Expression of Notch-1 receptor and its ligands Jagged-1 and Delta-1 in amoeboid microglia in postnatal rat brain and murine BV-2 cells.
Notch-1 receptor signaling pathway is involved in neuronal and glial differentiation. Its involvement in microglial functions, however, has remained elusive. This study reports the localization of Notch-1 receptor immunoreactivity in the amoeboid microglial cells (AMC) in the postnatal rat brain. By...
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| Format: | Article |
| Language: | English English |
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Wiley-Blackwell
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14673/ http://psasir.upm.edu.my/id/eprint/14673/1/Expression%20of%20Notch.pdf |
| _version_ | 1848842459250950144 |
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| author | Cao, Qiong Lu, Jia Kaur, Charanjit Sivakumar, Viswanathan Li, Fan Cheah , Pike See Dheen, S. Thameem Ling, Eng Ang |
| author_facet | Cao, Qiong Lu, Jia Kaur, Charanjit Sivakumar, Viswanathan Li, Fan Cheah , Pike See Dheen, S. Thameem Ling, Eng Ang |
| author_sort | Cao, Qiong |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Notch-1 receptor signaling pathway is involved in neuronal and glial differentiation. Its involvement in microglial functions, however, has remained elusive. This study reports the localization of Notch-1 receptor immunoreactivity in the amoeboid microglial cells (AMC) in the postnatal rat brain. By immunofluorescence, Notch-1 receptor was colocalized with its ligands, Jagged-1 and Delta-1, in the AMC in the corpus callosum and subventricular zone. Notch-1 immunopositive cells were confirmed to be microglia labeled by OX42 and lectin. Immunoexpression of Notch-1 receptor was progressively reduced with age. Western blot analysis showed that Notch-1 protein level in the corpus callosum in which the AMC were heavily populated was concomitantly decreased. In postnatal rats challenged with lipopolysaccharide (LPS), Notch-1 receptor immunofluorescence in AMC was noticeably enhanced. Furthermore, Notch-1 protein level in the corpus callosum was increased as revealed by Western blotting analysis. In primary microglial culture treated with LPS, mRNA expression of Notch-1 and its ligand Jagged-1 was upregulated but that of Delta-1 was reduced. The expression pattern of Notch-1 and its ligands was confirmed in murine BV-2 cells. Furthermore, Notch-1 neutralization with its antibody reduced its protein expression. More importantly, neutralization of Notch-1 concomitantly suppressed the mRNA expression of IL-6, IL-1, M-CSF, and iNOS; TNF-α, mRNA expression, however, was enhanced. Western blot confirmed the changes of protein level of the above except for IL-6, which remained relatively unaltered. It is concluded that Notch-1 signaling in the AMC and LPS-activated microglia/BV-2 cells modulates the expression of proinflammatory cytokines and nitric oxide. |
| first_indexed | 2025-11-15T07:59:28Z |
| format | Article |
| id | upm-14673 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:59:28Z |
| publishDate | 2008 |
| publisher | Wiley-Blackwell |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-146732015-11-23T04:15:50Z http://psasir.upm.edu.my/id/eprint/14673/ Expression of Notch-1 receptor and its ligands Jagged-1 and Delta-1 in amoeboid microglia in postnatal rat brain and murine BV-2 cells. Cao, Qiong Lu, Jia Kaur, Charanjit Sivakumar, Viswanathan Li, Fan Cheah , Pike See Dheen, S. Thameem Ling, Eng Ang Notch-1 receptor signaling pathway is involved in neuronal and glial differentiation. Its involvement in microglial functions, however, has remained elusive. This study reports the localization of Notch-1 receptor immunoreactivity in the amoeboid microglial cells (AMC) in the postnatal rat brain. By immunofluorescence, Notch-1 receptor was colocalized with its ligands, Jagged-1 and Delta-1, in the AMC in the corpus callosum and subventricular zone. Notch-1 immunopositive cells were confirmed to be microglia labeled by OX42 and lectin. Immunoexpression of Notch-1 receptor was progressively reduced with age. Western blot analysis showed that Notch-1 protein level in the corpus callosum in which the AMC were heavily populated was concomitantly decreased. In postnatal rats challenged with lipopolysaccharide (LPS), Notch-1 receptor immunofluorescence in AMC was noticeably enhanced. Furthermore, Notch-1 protein level in the corpus callosum was increased as revealed by Western blotting analysis. In primary microglial culture treated with LPS, mRNA expression of Notch-1 and its ligand Jagged-1 was upregulated but that of Delta-1 was reduced. The expression pattern of Notch-1 and its ligands was confirmed in murine BV-2 cells. Furthermore, Notch-1 neutralization with its antibody reduced its protein expression. More importantly, neutralization of Notch-1 concomitantly suppressed the mRNA expression of IL-6, IL-1, M-CSF, and iNOS; TNF-α, mRNA expression, however, was enhanced. Western blot confirmed the changes of protein level of the above except for IL-6, which remained relatively unaltered. It is concluded that Notch-1 signaling in the AMC and LPS-activated microglia/BV-2 cells modulates the expression of proinflammatory cytokines and nitric oxide. Wiley-Blackwell 2008-08-15 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14673/1/Expression%20of%20Notch.pdf Cao, Qiong and Lu, Jia and Kaur, Charanjit and Sivakumar, Viswanathan and Li, Fan and Cheah , Pike See and Dheen, S. Thameem and Ling, Eng Ang (2008) Expression of Notch-1 receptor and its ligands Jagged-1 and Delta-1 in amoeboid microglia in postnatal rat brain and murine BV-2 cells. Glia, 56 (11). pp. 1224-1237. ISSN 0894-1491; ESSN: 1098-1136 10.1002/glia.20692 English |
| spellingShingle | Cao, Qiong Lu, Jia Kaur, Charanjit Sivakumar, Viswanathan Li, Fan Cheah , Pike See Dheen, S. Thameem Ling, Eng Ang Expression of Notch-1 receptor and its ligands Jagged-1 and Delta-1 in amoeboid microglia in postnatal rat brain and murine BV-2 cells. |
| title | Expression of Notch-1 receptor and its ligands Jagged-1 and Delta-1 in amoeboid microglia in postnatal rat brain and murine BV-2 cells. |
| title_full | Expression of Notch-1 receptor and its ligands Jagged-1 and Delta-1 in amoeboid microglia in postnatal rat brain and murine BV-2 cells. |
| title_fullStr | Expression of Notch-1 receptor and its ligands Jagged-1 and Delta-1 in amoeboid microglia in postnatal rat brain and murine BV-2 cells. |
| title_full_unstemmed | Expression of Notch-1 receptor and its ligands Jagged-1 and Delta-1 in amoeboid microglia in postnatal rat brain and murine BV-2 cells. |
| title_short | Expression of Notch-1 receptor and its ligands Jagged-1 and Delta-1 in amoeboid microglia in postnatal rat brain and murine BV-2 cells. |
| title_sort | expression of notch-1 receptor and its ligands jagged-1 and delta-1 in amoeboid microglia in postnatal rat brain and murine bv-2 cells. |
| url | http://psasir.upm.edu.my/id/eprint/14673/ http://psasir.upm.edu.my/id/eprint/14673/ http://psasir.upm.edu.my/id/eprint/14673/1/Expression%20of%20Notch.pdf |