Immunogenic properties of recombinant ectodomain of Newcastle disease virus hemagglutinin-neuraminidase protein expressed in Escherichia coli.
Hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) plays a vital role in the viral infectivity, host immunity, and disease diagnosis. A portion of the HN gene encoding the ectodomain (nt 142-1739) was cloned and expressed in Escherichia coli yielding an insoluble HN protein an...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Academic Electronic Press Ltd.
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15097/ http://psasir.upm.edu.my/id/eprint/15097/1/Immunogenic%20properties%20of%20recombinant%20ectodomain%20of%20Newcastle%20disease%20virus%20hemagglutinin.pdf |
| Summary: | Hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) plays a vital role in the viral infectivity, host immunity, and disease diagnosis. A portion of the HN gene encoding the ectodomain (nt 142-1739) was cloned and expressed in Escherichia coli yielding an insoluble HN protein and a soluble NusA-HN protein containing N-utilization substance A (NusA) fusion component. Both recombinant proteins were purified and used for immunization of chickens. The recombinant HN protein induced higher antibody titers as compared to the recombinant NusA-HN protein. These antibodies were able to react in immunoblot analysis with the corresponding recombinant proteins as well as with the HN protein of NDV. |
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