Growth kinetics of diazotrophic Bacillus sphaericus UPMB10 cultured using different types and concentrations of carbon and nitrogen sources
Growth kinetics of newly isolated diazotrophic Bacillus sphaericus UPMB10 grown in various carbon (lactate, acetate, glycerol, malate, fructose, xylose and sucrose) and nitrogen (glutamate, yeast extract, arginine, hystadine, glycine, polypeptone, tryptophan, lysine, NH4Cl and urea) sources was inv...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Malaysian Society for Microbiology
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14931/ |
| _version_ | 1848842536266760192 |
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| author | Ooi, T. C. Ariff, Arbakariya Mohd Saud, Halimi Shamsuddin, Zulkifli |
| author_facet | Ooi, T. C. Ariff, Arbakariya Mohd Saud, Halimi Shamsuddin, Zulkifli |
| author_sort | Ooi, T. C. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Growth kinetics of newly isolated diazotrophic Bacillus sphaericus UPMB10 grown in various carbon (lactate, acetate,
glycerol, malate, fructose, xylose and sucrose) and nitrogen (glutamate, yeast extract, arginine, hystadine, glycine, polypeptone, tryptophan, lysine, NH4Cl and urea) sources was investigated using 2 L stirred tank fermenter. The highest growth was obtained in a medium containing lactate as a carbon source, which gave the highest maximum cell concentration of 2.30 g/L, which is corresponding to maximum viable cell count of 4.60 x 109 cfu/mL. However, the
highest cell yield (1.06 g cell/g carbon consumed) was obtained in cultivation using glycerol though slightly lower
maximum viable cell count was obtained (3.22 x 109 cfu/mL). In addition, cost for the production of live cell using glycerol was about 15 times lower than the cost using lactate. Growth performance of this bacterium when yeast extract was used as a nitrogen source was comparable to the use of pure amino acid. The medium containing 1.8 g/L glycerol and 2 g/L yeast extract was suggested as optimal for growth of this bacterium, which gave carbon to nitrogen ratio (C/N) of 10:1. The maximum viable cell count obtained in cultivation using optimised medium in 2 L stirred tank fermenter was 3.34 x 109 cfu/mL and the cells maintained its capacity for N2 fixation at 18 nmol C2H2/h.mL. |
| first_indexed | 2025-11-15T08:00:41Z |
| format | Article |
| id | upm-14931 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:00:41Z |
| publishDate | 2008 |
| publisher | Malaysian Society for Microbiology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-149312015-05-22T03:22:11Z http://psasir.upm.edu.my/id/eprint/14931/ Growth kinetics of diazotrophic Bacillus sphaericus UPMB10 cultured using different types and concentrations of carbon and nitrogen sources Ooi, T. C. Ariff, Arbakariya Mohd Saud, Halimi Shamsuddin, Zulkifli Growth kinetics of newly isolated diazotrophic Bacillus sphaericus UPMB10 grown in various carbon (lactate, acetate, glycerol, malate, fructose, xylose and sucrose) and nitrogen (glutamate, yeast extract, arginine, hystadine, glycine, polypeptone, tryptophan, lysine, NH4Cl and urea) sources was investigated using 2 L stirred tank fermenter. The highest growth was obtained in a medium containing lactate as a carbon source, which gave the highest maximum cell concentration of 2.30 g/L, which is corresponding to maximum viable cell count of 4.60 x 109 cfu/mL. However, the highest cell yield (1.06 g cell/g carbon consumed) was obtained in cultivation using glycerol though slightly lower maximum viable cell count was obtained (3.22 x 109 cfu/mL). In addition, cost for the production of live cell using glycerol was about 15 times lower than the cost using lactate. Growth performance of this bacterium when yeast extract was used as a nitrogen source was comparable to the use of pure amino acid. The medium containing 1.8 g/L glycerol and 2 g/L yeast extract was suggested as optimal for growth of this bacterium, which gave carbon to nitrogen ratio (C/N) of 10:1. The maximum viable cell count obtained in cultivation using optimised medium in 2 L stirred tank fermenter was 3.34 x 109 cfu/mL and the cells maintained its capacity for N2 fixation at 18 nmol C2H2/h.mL. Malaysian Society for Microbiology 2008 Article PeerReviewed Ooi, T. C. and Ariff, Arbakariya and Mohd Saud, Halimi and Shamsuddin, Zulkifli (2008) Growth kinetics of diazotrophic Bacillus sphaericus UPMB10 cultured using different types and concentrations of carbon and nitrogen sources. Malaysian Journal of Microbiology, 4 (2). pp. 15-25. ISSN 1823-8262 Bacillus sphaericus Bacillus (Bacteria) Growth kinetics English |
| spellingShingle | Bacillus sphaericus Bacillus (Bacteria) Growth kinetics Ooi, T. C. Ariff, Arbakariya Mohd Saud, Halimi Shamsuddin, Zulkifli Growth kinetics of diazotrophic Bacillus sphaericus UPMB10 cultured using different types and concentrations of carbon and nitrogen sources |
| title | Growth kinetics of diazotrophic Bacillus sphaericus UPMB10 cultured using different types and concentrations of carbon and nitrogen sources |
| title_full | Growth kinetics of diazotrophic Bacillus sphaericus UPMB10 cultured using different types and concentrations of carbon and nitrogen sources |
| title_fullStr | Growth kinetics of diazotrophic Bacillus sphaericus UPMB10 cultured using different types and concentrations of carbon and nitrogen sources |
| title_full_unstemmed | Growth kinetics of diazotrophic Bacillus sphaericus UPMB10 cultured using different types and concentrations of carbon and nitrogen sources |
| title_short | Growth kinetics of diazotrophic Bacillus sphaericus UPMB10 cultured using different types and concentrations of carbon and nitrogen sources |
| title_sort | growth kinetics of diazotrophic bacillus sphaericus upmb10 cultured using different types and concentrations of carbon and nitrogen sources |
| topic | Bacillus sphaericus Bacillus (Bacteria) Growth kinetics |
| url | http://psasir.upm.edu.my/id/eprint/14931/ |