GC/MS determination of bioactive components and antibacterial properties of Goniothalamus umbrosus extracts

In this study, the antibacterial activity and chemical composition of Goniothalamus umbrosus leaves extracts were evaluated. The antibacterial activity was investigated using two gram-positive bacteria, Methicillin resistant Staphylococcus aureus (MRSA) and Bacillus subtilis B29, and two gram-negati...

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Main Authors: Abdelwahab, Siddig Ibrahim, Abdul, Ahmad Bustamam, Elhassan Taha, Manal Mohamed, Mohan, Syam, Ibrahim, Mohamed Yousif, Mariod, Abdalbasit Adam, Abdullah Al-Haj, Nagi Ahmed, Abdullah, Rasedee
Format: Article
Language:English
Published: Academic Journals 2009
Online Access:http://psasir.upm.edu.my/id/eprint/14852/
http://psasir.upm.edu.my/id/eprint/14852/1/14852.pdf
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author Abdelwahab, Siddig Ibrahim
Abdul, Ahmad Bustamam
Elhassan Taha, Manal Mohamed
Mohan, Syam
Ibrahim, Mohamed Yousif
Mariod, Abdalbasit Adam
Abdullah Al-Haj, Nagi Ahmed
Abdullah, Rasedee
author_facet Abdelwahab, Siddig Ibrahim
Abdul, Ahmad Bustamam
Elhassan Taha, Manal Mohamed
Mohan, Syam
Ibrahim, Mohamed Yousif
Mariod, Abdalbasit Adam
Abdullah Al-Haj, Nagi Ahmed
Abdullah, Rasedee
author_sort Abdelwahab, Siddig Ibrahim
building UPM Institutional Repository
collection Online Access
description In this study, the antibacterial activity and chemical composition of Goniothalamus umbrosus leaves extracts were evaluated. The antibacterial activity was investigated using two gram-positive bacteria, Methicillin resistant Staphylococcus aureus (MRSA) and Bacillus subtilis B29, and two gram-negative bacteria,Pseudomonas aeruginosa 60690 and Salmonella choleraesuis. The activity was tested using disc diffusion and minimum inhibitory concentration assays. The chemical compositions of the ethyl acetate extract of G. umbrosus were investigated using Shimadzu gas chromatography–mass spectrometry (GC-17A) while the mass spectra of the compounds found in the extract was matched with the library. The results showed that the extracts demonstrated broad spectrum antibacterial effects against all tested bacteria. GC/MS analysis of ethyl acetate extract of G. umbrosus revealed the existence of 1-butyl-2-cyclohexen-1-ol (46.84%), benzaldehyde (4.42%) and globulol (4.07%). The results of this study offer a platform of using G. umbrosus as herbal alternative for the current synthetic antimicrobial agents.
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publishDate 2009
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spelling upm-148522017-11-09T05:34:03Z http://psasir.upm.edu.my/id/eprint/14852/ GC/MS determination of bioactive components and antibacterial properties of Goniothalamus umbrosus extracts Abdelwahab, Siddig Ibrahim Abdul, Ahmad Bustamam Elhassan Taha, Manal Mohamed Mohan, Syam Ibrahim, Mohamed Yousif Mariod, Abdalbasit Adam Abdullah Al-Haj, Nagi Ahmed Abdullah, Rasedee In this study, the antibacterial activity and chemical composition of Goniothalamus umbrosus leaves extracts were evaluated. The antibacterial activity was investigated using two gram-positive bacteria, Methicillin resistant Staphylococcus aureus (MRSA) and Bacillus subtilis B29, and two gram-negative bacteria,Pseudomonas aeruginosa 60690 and Salmonella choleraesuis. The activity was tested using disc diffusion and minimum inhibitory concentration assays. The chemical compositions of the ethyl acetate extract of G. umbrosus were investigated using Shimadzu gas chromatography–mass spectrometry (GC-17A) while the mass spectra of the compounds found in the extract was matched with the library. The results showed that the extracts demonstrated broad spectrum antibacterial effects against all tested bacteria. GC/MS analysis of ethyl acetate extract of G. umbrosus revealed the existence of 1-butyl-2-cyclohexen-1-ol (46.84%), benzaldehyde (4.42%) and globulol (4.07%). The results of this study offer a platform of using G. umbrosus as herbal alternative for the current synthetic antimicrobial agents. Academic Journals 2009-07-20 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14852/1/14852.pdf Abdelwahab, Siddig Ibrahim and Abdul, Ahmad Bustamam and Elhassan Taha, Manal Mohamed and Mohan, Syam and Ibrahim, Mohamed Yousif and Mariod, Abdalbasit Adam and Abdullah Al-Haj, Nagi Ahmed and Abdullah, Rasedee (2009) GC/MS determination of bioactive components and antibacterial properties of Goniothalamus umbrosus extracts. African Journal of Biotechnology, 8 (14). art. no. 339E80715928. pp. 3336-3340. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/339E80715928
spellingShingle Abdelwahab, Siddig Ibrahim
Abdul, Ahmad Bustamam
Elhassan Taha, Manal Mohamed
Mohan, Syam
Ibrahim, Mohamed Yousif
Mariod, Abdalbasit Adam
Abdullah Al-Haj, Nagi Ahmed
Abdullah, Rasedee
GC/MS determination of bioactive components and antibacterial properties of Goniothalamus umbrosus extracts
title GC/MS determination of bioactive components and antibacterial properties of Goniothalamus umbrosus extracts
title_full GC/MS determination of bioactive components and antibacterial properties of Goniothalamus umbrosus extracts
title_fullStr GC/MS determination of bioactive components and antibacterial properties of Goniothalamus umbrosus extracts
title_full_unstemmed GC/MS determination of bioactive components and antibacterial properties of Goniothalamus umbrosus extracts
title_short GC/MS determination of bioactive components and antibacterial properties of Goniothalamus umbrosus extracts
title_sort gc/ms determination of bioactive components and antibacterial properties of goniothalamus umbrosus extracts
url http://psasir.upm.edu.my/id/eprint/14852/
http://psasir.upm.edu.my/id/eprint/14852/
http://psasir.upm.edu.my/id/eprint/14852/1/14852.pdf