Genetic characterization of wild stocks of prawns macrobrachium rosenbergii using random polymorphic DNA markers.

Random amplified polymorphic DNA was used to study the genetic diversity and assay polymorphisms among eleven populations of Macrobrachium rosenbergii in Malaysia. The RAPD procedure generated 205 markers in the 11 populations. and a large level of polymorphisms was observed in all populations. The...

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Main Authors: See, Leng Min, Hassan, Rosli, Tan, S.G., Bhassu, Subha
Format: Article
Language:English
Published: Asian Network for Scientific Information (ANSINET) 2008
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/14876/
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author See, Leng Min
Hassan, Rosli
Tan, S.G.
Bhassu, Subha
author_facet See, Leng Min
Hassan, Rosli
Tan, S.G.
Bhassu, Subha
author_sort See, Leng Min
building UPM Institutional Repository
collection Online Access
description Random amplified polymorphic DNA was used to study the genetic diversity and assay polymorphisms among eleven populations of Macrobrachium rosenbergii in Malaysia. The RAPD procedure generated 205 markers in the 11 populations. and a large level of polymorphisms was observed in all populations. The unweighted pair group method with arithmetic averaging (UPGMA) clustering procedure based on the markers identified three major clusters among all populations. The results of the present study showed that RAPD based fingerprinting were a useful tool to assess the genetic variability of freshwater prawn, M. rosenbergii. Assessing high genetic variability within and among M. rosenbergii is considered important genetic resources as it has direct benefit for conserving wild stocks, greater potential for improvement and serves as invaluable resource for different selection criteria, especially when planning breeding or crossbreeding programs.
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spelling upm-148762012-08-02T04:16:18Z http://psasir.upm.edu.my/id/eprint/14876/ Genetic characterization of wild stocks of prawns macrobrachium rosenbergii using random polymorphic DNA markers. See, Leng Min Hassan, Rosli Tan, S.G. Bhassu, Subha Random amplified polymorphic DNA was used to study the genetic diversity and assay polymorphisms among eleven populations of Macrobrachium rosenbergii in Malaysia. The RAPD procedure generated 205 markers in the 11 populations. and a large level of polymorphisms was observed in all populations. The unweighted pair group method with arithmetic averaging (UPGMA) clustering procedure based on the markers identified three major clusters among all populations. The results of the present study showed that RAPD based fingerprinting were a useful tool to assess the genetic variability of freshwater prawn, M. rosenbergii. Assessing high genetic variability within and among M. rosenbergii is considered important genetic resources as it has direct benefit for conserving wild stocks, greater potential for improvement and serves as invaluable resource for different selection criteria, especially when planning breeding or crossbreeding programs. Asian Network for Scientific Information (ANSINET) 2008 Article PeerReviewed See, Leng Min and Hassan, Rosli and Tan, S.G. and Bhassu, Subha (2008) Genetic characterization of wild stocks of prawns macrobrachium rosenbergii using random polymorphic DNA markers. Biotechnology, 7 (2). pp. 338-342. ISSN 1682-296X http://scialert.net/abstract/?doi=biotech.2008.338.342 Macrobrachium rosenbergii. Macrobrachium rosenbergii - Genetics. Shrimp culture. DOI: 10.3923/biotech.2008.338.342 English
spellingShingle Macrobrachium rosenbergii.
Macrobrachium rosenbergii - Genetics.
Shrimp culture.
See, Leng Min
Hassan, Rosli
Tan, S.G.
Bhassu, Subha
Genetic characterization of wild stocks of prawns macrobrachium rosenbergii using random polymorphic DNA markers.
title Genetic characterization of wild stocks of prawns macrobrachium rosenbergii using random polymorphic DNA markers.
title_full Genetic characterization of wild stocks of prawns macrobrachium rosenbergii using random polymorphic DNA markers.
title_fullStr Genetic characterization of wild stocks of prawns macrobrachium rosenbergii using random polymorphic DNA markers.
title_full_unstemmed Genetic characterization of wild stocks of prawns macrobrachium rosenbergii using random polymorphic DNA markers.
title_short Genetic characterization of wild stocks of prawns macrobrachium rosenbergii using random polymorphic DNA markers.
title_sort genetic characterization of wild stocks of prawns macrobrachium rosenbergii using random polymorphic dna markers.
topic Macrobrachium rosenbergii.
Macrobrachium rosenbergii - Genetics.
Shrimp culture.
url http://psasir.upm.edu.my/id/eprint/14876/
http://psasir.upm.edu.my/id/eprint/14876/
http://psasir.upm.edu.my/id/eprint/14876/