Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia.
A study was conducted to improve 9-methoxycanthin-6-one productivity (potential anti-tumour compound) from callus cultures of Eurycoma longifolia (Tongkat Ali). Several factors affecting 9-methoxycanthin-6-one production in callus cultures such as different medium compositions and physical factors w...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
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Springer Verlag
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14696/ http://psasir.upm.edu.my/id/eprint/14696/1/Factors%20affecting%20the%20accumulation%20of%209.pdf |
| _version_ | 1848842465881096192 |
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| author | Rosli, N. Mahmood, Maziah Chan, K. L. Sreeramanan, S. |
| author_facet | Rosli, N. Mahmood, Maziah Chan, K. L. Sreeramanan, S. |
| author_sort | Rosli, N. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A study was conducted to improve 9-methoxycanthin-6-one productivity (potential anti-tumour compound) from callus cultures of Eurycoma longifolia (Tongkat Ali). Several factors affecting 9-methoxycanthin-6-one production in callus cultures such as different medium compositions and physical factors were investigated and analyzed. Results show that a higher production of 9-methoxycanthin-6-one (3.84 mg'g-1 DW (Dry Weight)) is obtained from callus cultured in ¼ MS basal media. At fructose of 2% (w/v), the production of 9-methoxycanthin-6-one (4.59 mg'g-1 DW) is promoted to gain the highest yield, compared to other carbon sources tested. The addition of 2.0-mg'L-1 dicamba also increases 9-methoxycanthin-6-one production (12.3 mg'g-1 DW). Higher production of 9-methoxycanthin-6-one was obtained at pH 5.5 (1.53 mg'g-1 DW). Production of 9-methoxycanthin-6-one (2.34 mg'g-1 DW) in callus cultures is also increased when the medium is added with 1×10-1 μM phenylalanine. This study suggests that the successful production of 9-methoxycanthin-6-one in vitro cultures has a potential in large-scale production using bioreactor technology. |
| first_indexed | 2025-11-15T07:59:34Z |
| format | Article |
| id | upm-14696 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:59:34Z |
| publishDate | 2009 |
| publisher | Springer Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-146962015-11-02T23:59:11Z http://psasir.upm.edu.my/id/eprint/14696/ Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia. Rosli, N. Mahmood, Maziah Chan, K. L. Sreeramanan, S. A study was conducted to improve 9-methoxycanthin-6-one productivity (potential anti-tumour compound) from callus cultures of Eurycoma longifolia (Tongkat Ali). Several factors affecting 9-methoxycanthin-6-one production in callus cultures such as different medium compositions and physical factors were investigated and analyzed. Results show that a higher production of 9-methoxycanthin-6-one (3.84 mg'g-1 DW (Dry Weight)) is obtained from callus cultured in ¼ MS basal media. At fructose of 2% (w/v), the production of 9-methoxycanthin-6-one (4.59 mg'g-1 DW) is promoted to gain the highest yield, compared to other carbon sources tested. The addition of 2.0-mg'L-1 dicamba also increases 9-methoxycanthin-6-one production (12.3 mg'g-1 DW). Higher production of 9-methoxycanthin-6-one was obtained at pH 5.5 (1.53 mg'g-1 DW). Production of 9-methoxycanthin-6-one (2.34 mg'g-1 DW) in callus cultures is also increased when the medium is added with 1×10-1 μM phenylalanine. This study suggests that the successful production of 9-methoxycanthin-6-one in vitro cultures has a potential in large-scale production using bioreactor technology. Springer Verlag 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14696/1/Factors%20affecting%20the%20accumulation%20of%209.pdf Rosli, N. and Mahmood, Maziah and Chan, K. L. and Sreeramanan, S. (2009) Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia. Journal of Forestry Research, 20 (1). pp. 54-58. ISSN 1007-662X 10.1007/s11676-009-0010-6 English |
| spellingShingle | Rosli, N. Mahmood, Maziah Chan, K. L. Sreeramanan, S. Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia. |
| title | Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia. |
| title_full | Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia. |
| title_fullStr | Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia. |
| title_full_unstemmed | Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia. |
| title_short | Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia. |
| title_sort | factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of eurycoma longifolia. |
| url | http://psasir.upm.edu.my/id/eprint/14696/ http://psasir.upm.edu.my/id/eprint/14696/ http://psasir.upm.edu.my/id/eprint/14696/1/Factors%20affecting%20the%20accumulation%20of%209.pdf |