Identification of cDNA-RFLP markers and their use for molecular mapping in oil palm (Elaeis guineensis)
Restriction fragment length polymorphic (RFLP) probes derived from complementary DNA (cDNA) clones were developed for oil palm genome analysis. A total of 321 cDNA-RFLP probes were evaluated for their ability to detect polymorphism in a mapping family derived from the selfing of a Tenera guineensis...
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| Format: | Article |
| Language: | English |
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Malaysian Society for Molecular Biology and Biotechnology
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15075/ http://psasir.upm.edu.my/id/eprint/15075/1/Identification%20of%20cDNA-RFLP%20markers%20and%20their%20use%20for%20molecular%20mapping%20in%20oil%20palm%20%28Elaeis%20guineensis%29.pdf |
| _version_ | 1848842575323070464 |
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| author | Singh, Rajinder Tan, Soon Guan Panandam, Jothi Malar Abdul Rahman, Rahimah Cheah, Suan Choo |
| author_facet | Singh, Rajinder Tan, Soon Guan Panandam, Jothi Malar Abdul Rahman, Rahimah Cheah, Suan Choo |
| author_sort | Singh, Rajinder |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Restriction fragment length polymorphic (RFLP) probes derived from complementary DNA (cDNA) clones were developed for oil palm genome analysis. A total of 321 cDNA-RFLP probes were evaluated for their ability to detect polymorphism in a mapping family derived from the selfing of a Tenera guineensis palm (palm T128). Approximately 38% (123 probes) revealed polymorphism with at least one restriction enzyme. All the 123 informative probes were used to genotype the mapping family. Majority of the markers (80%) showed expected segregation ratios, indicating that most of the RFLP markers were inherited in a Mendelian manner. A total of 116 segregating markers were assigned to 20 linkage groups spanning 693cM. The RFLP markers were found to be largely well distributed and did not show excessive clustering in any particular region. This is the first published map for oil palm containing gene specific markers. The cDNA-RFLP probes mapped will not be merely anonymous markers with symbols, but point to the actual location of specific genes. The map also proved useful in revealing QTL associated with oil to wet mesocarp (O/WM) content. |
| first_indexed | 2025-11-15T08:01:19Z |
| format | Article |
| id | upm-15075 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:01:19Z |
| publishDate | 2008 |
| publisher | Malaysian Society for Molecular Biology and Biotechnology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-150752018-01-19T08:26:45Z http://psasir.upm.edu.my/id/eprint/15075/ Identification of cDNA-RFLP markers and their use for molecular mapping in oil palm (Elaeis guineensis) Singh, Rajinder Tan, Soon Guan Panandam, Jothi Malar Abdul Rahman, Rahimah Cheah, Suan Choo Restriction fragment length polymorphic (RFLP) probes derived from complementary DNA (cDNA) clones were developed for oil palm genome analysis. A total of 321 cDNA-RFLP probes were evaluated for their ability to detect polymorphism in a mapping family derived from the selfing of a Tenera guineensis palm (palm T128). Approximately 38% (123 probes) revealed polymorphism with at least one restriction enzyme. All the 123 informative probes were used to genotype the mapping family. Majority of the markers (80%) showed expected segregation ratios, indicating that most of the RFLP markers were inherited in a Mendelian manner. A total of 116 segregating markers were assigned to 20 linkage groups spanning 693cM. The RFLP markers were found to be largely well distributed and did not show excessive clustering in any particular region. This is the first published map for oil palm containing gene specific markers. The cDNA-RFLP probes mapped will not be merely anonymous markers with symbols, but point to the actual location of specific genes. The map also proved useful in revealing QTL associated with oil to wet mesocarp (O/WM) content. Malaysian Society for Molecular Biology and Biotechnology 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15075/1/Identification%20of%20cDNA-RFLP%20markers%20and%20their%20use%20for%20molecular%20mapping%20in%20oil%20palm%20%28Elaeis%20guineensis%29.pdf Singh, Rajinder and Tan, Soon Guan and Panandam, Jothi Malar and Abdul Rahman, Rahimah and Cheah, Suan Choo (2008) Identification of cDNA-RFLP markers and their use for molecular mapping in oil palm (Elaeis guineensis). Asia Pacific Journal of Molecular Biology and Biotechnology, 16 (3). pp. 53-63. ISSN 0128-7451 http://www.msmbb.org.my/apjmbb/html163/163a.htm |
| spellingShingle | Singh, Rajinder Tan, Soon Guan Panandam, Jothi Malar Abdul Rahman, Rahimah Cheah, Suan Choo Identification of cDNA-RFLP markers and their use for molecular mapping in oil palm (Elaeis guineensis) |
| title | Identification of cDNA-RFLP markers and their use for molecular mapping in oil palm (Elaeis guineensis) |
| title_full | Identification of cDNA-RFLP markers and their use for molecular mapping in oil palm (Elaeis guineensis) |
| title_fullStr | Identification of cDNA-RFLP markers and their use for molecular mapping in oil palm (Elaeis guineensis) |
| title_full_unstemmed | Identification of cDNA-RFLP markers and their use for molecular mapping in oil palm (Elaeis guineensis) |
| title_short | Identification of cDNA-RFLP markers and their use for molecular mapping in oil palm (Elaeis guineensis) |
| title_sort | identification of cdna-rflp markers and their use for molecular mapping in oil palm (elaeis guineensis) |
| url | http://psasir.upm.edu.my/id/eprint/15075/ http://psasir.upm.edu.my/id/eprint/15075/ http://psasir.upm.edu.my/id/eprint/15075/1/Identification%20of%20cDNA-RFLP%20markers%20and%20their%20use%20for%20molecular%20mapping%20in%20oil%20palm%20%28Elaeis%20guineensis%29.pdf |