apoptosis-inducing properties against various human tumour cells. The aim of our study was to assess the genotoxic effects of ZER in cultured human peripheral blood lymphocytes, Chinese Hamster Ovary (CHO) cells and rat bone marrow polychromatic erythrocytes (PCEs) using micronucleus test (MN). All in vitro treatments were carried out in the absence of any exogenous metabolic activation system. Mitomycin C (MMC) was used as a positive control for in vitro treatments, while cisplatin was used as a positive micronucleus inducer in rat bone marrow PCEs. ZER at high concentrations induced an apparent signifi cant increase in the frequency of micronuclei in vivo (1000 mg/kg b.w) and in vitro (40 and 80 μM) compared to concurrent control values. Our in vivo and in vitro cytogenotoxicity studies suggest that high doses of ZER may be genotoxic and cytotoxic. Taylor & Francis 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15174/1/In%20vivo%20and%20in%20vitro%20genotoxic%20effects%20of%20zerumbone.pdf Al-Zubairi, Adel Sharaf and Abdul, Ahmad Bustamam and Yousif, Mohammed and Abdelwahab, Siddig Ibrahim and Elhassan Taha, Manal Mohamed and Mohan, Syam Murali (2010) In vivo and in vitro genotoxic effects of zerumbone. Caryologia, 63 (1). pp. 11-17. ISSN 0008-7114, ESSN: 2165-5391 http://www.tandf.co.uk English
spellingShingle Al-Zubairi, Adel Sharaf
Abdul, Ahmad Bustamam
Yousif, Mohammed
Abdelwahab, Siddig Ibrahim
Elhassan Taha, Manal Mohamed
Mohan, Syam Murali
In vivo and in vitro genotoxic effects of zerumbone.
title In vivo and in vitro genotoxic effects of zerumbone.
title_full In vivo and in vitro genotoxic effects of zerumbone.
title_fullStr In vivo and in vitro genotoxic effects of zerumbone.
title_full_unstemmed In vivo and in vitro genotoxic effects of zerumbone.
title_short In vivo and in vitro genotoxic effects of zerumbone.
title_sort in vivo and in vitro genotoxic effects of zerumbone.
url http://psasir.upm.edu.my/id/eprint/15174/
http://psasir.upm.edu.my/id/eprint/15174/
http://psasir.upm.edu.my/id/eprint/15174/1/In%20vivo%20and%20in%20vitro%20genotoxic%20effects%20of%20zerumbone.pdf