Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology
Lactic acid bacteria (Lactococcus lactis NZ9000,Lactococcus lactis MG1363, Lactobacillus plantarum I-UL4 and Lactobacillus johnsonii DSM 20553) have been screened for their ability to produce folate intracellularly and/or extracellularly. L. plantarum I-UL4 was shown to be superior producer of folat...
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| Format: | Article |
| Language: | English |
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Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15142/ http://psasir.upm.edu.my/id/eprint/15142/1/Improvement%20of%20folate%20biosynthesis%20by%20lactic%20acid%20bacteria%20using%20response%20surface%20methodology.pdf |
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| author | Muhamad Nor, Norfarina Mohamad, Rosfarizan Foo, Hooi Ling Abdul Rahim, Raha |
| author_facet | Muhamad Nor, Norfarina Mohamad, Rosfarizan Foo, Hooi Ling Abdul Rahim, Raha |
| author_sort | Muhamad Nor, Norfarina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Lactic acid bacteria (Lactococcus lactis NZ9000,Lactococcus lactis MG1363, Lactobacillus plantarum I-UL4 and Lactobacillus johnsonii DSM 20553) have been screened for their ability to produce folate intracellularly and/or extracellularly. L. plantarum I-UL4 was shown to be superior producer of folate compared to other strains. Statistically based experimental designs were used to optimize the medium formulation for the growth of L. plantarum I-UL4 and folate biosynthesis. The optimal values of important factors were determined by response surface methodology (RSM). The effects of carbon sources, nitrogen sources and para-aminobenzoic acid (PABA) concentrations on folate biosynthesis were determined prior to RSM study. The biosynthesis of folate by L. plantarum I-UL4 increased from 36.36 to 60.39 mg/L using the optimized medium formulation compared to the selective Man de Rogosa Sharpe (MRS) medium. Conditions for the optimal growth of L. plantarum I-UL4 and folate biosynthesis as suggested by RSM were as follows: lactose 20 g/L, meat extract 16.57 g/L and PABA 10 mM. |
| first_indexed | 2025-11-15T08:01:37Z |
| format | Article |
| id | upm-15142 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:01:37Z |
| publishDate | 2010 |
| publisher | Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-151422017-08-14T03:35:11Z http://psasir.upm.edu.my/id/eprint/15142/ Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology Muhamad Nor, Norfarina Mohamad, Rosfarizan Foo, Hooi Ling Abdul Rahim, Raha Lactic acid bacteria (Lactococcus lactis NZ9000,Lactococcus lactis MG1363, Lactobacillus plantarum I-UL4 and Lactobacillus johnsonii DSM 20553) have been screened for their ability to produce folate intracellularly and/or extracellularly. L. plantarum I-UL4 was shown to be superior producer of folate compared to other strains. Statistically based experimental designs were used to optimize the medium formulation for the growth of L. plantarum I-UL4 and folate biosynthesis. The optimal values of important factors were determined by response surface methodology (RSM). The effects of carbon sources, nitrogen sources and para-aminobenzoic acid (PABA) concentrations on folate biosynthesis were determined prior to RSM study. The biosynthesis of folate by L. plantarum I-UL4 increased from 36.36 to 60.39 mg/L using the optimized medium formulation compared to the selective Man de Rogosa Sharpe (MRS) medium. Conditions for the optimal growth of L. plantarum I-UL4 and folate biosynthesis as suggested by RSM were as follows: lactose 20 g/L, meat extract 16.57 g/L and PABA 10 mM. Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15142/1/Improvement%20of%20folate%20biosynthesis%20by%20lactic%20acid%20bacteria%20using%20response%20surface%20methodology.pdf Muhamad Nor, Norfarina and Mohamad, Rosfarizan and Foo, Hooi Ling and Abdul Rahim, Raha (2010) Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology. Food Technology and Biotechnology, 48 (2). pp. 243-250. ISSN 1330-9862; ESSN: 1334-2606 http://www.ftb.com.hr/index.php/archives/61-volume-48-issue-no-2/165 |
| spellingShingle | Muhamad Nor, Norfarina Mohamad, Rosfarizan Foo, Hooi Ling Abdul Rahim, Raha Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology |
| title | Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology |
| title_full | Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology |
| title_fullStr | Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology |
| title_full_unstemmed | Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology |
| title_short | Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology |
| title_sort | improvement of folate biosynthesis by lactic acid bacteria using response surface methodology |
| url | http://psasir.upm.edu.my/id/eprint/15142/ http://psasir.upm.edu.my/id/eprint/15142/ http://psasir.upm.edu.my/id/eprint/15142/1/Improvement%20of%20folate%20biosynthesis%20by%20lactic%20acid%20bacteria%20using%20response%20surface%20methodology.pdf |