Identification of Vibrio parahaemolyticus isolates by PCR targeted to the toxR gene and detection of virulence genes
Vibrio parahaemolyticus is a gram negative bacterium and causes gastrointestinal illness in humans. In this study, twenty five out of fifty cockle samples from Padang, Indonesia produced purple colonies when they were grown on selective medium, CHROMagarTM Vibrio. Specific–PCR for toxR gene detectio...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15084/ http://psasir.upm.edu.my/id/eprint/15084/1/15084.pdf |
| _version_ | 1848842577830215680 |
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| author | Zulkifli, Y. Mohammed Alitheen, Noorjahan Banu Radu, Son Yeap, Swee Keong Bilung, Lesley Maurice Abdul Rahim, Raha |
| author_facet | Zulkifli, Y. Mohammed Alitheen, Noorjahan Banu Radu, Son Yeap, Swee Keong Bilung, Lesley Maurice Abdul Rahim, Raha |
| author_sort | Zulkifli, Y. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Vibrio parahaemolyticus is a gram negative bacterium and causes gastrointestinal illness in humans. In this study, twenty five out of fifty cockle samples from Padang, Indonesia produced purple colonies when they were grown on selective medium, CHROMagarTM Vibrio. Specific–PCR for toxR gene detection gave positive results in which a band with 368 base pairs size appeared on the gel for all the isolates that confirmed the presence of V. parahaemolyticus.
In the virulence properties test, all the isolates showed negative results for tdh and trh genes detection. The results indicate that the isolates under this study do not contain virulence properties that correlate to the ability of infection and diseases, which means that they are nonpathogenic. |
| first_indexed | 2025-11-15T08:01:21Z |
| format | Article |
| id | upm-15084 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:01:21Z |
| publishDate | 2009 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-150842016-04-22T03:52:19Z http://psasir.upm.edu.my/id/eprint/15084/ Identification of Vibrio parahaemolyticus isolates by PCR targeted to the toxR gene and detection of virulence genes Zulkifli, Y. Mohammed Alitheen, Noorjahan Banu Radu, Son Yeap, Swee Keong Bilung, Lesley Maurice Abdul Rahim, Raha Vibrio parahaemolyticus is a gram negative bacterium and causes gastrointestinal illness in humans. In this study, twenty five out of fifty cockle samples from Padang, Indonesia produced purple colonies when they were grown on selective medium, CHROMagarTM Vibrio. Specific–PCR for toxR gene detection gave positive results in which a band with 368 base pairs size appeared on the gel for all the isolates that confirmed the presence of V. parahaemolyticus. In the virulence properties test, all the isolates showed negative results for tdh and trh genes detection. The results indicate that the isolates under this study do not contain virulence properties that correlate to the ability of infection and diseases, which means that they are nonpathogenic. Faculty of Food Science and Technology, Universiti Putra Malaysia 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15084/1/15084.pdf Zulkifli, Y. and Mohammed Alitheen, Noorjahan Banu and Radu, Son and Yeap, Swee Keong and Bilung, Lesley Maurice and Abdul Rahim, Raha (2009) Identification of Vibrio parahaemolyticus isolates by PCR targeted to the toxR gene and detection of virulence genes. International Food Research Journal, 16 (3). pp. 289-296. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/16%20%283%29%202009/3%5B1%5D%20Noorjahan.pdf |
| spellingShingle | Zulkifli, Y. Mohammed Alitheen, Noorjahan Banu Radu, Son Yeap, Swee Keong Bilung, Lesley Maurice Abdul Rahim, Raha Identification of Vibrio parahaemolyticus isolates by PCR targeted to the toxR gene and detection of virulence genes |
| title | Identification of Vibrio parahaemolyticus isolates by PCR targeted to the toxR gene and detection of virulence genes |
| title_full | Identification of Vibrio parahaemolyticus isolates by PCR targeted to the toxR gene and detection of virulence genes |
| title_fullStr | Identification of Vibrio parahaemolyticus isolates by PCR targeted to the toxR gene and detection of virulence genes |
| title_full_unstemmed | Identification of Vibrio parahaemolyticus isolates by PCR targeted to the toxR gene and detection of virulence genes |
| title_short | Identification of Vibrio parahaemolyticus isolates by PCR targeted to the toxR gene and detection of virulence genes |
| title_sort | identification of vibrio parahaemolyticus isolates by pcr targeted to the toxr gene and detection of virulence genes |
| url | http://psasir.upm.edu.my/id/eprint/15084/ http://psasir.upm.edu.my/id/eprint/15084/ http://psasir.upm.edu.my/id/eprint/15084/1/15084.pdf |