Immunogenic properties of recombinant ectodomain of Newcastle disease virus hemagglutinin-neuraminidase protein expressed in Escherichia coli.
Hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) plays a vital role in the viral infectivity, host immunity, and disease diagnosis. A portion of the HN gene encoding the ectodomain (nt 142-1739) was cloned and expressed in Escherichia coli yielding an insoluble HN protein an...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Academic Electronic Press Ltd.
2009
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/15097/ http://psasir.upm.edu.my/id/eprint/15097/1/Immunogenic%20properties%20of%20recombinant%20ectodomain%20of%20Newcastle%20disease%20virus%20hemagglutinin.pdf |
| _version_ | 1848842581553709056 |
|---|---|
| author | Wong, Sing King Tan, Wen Siang Omar, Abdul Rahman Tan, Chon Seng Yusoff, Khatijah |
| author_facet | Wong, Sing King Tan, Wen Siang Omar, Abdul Rahman Tan, Chon Seng Yusoff, Khatijah |
| author_sort | Wong, Sing King |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) plays a vital role in the viral infectivity, host immunity, and disease diagnosis. A portion of the HN gene encoding the ectodomain (nt 142-1739) was cloned and expressed in Escherichia coli yielding an insoluble HN protein and a soluble NusA-HN protein containing N-utilization substance A (NusA) fusion component. Both recombinant proteins were purified and used for immunization of chickens. The recombinant HN protein induced higher antibody titers as compared to the recombinant NusA-HN protein. These antibodies were able to react in immunoblot analysis with the corresponding recombinant proteins as well as with the HN protein of NDV. |
| first_indexed | 2025-11-15T08:01:25Z |
| format | Article |
| id | upm-15097 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:01:25Z |
| publishDate | 2009 |
| publisher | Academic Electronic Press Ltd. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-150972015-10-22T01:00:57Z http://psasir.upm.edu.my/id/eprint/15097/ Immunogenic properties of recombinant ectodomain of Newcastle disease virus hemagglutinin-neuraminidase protein expressed in Escherichia coli. Wong, Sing King Tan, Wen Siang Omar, Abdul Rahman Tan, Chon Seng Yusoff, Khatijah Hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) plays a vital role in the viral infectivity, host immunity, and disease diagnosis. A portion of the HN gene encoding the ectodomain (nt 142-1739) was cloned and expressed in Escherichia coli yielding an insoluble HN protein and a soluble NusA-HN protein containing N-utilization substance A (NusA) fusion component. Both recombinant proteins were purified and used for immunization of chickens. The recombinant HN protein induced higher antibody titers as compared to the recombinant NusA-HN protein. These antibodies were able to react in immunoblot analysis with the corresponding recombinant proteins as well as with the HN protein of NDV. Academic Electronic Press Ltd. 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15097/1/Immunogenic%20properties%20of%20recombinant%20ectodomain%20of%20Newcastle%20disease%20virus%20hemagglutinin.pdf Wong, Sing King and Tan, Wen Siang and Omar, Abdul Rahman and Tan, Chon Seng and Yusoff, Khatijah (2009) Immunogenic properties of recombinant ectodomain of Newcastle disease virus hemagglutinin-neuraminidase protein expressed in Escherichia coli. Acta Virologica, 53 (1). pp. 35-41. ISSN 0001-723X 10:4149/av-2009-01-35 English |
| spellingShingle | Wong, Sing King Tan, Wen Siang Omar, Abdul Rahman Tan, Chon Seng Yusoff, Khatijah Immunogenic properties of recombinant ectodomain of Newcastle disease virus hemagglutinin-neuraminidase protein expressed in Escherichia coli. |
| title | Immunogenic properties of recombinant ectodomain of Newcastle disease virus hemagglutinin-neuraminidase protein expressed in Escherichia coli. |
| title_full | Immunogenic properties of recombinant ectodomain of Newcastle disease virus hemagglutinin-neuraminidase protein expressed in Escherichia coli. |
| title_fullStr | Immunogenic properties of recombinant ectodomain of Newcastle disease virus hemagglutinin-neuraminidase protein expressed in Escherichia coli. |
| title_full_unstemmed | Immunogenic properties of recombinant ectodomain of Newcastle disease virus hemagglutinin-neuraminidase protein expressed in Escherichia coli. |
| title_short | Immunogenic properties of recombinant ectodomain of Newcastle disease virus hemagglutinin-neuraminidase protein expressed in Escherichia coli. |
| title_sort | immunogenic properties of recombinant ectodomain of newcastle disease virus hemagglutinin-neuraminidase protein expressed in escherichia coli. |
| url | http://psasir.upm.edu.my/id/eprint/15097/ http://psasir.upm.edu.my/id/eprint/15097/ http://psasir.upm.edu.my/id/eprint/15097/1/Immunogenic%20properties%20of%20recombinant%20ectodomain%20of%20Newcastle%20disease%20virus%20hemagglutinin.pdf |