High frequency plant regeneration from mature seed of elite, recalcitrant Malaysian indica rice (Oryza sativa L.) CV. MR 219
An efficient in vitro plant regeneration system was established for elite, recalcitrant Malaysian indica rice, Oryza sativa L. CV. MR 219 using mature seeds as explant on Murashige and Skoog and Chu N6 media containing 2,4-dichlorophenoxy acetic acid and kinetin either alone or in different combinat...
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Akademiai Kiado
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15004/ http://psasir.upm.edu.my/id/eprint/15004/1/High%20frequency%20plant%20regeneration%20from%20mature%20seed%20of%20elite%2C%20recalcitrant%20Malaysian%20indica%20rice%20%28Oryza%20sativa%20L.%29%20CV.%20MR%20219.pdf |
| _version_ | 1848842555852062720 |
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| author | Paramasivam, Sivakumar Law, Yee Song Ho, Chai Ling Harikrishna, Jennifer Ann |
| author_facet | Paramasivam, Sivakumar Law, Yee Song Ho, Chai Ling Harikrishna, Jennifer Ann |
| author_sort | Paramasivam, Sivakumar |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | An efficient in vitro plant regeneration system was established for elite, recalcitrant Malaysian indica rice, Oryza sativa L. CV. MR 219 using mature seeds as explant on Murashige and Skoog and Chu N6 media containing 2,4-dichlorophenoxy acetic acid and kinetin either alone or in different combinations. L-proline, casein hydrolysate and L-glutamine were added to callus induction media for enhancement of embryogenic callus induction. The highest frequency of friable callus induction (84%) was observed in N6 medium containing 2.5 mg l -1 2,4-dichlorophenoxy acetic acid, 0.2 mg l -1 kinetin, 2.5 mg l -1 L-proline, 300 mg l -1 casein hydrolysate, 20 mg l -1 L-glutamine and 30 g l -1 sucrose under culture in continuous lighting conditions. The maximum regeneration frequency (71%) was observed, when 30-day-old N6 friable calli were cultured on MS medium supplemented with 3 mg l -1 6-benzyl aminopurine, 1 mg l -1 naphthalene acetic acid, 2.5 mg l -1 L-proline, 300 mg l -1 casein hydrolysate and 3% maltose. Developed shoots were rooted in half strength MS medium supplemented with 2% sucrose and were successfully transplanted to soil with 95% survival. This protocol may be used for other recalcitrant indica rice genotypes and to transfer desirable genes in to Malaysian indica rice cultivar MR219 for crop improvement. |
| first_indexed | 2025-11-15T08:01:00Z |
| format | Article |
| id | upm-15004 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:01:00Z |
| publishDate | 2010 |
| publisher | Akademiai Kiado |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-150042019-10-22T03:25:03Z http://psasir.upm.edu.my/id/eprint/15004/ High frequency plant regeneration from mature seed of elite, recalcitrant Malaysian indica rice (Oryza sativa L.) CV. MR 219 Paramasivam, Sivakumar Law, Yee Song Ho, Chai Ling Harikrishna, Jennifer Ann An efficient in vitro plant regeneration system was established for elite, recalcitrant Malaysian indica rice, Oryza sativa L. CV. MR 219 using mature seeds as explant on Murashige and Skoog and Chu N6 media containing 2,4-dichlorophenoxy acetic acid and kinetin either alone or in different combinations. L-proline, casein hydrolysate and L-glutamine were added to callus induction media for enhancement of embryogenic callus induction. The highest frequency of friable callus induction (84%) was observed in N6 medium containing 2.5 mg l -1 2,4-dichlorophenoxy acetic acid, 0.2 mg l -1 kinetin, 2.5 mg l -1 L-proline, 300 mg l -1 casein hydrolysate, 20 mg l -1 L-glutamine and 30 g l -1 sucrose under culture in continuous lighting conditions. The maximum regeneration frequency (71%) was observed, when 30-day-old N6 friable calli were cultured on MS medium supplemented with 3 mg l -1 6-benzyl aminopurine, 1 mg l -1 naphthalene acetic acid, 2.5 mg l -1 L-proline, 300 mg l -1 casein hydrolysate and 3% maltose. Developed shoots were rooted in half strength MS medium supplemented with 2% sucrose and were successfully transplanted to soil with 95% survival. This protocol may be used for other recalcitrant indica rice genotypes and to transfer desirable genes in to Malaysian indica rice cultivar MR219 for crop improvement. Akademiai Kiado 2010 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/15004/1/High%20frequency%20plant%20regeneration%20from%20mature%20seed%20of%20elite%2C%20recalcitrant%20Malaysian%20indica%20rice%20%28Oryza%20sativa%20L.%29%20CV.%20MR%20219.pdf Paramasivam, Sivakumar and Law, Yee Song and Ho, Chai Ling and Harikrishna, Jennifer Ann (2010) High frequency plant regeneration from mature seed of elite, recalcitrant Malaysian indica rice (Oryza sativa L.) CV. MR 219. Acta Biologica Hungarica, 61 (3). pp. 313-321. ISSN 0236-5383; ESSN: 1588-256X https://akademiai.com/doi/abs/10.1556/ABiol.61.2010.3.7 10.1556/ABiol.61.2010.3.7 |
| spellingShingle | Paramasivam, Sivakumar Law, Yee Song Ho, Chai Ling Harikrishna, Jennifer Ann High frequency plant regeneration from mature seed of elite, recalcitrant Malaysian indica rice (Oryza sativa L.) CV. MR 219 |
| title | High frequency plant regeneration from mature seed of elite, recalcitrant Malaysian indica rice (Oryza sativa L.) CV. MR 219 |
| title_full | High frequency plant regeneration from mature seed of elite, recalcitrant Malaysian indica rice (Oryza sativa L.) CV. MR 219 |
| title_fullStr | High frequency plant regeneration from mature seed of elite, recalcitrant Malaysian indica rice (Oryza sativa L.) CV. MR 219 |
| title_full_unstemmed | High frequency plant regeneration from mature seed of elite, recalcitrant Malaysian indica rice (Oryza sativa L.) CV. MR 219 |
| title_short | High frequency plant regeneration from mature seed of elite, recalcitrant Malaysian indica rice (Oryza sativa L.) CV. MR 219 |
| title_sort | high frequency plant regeneration from mature seed of elite, recalcitrant malaysian indica rice (oryza sativa l.) cv. mr 219 |
| url | http://psasir.upm.edu.my/id/eprint/15004/ http://psasir.upm.edu.my/id/eprint/15004/ http://psasir.upm.edu.my/id/eprint/15004/ http://psasir.upm.edu.my/id/eprint/15004/1/High%20frequency%20plant%20regeneration%20from%20mature%20seed%20of%20elite%2C%20recalcitrant%20Malaysian%20indica%20rice%20%28Oryza%20sativa%20L.%29%20CV.%20MR%20219.pdf |