Flavonoid combinations cause synergistic inhibition of proinflammatory mediator secretion from lipopolysaccharide-induced RAW 264.7 cells.
Objectives: We evaluated several flavonoid combinations for synergy in the inhibition of proinflammatory mediator synthesis in the RAW 264.7 cellular model of inflammation. Methods: The inhibitory effect of chrysin, kaempferol, morin, silibinin, quercetin, diosmin and hesperidin upon nitric...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English English |
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Springer Verlag
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14777/ http://psasir.upm.edu.my/id/eprint/14777/1/Flavonoid%20combinations%20cause%20synergistic%20inhibition%20of%20proinflammatory%20mediator%20secretion%20from%20lipopolysaccharide.pdf |
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| author | Harasstani, Omar A. Moin, Saidi Tham, Chau Ling Liew, Choi Yi Ismail, Norazren A. Rajajendram, Revathee Harith, Hanis Hazeera Zakaria, Zainul Amiruddin Tengku Mohamad, Tengku Azam Shah Sulaiman, Mohd Roslan Israf Ali, Daud Ahmad |
| author_facet | Harasstani, Omar A. Moin, Saidi Tham, Chau Ling Liew, Choi Yi Ismail, Norazren A. Rajajendram, Revathee Harith, Hanis Hazeera Zakaria, Zainul Amiruddin Tengku Mohamad, Tengku Azam Shah Sulaiman, Mohd Roslan Israf Ali, Daud Ahmad |
| author_sort | Harasstani, Omar A. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Objectives:
We evaluated several flavonoid combinations for synergy in the inhibition of proinflammatory mediator synthesis in the RAW 264.7 cellular model of inflammation.
Methods:
The inhibitory effect of chrysin, kaempferol, morin, silibinin, quercetin, diosmin and hesperidin upon nitric oxide (NO), prostaglandin E2 (PGE2) and tumour necrosis factor-α (TNF-α) secretion from the LPS-induced RAW 264.7 monocytic macrophage was assessed and IC50 values obtained. Flavonoids that showed reasonable inhibitory effects in at least two out of the three assays were combined in a series of fixed IC50 ratios and reassessed for inhibition of NO, PGE2 and TNF-α. Dose-response curves were generated and interactions were analysed using isobolographic analysis.
Results:
The experiments showed that only chrysin, kaempferol, morin, and silibinin were potent enough to produce dose-response effects upon at least two out of the three mediators assayed. Combinations of these four flavonoids showed that several combinations afforded highly significant synergistic effects. Conclusions: Some flavonoids are synergistic in their anti-inflammatory effects when combined. In particular chrysin and kaempferol significantly synergised in their inhibitory effect upon NO, PGE2 and TNF-α secretion. These findings open further avenues of research into combinatorial therapeutics of inflammatory-related diseases and the pharmacology of flavonoid synergy. |
| first_indexed | 2025-11-15T07:59:56Z |
| format | Article |
| id | upm-14777 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:59:56Z |
| publishDate | 2010 |
| publisher | Springer Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-147772015-09-14T08:07:44Z http://psasir.upm.edu.my/id/eprint/14777/ Flavonoid combinations cause synergistic inhibition of proinflammatory mediator secretion from lipopolysaccharide-induced RAW 264.7 cells. Harasstani, Omar A. Moin, Saidi Tham, Chau Ling Liew, Choi Yi Ismail, Norazren A. Rajajendram, Revathee Harith, Hanis Hazeera Zakaria, Zainul Amiruddin Tengku Mohamad, Tengku Azam Shah Sulaiman, Mohd Roslan Israf Ali, Daud Ahmad Objectives: We evaluated several flavonoid combinations for synergy in the inhibition of proinflammatory mediator synthesis in the RAW 264.7 cellular