Immunohistochemical detection of phospho-Akt, phospho-BAD, HER2 and oestrogen receptors α and β in Malaysian breast cancer patients
Activation of Akt signaling pathway has been documented in various human malignancies, including breast carcinoma. The objective of this study is to determine the incidence of Akt phosphorylation in breast tumours and its relationship with expression of ER-α, ER-β, HER2, Ki-67 and phosphorylated Bcl...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15099/ http://psasir.upm.edu.my/id/eprint/15099/1/Immunohistochemical%20detection%20of%20phospho-Akt%2C%20phospho-BAD%2C%20HER2%20and%20oestrogen%20receptors%20%CE%B1%20and%20%CE%B2%20in%20Malaysian%20breast%20cancer%20patients.pdf |
| _version_ | 1848842582115745792 |
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| author | Seow, Heng Fong Yip, Wai Kien Loh, Hui Woon Ithnin, Hairuszah Por, Patricia Muhammad, Rohaizak |
| author_facet | Seow, Heng Fong Yip, Wai Kien Loh, Hui Woon Ithnin, Hairuszah Por, Patricia Muhammad, Rohaizak |
| author_sort | Seow, Heng Fong |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Activation of Akt signaling pathway has been documented in various human malignancies, including breast carcinoma. The objective of this study is to determine the incidence of Akt phosphorylation in breast tumours and its relationship with expression of ER-α, ER-β, HER2, Ki-67 and phosphorylated Bcl-2 associated death domain (p-BAD). Immunohistochemical staining was performed to detect these molecules on 43 paraffin-embedded breast tumour tissues with commercially available antibodies. Eighteen (41.9%), 3 (7.0%), 23 (53.5%), 35 (81.4%), 21 (48.8%), 29 (67.4%), and 34 (81.0%) of breast tumours were positive for nuclear ER-α, nuclear ER-β, membranous HER2, cytonuclear p-Akt (Thr308), p-Akt (Ser473), p-BAD and Ki-67, respectively. ER-α expression was inversely correlated with HER2 and Ki-67 (P=0.041 and P=0.040, respectively). The p-Akt (Ser473) was correlated with increased level of p-BAD (Ser136) (P=0.012). No relationship of Akt phosphorylation with HER2, ER-α or ER-β was found. The p-Akt (Ser473) immunoreactivity was significantly higher in stage IV than in stage I or II (P=0.036 or P=0.009). The higher Ki-67 and lower ER-α expression showed an association with patient age of <50 years (P=0.004) and with positive nodal status(P=0.033), respectively. Our data suggest that the Aktphosphorylation and inactivation of its downstream target, BAD may play a role in survival of breast cancer cell. This study does not support the simple model of linear HER2/ PI3K/Akt pathway in breast cancer. |
| first_indexed | 2025-11-15T08:01:25Z |
| format | Article |
| id | upm-15099 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:01:25Z |
| publishDate | 2010 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-150992016-04-14T05:29:59Z http://psasir.upm.edu.my/id/eprint/15099/ Immunohistochemical detection of phospho-Akt, phospho-BAD, HER2 and oestrogen receptors α and β in Malaysian breast cancer patients Seow, Heng Fong Yip, Wai Kien Loh, Hui Woon Ithnin, Hairuszah Por, Patricia Muhammad, Rohaizak Activation of Akt signaling pathway has been documented in various human malignancies, including breast carcinoma. The objective of this study is to determine the incidence of Akt phosphorylation in breast tumours and its relationship with expression of ER-α, ER-β, HER2, Ki-67 and phosphorylated Bcl-2 associated death domain (p-BAD). Immunohistochemical staining was performed to detect these molecules on 43 paraffin-embedded breast tumour tissues with commercially available antibodies. Eighteen (41.9%), 3 (7.0%), 23 (53.5%), 35 (81.4%), 21 (48.8%), 29 (67.4%), and 34 (81.0%) of breast tumours were positive for nuclear ER-α, nuclear ER-β, membranous HER2, cytonuclear p-Akt (Thr308), p-Akt (Ser473), p-BAD and Ki-67, respectively. ER-α expression was inversely correlated with HER2 and Ki-67 (P=0.041 and P=0.040, respectively). The p-Akt (Ser473) was correlated with increased level of p-BAD (Ser136) (P=0.012). No relationship of Akt phosphorylation with HER2, ER-α or ER-β was found. The p-Akt (Ser473) immunoreactivity was significantly higher in stage IV than in stage I or II (P=0.036 or P=0.009). The higher Ki-67 and lower ER-α expression showed an association with patient age of <50 years (P=0.004) and with positive nodal status(P=0.033), respectively. Our data suggest that the Aktphosphorylation and inactivation of its downstream target, BAD may play a role in survival of breast cancer cell. This study does not support the simple model of linear HER2/ PI3K/Akt pathway in breast cancer. Springer 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15099/1/Immunohistochemical%20detection%20of%20phospho-Akt%2C%20phospho-BAD%2C%20HER2%20and%20oestrogen%20receptors%20%CE%B1%20and%20%CE%B2%20in%20Malaysian%20breast%20cancer%20patients.pdf Seow, Heng Fong and Yip, Wai Kien and Loh, Hui Woon and Ithnin, Hairuszah and Por, Patricia and Muhammad, Rohaizak (2010) Immunohistochemical detection of phospho-Akt, phospho-BAD, HER2 and oestrogen receptors α and β in Malaysian breast cancer patients. Pathology & Oncology Research, 16 (2). pp. 239-248. ISSN 1219-4956; ESSN: 1532-2807 http://link.springer.com/article/10.1007%2Fs12253-009-9216-3 10.1007/s12253-009-9216-3 |
| spellingShingle | Seow, Heng Fong Yip, Wai Kien Loh, Hui Woon Ithnin, Hairuszah Por, Patricia Muhammad, Rohaizak Immunohistochemical detection of phospho-Akt, phospho-BAD, HER2 and oestrogen receptors α and β in Malaysian breast cancer patients |
| title | Immunohistochemical detection of phospho-Akt, phospho-BAD, HER2 and oestrogen receptors α and β in Malaysian breast cancer patients |
| title_full | Immunohistochemical detection of phospho-Akt, phospho-BAD, HER2 and oestrogen receptors α and β in Malaysian breast cancer patients |
| title_fullStr | Immunohistochemical detection of phospho-Akt, phospho-BAD, HER2 and oestrogen receptors α and β in Malaysian breast cancer patients |
| title_full_unstemmed | Immunohistochemical detection of phospho-Akt, phospho-BAD, HER2 and oestrogen receptors α and β in Malaysian breast cancer patients |
| title_short | Immunohistochemical detection of phospho-Akt, phospho-BAD, HER2 and oestrogen receptors α and β in Malaysian breast cancer patients |
| title_sort | immunohistochemical detection of phospho-akt, phospho-bad, her2 and oestrogen receptors α and β in malaysian breast cancer patients |
| url | http://psasir.upm.edu.my/id/eprint/15099/ http://psasir.upm.edu.my/id/eprint/15099/ http://psasir.upm.edu.my/id/eprint/15099/ http://psasir.upm.edu.my/id/eprint/15099/1/Immunohistochemical%20detection%20of%20phospho-Akt%2C%20phospho-BAD%2C%20HER2%20and%20oestrogen%20receptors%20%CE%B1%20and%20%CE%B2%20in%20Malaysian%20breast%20cancer%20patients.pdf |