Identification and characterization of actinomycetes for biological control of bacterial wilt of Ralstonia solanacearum isolated from tomato
Five actinomycetes which showed antimicrobial activity towards Ralstonia solanacearum were identified using specific polymerase chain reaction (PCR) of 16S rDNA gene. Strain C1 and Strain G10 were identified as Streptomyces aureofaciens and S. roseoflavus respectively. All actinomycetes were then ch...
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| Format: | Article |
| Language: | English |
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Malaysian Agricultural Research and Development Institute (MARDI)
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15072/ http://psasir.upm.edu.my/id/eprint/15072/1/Identification%20and%20characterization%20of%20actinomycetes%20for%20biological%20control%20of%20bacterial%20wilt%20of%20ralstonia%20solanacearum%20isolated%20from%20tomato.pdf |
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| author | Abd Mutalib, Sahilah Tang, Sui Yan Mohamed Neji, Zaimawati Hassan, Rosnah Md. Shah, Umi Kalsom Radu, Son |
| author_facet | Abd Mutalib, Sahilah Tang, Sui Yan Mohamed Neji, Zaimawati Hassan, Rosnah Md. Shah, Umi Kalsom Radu, Son |
| author_sort | Abd Mutalib, Sahilah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Five actinomycetes which showed antimicrobial activity towards Ralstonia solanacearum were identified using specific polymerase chain reaction (PCR) of 16S rDNA gene. Strain C1 and Strain G10 were identified as Streptomyces aureofaciens and S. roseoflavus respectively. All actinomycetes were then characterized using antimicrobial and extracellular enzyme activity, metabolic and restriction fragment length polymorphism (RFLP) profiles. Strain A3 showed positive reaction to three bacteria namely Xanthomonas campestris, Staphylococcus aureus and Listeria monocytogenes. Strain C1 and Strain I15 showed positive reaction towards S. aureus and X. campestris respectively. Strains A3, C1 and I15 were able to metabolize xylan and cellulose, while Strain G10 and Strain L8 were able to use all substrates (xylan, mannan and cellulose) as carbon sources. All the Streptomyces strains were positive towards more than 25 carbon sources and can be differentiated into five distinct strains. These results were consistent and confirmed with DNA analysis of RFLP profiles. The specific amplification of 16S rDNA PCR restriction profiles for the strains using three restriction endonucleases, resulted two restriction profiles produced from the digested 16S rDNA product using HaeIII (H1–H2) and HinfI (Hf1–Hf2), while PstI produced three restriction profiles (P1–P3). No profiles were produced from restriction endonuclases of XbaI, SpeI and BamHI. |
| first_indexed | 2025-11-15T08:01:18Z |
| format | Article |
| id | upm-15072 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:01:18Z |
| publishDate | 2010 |
| publisher | Malaysian Agricultural Research and Development Institute (MARDI) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-150722016-10-10T03:13:33Z http://psasir.upm.edu.my/id/eprint/15072/ Identification and characterization of actinomycetes for biological control of bacterial wilt of Ralstonia solanacearum isolated from tomato Abd Mutalib, Sahilah Tang, Sui Yan Mohamed Neji, Zaimawati Hassan, Rosnah Md. Shah, Umi Kalsom Radu, Son Five actinomycetes which showed antimicrobial activity towards Ralstonia solanacearum were identified using specific polymerase chain reaction (PCR) of 16S rDNA gene. Strain C1 and Strain G10 were identified as Streptomyces aureofaciens and S. roseoflavus respectively. All actinomycetes were then characterized using antimicrobial and extracellular enzyme activity, metabolic and restriction fragment length polymorphism (RFLP) profiles. Strain A3 showed positive reaction to three bacteria namely Xanthomonas campestris, Staphylococcus aureus and Listeria monocytogenes. Strain C1 and Strain I15 showed positive reaction towards S. aureus and X. campestris respectively. Strains A3, C1 and I15 were able to metabolize xylan and cellulose, while Strain G10 and Strain L8 were able to use all substrates (xylan, mannan and cellulose) as carbon sources. All the Streptomyces strains were positive towards more than 25 carbon sources and can be differentiated into five distinct strains. These results were consistent and confirmed with DNA analysis of RFLP profiles. The specific amplification of 16S rDNA PCR restriction profiles for the strains using three restriction endonucleases, resulted two restriction profiles produced from the digested 16S rDNA product using HaeIII (H1–H2) and HinfI (Hf1–Hf2), while PstI produced three restriction profiles (P1–P3). No profiles were produced from restriction endonuclases of XbaI, SpeI and BamHI. Malaysian Agricultural Research and Development Institute (MARDI) 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15072/1/Identification%20and%20characterization%20of%20actinomycetes%20for%20biological%20control%20of%20bacterial%20wilt%20of%20ralstonia%20solanacearum%20isolated%20from%20tomato.pdf Abd Mutalib, Sahilah and Tang, Sui Yan and Mohamed Neji, Zaimawati and Hassan, Rosnah and Md. Shah, Umi Kalsom and Radu, Son (2010) Identification and characterization of actinomycetes for biological control of bacterial wilt of Ralstonia solanacearum isolated from tomato. Journal of Tropical Agriculture and Food Science, 38 (1). pp. 103-114. ISSN 1394-9829; ESSN: 2289-9650 http://ejtafs.mardi.gov.my/index.php/publication/issues/archive/27-2010/volume-38-no1/51-380112 |
| spellingShingle | Abd Mutalib, Sahilah Tang, Sui Yan Mohamed Neji, Zaimawati Hassan, Rosnah Md. Shah, Umi Kalsom Radu, Son Identification and characterization of actinomycetes for biological control of bacterial wilt of Ralstonia solanacearum isolated from tomato |
| title | Identification and characterization of actinomycetes for biological control of bacterial wilt of Ralstonia solanacearum isolated from tomato |
| title_full | Identification and characterization of actinomycetes for biological control of bacterial wilt of Ralstonia solanacearum isolated from tomato |
| title_fullStr | Identification and characterization of actinomycetes for biological control of bacterial wilt of Ralstonia solanacearum isolated from tomato |
| title_full_unstemmed | Identification and characterization of actinomycetes for biological control of bacterial wilt of Ralstonia solanacearum isolated from tomato |
| title_short | Identification and characterization of actinomycetes for biological control of bacterial wilt of Ralstonia solanacearum isolated from tomato |
| title_sort | identification and characterization of actinomycetes for biological control of bacterial wilt of ralstonia solanacearum isolated from tomato |
| url | http://psasir.upm.edu.my/id/eprint/15072/ http://psasir.upm.edu.my/id/eprint/15072/ http://psasir.upm.edu.my/id/eprint/15072/1/Identification%20and%20characterization%20of%20actinomycetes%20for%20biological%20control%20of%20bacterial%20wilt%20of%20ralstonia%20solanacearum%20isolated%20from%20tomato.pdf |