model of inflammation. Methods: The inhibitory effect of chrysin, kaempferol, morin, silibinin, quercetin, diosmin and hesperidin upon nitric oxide (NO), prostaglandin E2 (PGE2) and tumour necrosis factor-α (TNF-α) secretion from the LPS-induced RAW 264.7 monocytic macrophage was assessed and IC50 values obtained. Flavonoids that showed reasonable inhibitory effects in at least two out of the three assays were combined in a series of fixed IC50 ratios and reassessed for inhibition of NO, PGE2 and TNF-α. Dose-response curves were generated and interactions were analysed using isobolographic analysis. Results: The experiments showed that only chrysin, kaempferol, morin, and silibinin were potent enough to produce dose-response effects upon at least two out of the three mediators assayed. Combinations of these four flavonoids showed that several combinations afforded highly significant synergistic effects. Conclusions: Some flavonoids are synergistic in their anti-inflammatory effects when combined. In particular chrysin and kaempferol significantly synergised in their inhibitory effect upon NO, PGE2 and TNF-α secretion. These findings open further avenues of research into combinatorial therapeutics of inflammatory-related diseases and the pharmacology of flavonoid synergy. Springer Verlag 2010-09 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14777/1/Flavonoid%20combinations%20cause%20synergistic%20inhibition%20of%20proinflammatory%20mediator%20secretion%20from%20lipopolysaccharide.pdf Harasstani, Omar A. and Moin, Saidi and Tham, Chau Ling and Liew, Choi Yi and Ismail, Norazren A. and Rajajendram, Revathee and Harith, Hanis Hazeera and Zakaria, Zainul Amiruddin and Tengku Mohamad, Tengku Azam Shah and Sulaiman, Mohd Roslan and Israf Ali, Daud Ahmad (2010) Flavonoid combinations cause synergistic inhibition of proinflammatory mediator secretion from lipopolysaccharide-induced RAW 264.7 cells. Inflammation Research, 59 (9). pp. 711-721. ISSN 1023-3830, ESSN: 1420-908X http://link.springer.com/ 10.1007/s00011-010-0182-8 English |
| spellingShingle | Harasstani, Omar A. Moin, Saidi Tham, Chau Ling Liew, Choi Yi Ismail, Norazren A. Rajajendram, Revathee Harith, Hanis Hazeera Zakaria, Zainul Amiruddin Tengku Mohamad, Tengku Azam Shah Sulaiman, Mohd Roslan Israf Ali, Daud Ahmad Flavonoid combinations cause synergistic inhibition of proinflammatory mediator secretion from lipopolysaccharide-induced RAW 264.7 cells. |
| title | Flavonoid combinations cause synergistic inhibition of proinflammatory mediator secretion from lipopolysaccharide-induced RAW 264.7 cells. |
| title_full | Flavonoid combinations cause synergistic inhibition of proinflammatory mediator secretion from lipopolysaccharide-induced RAW 264.7 cells. |
| title_fullStr | Flavonoid combinations cause synergistic inhibition of proinflammatory mediator secretion from lipopolysaccharide-induced RAW 264.7 cells. |
| title_full_unstemmed | Flavonoid combinations cause synergistic inhibition of proinflammatory mediator secretion from lipopolysaccharide-induced RAW 264.7 cells. |
| title_short | Flavonoid combinations cause synergistic inhibition of proinflammatory mediator secretion from lipopolysaccharide-induced RAW 264.7 cells. |
| title_sort | flavonoid combinations cause synergistic inhibition of proinflammatory mediator secretion from lipopolysaccharide-induced raw 264.7 cells. |
| url | http://psasir.upm.edu.my/id/eprint/14777/ http://psasir.upm.edu.my/id/eprint/14777/ http://psasir.upm.edu.my/id/eprint/14777/ http://psasir.upm.edu.my/id/eprint/14777/1/Flavonoid%20combinations%20cause%20synergistic%20inhibition%20of%20proinflammatory%20mediator%20secretion%20from%20lipopolysaccharide.pdf |