Identification and quantitative determination of glucosinolates in Brassica napus cv. Hanakkori.
The objective of this study was to identify and quantify glucosinolates (GSLs) in Brassica napus cv. Hanakkori and its parents and to evaluate its potential bitter taste. ‘Hanakkori’ materials were cultivated with commercial chemical nutrients (20 kg/ha, N-P-K: 16-10-10) at the field. GSLs were isol...
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| Format: | Article |
| Language: | English English |
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Korean Society of Food Science and Technology
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15074/ http://psasir.upm.edu.my/id/eprint/15074/1/Identification%20and%20quantitative%20determination%20of%20glucosinolates%20in%20Brassica%20napus%20cv.pdf |
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| author | Kim, Sun Ju Fujii, Kouei Sarker, Zaidul Islam Kim, Hyun Wong Yamauchi, Hiroaki Ishii, Gensho |
| author_facet | Kim, Sun Ju Fujii, Kouei Sarker, Zaidul Islam Kim, Hyun Wong Yamauchi, Hiroaki Ishii, Gensho |
| author_sort | Kim, Sun Ju |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The objective of this study was to identify and quantify glucosinolates (GSLs) in Brassica napus cv. Hanakkori and its parents and to evaluate its potential bitter taste. ‘Hanakkori’ materials were cultivated with commercial chemical nutrients (20 kg/ha, N-P-K: 16-10-10) at the field. GSLs were isolated by means of extraction with 70%(v/v) boiling methanol (MeOH) followed by desulfation from those plants by reversed-phase high performance liquid chromatography (HPLC) and identified by electronic spray ionization-mass spectrometry (ESI-MS) analysis. In ‘Hanakkori’, 11 GSLs were identified as progoitrin, glucoraphanin, glucoalyssin, gluconapoleiferin, gluconapin, 1-methylpropyl, glucobrassicanapin, glucobrassicin, 4- methoxyglucobrassicin, gluconasturtiin, and neoglucobrassicin. The total GSL contents were 109 and 36.1 mmol/kg dry weights (d.w.) for the seeds and edible parts, respectively. The major GSLs (>5 mmol/kg d.w.) in the seeds were progoitrin (78.8), gluconapin (10.7), and glucobrassicanapin (7.81), whereas they in the edible parts were progoitrin (16.1) and glucobrassicanapin (8.58). In addition, the bitter taste in the edible parts was presumably related with the presence of progoitrin (>45% to the total GSL). |
| first_indexed | 2025-11-15T08:01:18Z |
| format | Article |
| id | upm-15074 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:01:18Z |
| publishDate | 2008 |
| publisher | Korean Society of Food Science and Technology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-150742015-09-28T07:57:41Z http://psasir.upm.edu.my/id/eprint/15074/ Identification and quantitative determination of glucosinolates in Brassica napus cv. Hanakkori. Kim, Sun Ju Fujii, Kouei Sarker, Zaidul Islam Kim, Hyun Wong Yamauchi, Hiroaki Ishii, Gensho The objective of this study was to identify and quantify glucosinolates (GSLs) in Brassica napus cv. Hanakkori and its parents and to evaluate its potential bitter taste. ‘Hanakkori’ materials were cultivated with commercial chemical nutrients (20 kg/ha, N-P-K: 16-10-10) at the field. GSLs were isolated by means of extraction with 70%(v/v) boiling methanol (MeOH) followed by desulfation from those plants by reversed-phase high performance liquid chromatography (HPLC) and identified by electronic spray ionization-mass spectrometry (ESI-MS) analysis. In ‘Hanakkori’, 11 GSLs were identified as progoitrin, glucoraphanin, glucoalyssin, gluconapoleiferin, gluconapin, 1-methylpropyl, glucobrassicanapin, glucobrassicin, 4- methoxyglucobrassicin, gluconasturtiin, and neoglucobrassicin. The total GSL contents were 109 and 36.1 mmol/kg dry weights (d.w.) for the seeds and edible parts, respectively. The major GSLs (>5 mmol/kg d.w.) in the seeds were progoitrin (78.8), gluconapin (10.7), and glucobrassicanapin (7.81), whereas they in the edible parts were progoitrin (16.1) and glucobrassicanapin (8.58). In addition, the bitter taste in the edible parts was presumably related with the presence of progoitrin (>45% to the total GSL). Korean Society of Food Science and Technology 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15074/1/Identification%20and%20quantitative%20determination%20of%20glucosinolates%20in%20Brassica%20napus%20cv.pdf Kim, Sun Ju and Fujii, Kouei and Sarker, Zaidul Islam and Kim, Hyun Wong and Yamauchi, Hiroaki and Ishii, Gensho (2008) Identification and quantitative determination of glucosinolates in Brassica napus cv. Hanakkori. Food Science and Biotechnology, 17 (5). pp. 1097-1101. ISSN 1226-7708; ESSN: 2092-6456 English |
| spellingShingle | Kim, Sun Ju Fujii, Kouei Sarker, Zaidul Islam Kim, Hyun Wong Yamauchi, Hiroaki Ishii, Gensho Identification and quantitative determination of glucosinolates in Brassica napus cv. Hanakkori. |
| title | Identification and quantitative determination of glucosinolates in Brassica napus cv. Hanakkori. |
| title_full | Identification and quantitative determination of glucosinolates in Brassica napus cv. Hanakkori. |
| title_fullStr | Identification and quantitative determination of glucosinolates in Brassica napus cv. Hanakkori. |
| title_full_unstemmed | Identification and quantitative determination of glucosinolates in Brassica napus cv. Hanakkori. |
| title_short | Identification and quantitative determination of glucosinolates in Brassica napus cv. Hanakkori. |
| title_sort | identification and quantitative determination of glucosinolates in brassica napus cv. hanakkori. |
| url | http://psasir.upm.edu.my/id/eprint/15074/ http://psasir.upm.edu.my/id/eprint/15074/1/Identification%20and%20quantitative%20determination%20of%20glucosinolates%20in%20Brassica%20napus%20cv.pdf |