In vitro anticoagulant activities of Melastoma malabathricum Linn. aqueous leaf extract : a preliminary novel finding.

Limitations of existing anticoagulants have prompted a search for novel agents of natural origin.Fundamentals to this research was the observation that the aqueous leaf extract of Melastoma malabathricum Linn. possesses potent anticoagulant property.In vitro coagulation assays such as activated par...

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Main Authors: Manicam, Caroline, Abdullah, Janna Ong, Mohd Tohit, Eusni Rahayu, Seman, Zainina, Chin, Sieo Chin, Hamid, Muhajir
Format: Article
Language:English
English
Published: Academic Journals 2010
Online Access:http://psasir.upm.edu.my/id/eprint/15156/
http://psasir.upm.edu.my/id/eprint/15156/1/In%20vitro%20anticoagulant%20activities%20of%20Melastoma%20malabathricum%20Linn.pdf
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author Manicam, Caroline
Abdullah, Janna Ong
Mohd Tohit, Eusni Rahayu
Seman, Zainina
Chin, Sieo Chin
Hamid, Muhajir
author_facet Manicam, Caroline
Abdullah, Janna Ong
Mohd Tohit, Eusni Rahayu
Seman, Zainina
Chin, Sieo Chin
Hamid, Muhajir
author_sort Manicam, Caroline
building UPM Institutional Repository
collection Online Access
description Limitations of existing anticoagulants have prompted a search for novel agents of natural origin.Fundamentals to this research was the observation that the aqueous leaf extract of Melastoma malabathricum Linn. possesses potent anticoagulant property.In vitro coagulation assays such as activated partial thromboplastin time (aPTT), prothrombin time (PT) thrombin time (TT) and mixing studies were performed on citrated plasmas of healthy volunteer donors spiked with different concentrations of the leaf exact (100-1000μg/ml).The results showed that aPTT of plasma samples spiked with extract was markedly prolonged in a concentration-dependent manner (p<0.001), but was otherwise for PT and TT. Both types of mixing studies corrected the initially prolonged aPTT to normal range.The extract exhibited no inter-gender variability in its anticoagulant activity.This study highlights that the anticoagulant activity of M. malabathricumaqueous leaf extract affects the intrinsic pathway of the coagulation cascade by causing clotting factor(s) deficiency.
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publisher Academic Journals
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spelling upm-151562015-10-21T03:36:20Z http://psasir.upm.edu.my/id/eprint/15156/ In vitro anticoagulant activities of Melastoma malabathricum Linn. aqueous leaf extract : a preliminary novel finding. Manicam, Caroline Abdullah, Janna Ong Mohd Tohit, Eusni Rahayu Seman, Zainina Chin, Sieo Chin Hamid, Muhajir Limitations of existing anticoagulants have prompted a search for novel agents of natural origin.Fundamentals to this research was the observation that the aqueous leaf extract of Melastoma malabathricum Linn. possesses potent anticoagulant property.In vitro coagulation assays such as activated partial thromboplastin time (aPTT), prothrombin time (PT) thrombin time (TT) and mixing studies were performed on citrated plasmas of healthy volunteer donors spiked with different concentrations of the leaf exact (100-1000μg/ml).The results showed that aPTT of plasma samples spiked with extract was markedly prolonged in a concentration-dependent manner (p<0.001), but was otherwise for PT and TT. Both types of mixing studies corrected the initially prolonged aPTT to normal range.The extract exhibited no inter-gender variability in its anticoagulant activity.This study highlights that the anticoagulant activity of M. malabathricumaqueous leaf extract affects the intrinsic pathway of the coagulation cascade by causing clotting factor(s) deficiency. Academic Journals 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15156/1/In%20vitro%20anticoagulant%20activities%20of%20Melastoma%20malabathricum%20Linn.pdf Manicam, Caroline and Abdullah, Janna Ong and Mohd Tohit, Eusni Rahayu and Seman, Zainina and Chin, Sieo Chin and Hamid, Muhajir (2010) In vitro anticoagulant activities of Melastoma malabathricum Linn. aqueous leaf extract : a preliminary novel finding. Journal of Medicinal Plants Research, 4 (14). pp. 1464-1472. ISSN 1996-0875 http://www.academicjournals.org/JMPR 10.5897/JMPR10.136 English
spellingShingle Manicam, Caroline
Abdullah, Janna Ong
Mohd Tohit, Eusni Rahayu
Seman, Zainina
Chin, Sieo Chin
Hamid, Muhajir
In vitro anticoagulant activities of Melastoma malabathricum Linn. aqueous leaf extract : a preliminary novel finding.
title In vitro anticoagulant activities of Melastoma malabathricum Linn. aqueous leaf extract : a preliminary novel finding.
title_full In vitro anticoagulant activities of Melastoma malabathricum Linn. aqueous leaf extract : a preliminary novel finding.
title_fullStr In vitro anticoagulant activities of Melastoma malabathricum Linn. aqueous leaf extract : a preliminary novel finding.
title_full_unstemmed In vitro anticoagulant activities of Melastoma malabathricum Linn. aqueous leaf extract : a preliminary novel finding.
title_short In vitro anticoagulant activities of Melastoma malabathricum Linn. aqueous leaf extract : a preliminary novel finding.
title_sort in vitro anticoagulant activities of melastoma malabathricum linn. aqueous leaf extract : a preliminary novel finding.
url http://psasir.upm.edu.my/id/eprint/15156/
http://psasir.upm.edu.my/id/eprint/15156/
http://psasir.upm.edu.my/id/eprint/15156/
http://psasir.upm.edu.my/id/eprint/15156/1/In%20vitro%20anticoagulant%20activities%20of%20Melastoma%20malabathricum%20Linn.pdf