Expression analysis of adherence-associated genes in pneumococcal clinical isolates during adherence to human respiratory epithelial cells (in vitro) by real-time PCR.
Pneumococcal virulence determinants have been extensively studied but molecular evidence on virulence gene expression pattern is still lacking. We undertook this study to analyze the regulation pattern of adherence-associated genes; psaA, pspC, cbpG, including ply of serotypes 1, 7F, 19F and 23F cli...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Blackwell Publishing Ltd.
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14672/ http://psasir.upm.edu.my/id/eprint/14672/1/Expression%20analysis%20of%20adherence.pdf |
| _version_ | 1848842459003486208 |
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| author | Mohd Desa, Mohd Nasir Navaratnam, Parasakthi Vadivelu, Jamunarani Sekaran, Shamala Devi |
| author_facet | Mohd Desa, Mohd Nasir Navaratnam, Parasakthi Vadivelu, Jamunarani Sekaran, Shamala Devi |
| author_sort | Mohd Desa, Mohd Nasir |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Pneumococcal virulence determinants have been extensively studied but molecular evidence on virulence gene expression pattern is still lacking. We undertook this study to analyze the regulation pattern of adherence-associated genes; psaA, pspC, cbpG, including ply of serotypes 1, 7F, 19F and 23F clinical isolates during the bacterial adherence to human lung epithelial cells (in vitro), by real-time PCR. We were able to harvest the bacterial RNA (0.5-1 μg μL-1) from the infected host cell and analysis showed a consistent upregulation of psaA. Differential expressions were observed for pspC, cbpG and ply genes but the former was mostly upregulated whereas the later two frequently showed either no significant change or a downregulation. Partial nucleotide sequences of psaA, cbpG and ply were highly homologous among the isolates as well as against GenBank sequences (99%) whereas those for pspC were similar (98%) to allelic variants pspC-3 and pspC-5. |
| first_indexed | 2025-11-15T07:59:28Z |
| format | Article |
| id | upm-14672 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:59:28Z |
| publishDate | 2008 |
| publisher | Blackwell Publishing Ltd. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-146722015-10-23T06:39:02Z http://psasir.upm.edu.my/id/eprint/14672/ Expression analysis of adherence-associated genes in pneumococcal clinical isolates during adherence to human respiratory epithelial cells (in vitro) by real-time PCR. Mohd Desa, Mohd Nasir Navaratnam, Parasakthi Vadivelu, Jamunarani Sekaran, Shamala Devi Pneumococcal virulence determinants have been extensively studied but molecular evidence on virulence gene expression pattern is still lacking. We undertook this study to analyze the regulation pattern of adherence-associated genes; psaA, pspC, cbpG, including ply of serotypes 1, 7F, 19F and 23F clinical isolates during the bacterial adherence to human lung epithelial cells (in vitro), by real-time PCR. We were able to harvest the bacterial RNA (0.5-1 μg μL-1) from the infected host cell and analysis showed a consistent upregulation of psaA. Differential expressions were observed for pspC, cbpG and ply genes but the former was mostly upregulated whereas the later two frequently showed either no significant change or a downregulation. Partial nucleotide sequences of psaA, cbpG and ply were highly homologous among the isolates as well as against GenBank sequences (99%) whereas those for pspC were similar (98%) to allelic variants pspC-3 and pspC-5. Blackwell Publishing Ltd. 2008-11 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14672/1/Expression%20analysis%20of%20adherence.pdf Mohd Desa, Mohd Nasir and Navaratnam, Parasakthi and Vadivelu, Jamunarani and Sekaran, Shamala Devi (2008) Expression analysis of adherence-associated genes in pneumococcal clinical isolates during adherence to human respiratory epithelial cells (in vitro) by real-time PCR. FEMS Microbiology Letters, 288 (1). pp. 125-130. ISSN 0378-1097; ESSN: 1574-6968 10.1111/j.1574-6968.2008.01345.x English |
| spellingShingle | Mohd Desa, Mohd Nasir Navaratnam, Parasakthi Vadivelu, Jamunarani Sekaran, Shamala Devi Expression analysis of adherence-associated genes in pneumococcal clinical isolates during adherence to human respiratory epithelial cells (in vitro) by real-time PCR. |
| title | Expression analysis of adherence-associated genes in pneumococcal clinical isolates during adherence to human respiratory epithelial cells (in vitro) by real-time PCR. |
| title_full | Expression analysis of adherence-associated genes in pneumococcal clinical isolates during adherence to human respiratory epithelial cells (in vitro) by real-time PCR. |
| title_fullStr | Expression analysis of adherence-associated genes in pneumococcal clinical isolates during adherence to human respiratory epithelial cells (in vitro) by real-time PCR. |
| title_full_unstemmed | Expression analysis of adherence-associated genes in pneumococcal clinical isolates during adherence to human respiratory epithelial cells (in vitro) by real-time PCR. |
| title_short | Expression analysis of adherence-associated genes in pneumococcal clinical isolates during adherence to human respiratory epithelial cells (in vitro) by real-time PCR. |
| title_sort | expression analysis of adherence-associated genes in pneumococcal clinical isolates during adherence to human respiratory epithelial cells (in vitro) by real-time pcr. |
| url | http://psasir.upm.edu.my/id/eprint/14672/ http://psasir.upm.edu.my/id/eprint/14672/ http://psasir.upm.edu.my/id/eprint/14672/1/Expression%20analysis%20of%20adherence.pdf |