Exploring SOD1 gene for the detection of fetal Down syndrome

Objective: Fetal cells and circulating cellfree fetal DNA increases in the maternal circulation in women carrying trisomy 21 fetus. Methods: We attempted the use of superoxide dismutase (SOD-1) gene, which is located at the Down Syndrome Critical Region, to overcome this situation for the prena...

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Main Authors: Karrupiah, Thilakavathy, Rosli, Rozita, Baskaran, T. P.
Format: Article
Language:English
Published: CIC Edizioni Internazionali 2008
Online Access:http://psasir.upm.edu.my/id/eprint/14662/
http://psasir.upm.edu.my/id/eprint/14662/1/Exploring%20SOD1%20gene%20for%20the%20detection%20of%20fetal%20down%20syndrome.pdf
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author Karrupiah, Thilakavathy
Rosli, Rozita
Baskaran, T. P.
author_facet Karrupiah, Thilakavathy
Rosli, Rozita
Baskaran, T. P.
author_sort Karrupiah, Thilakavathy
building UPM Institutional Repository
collection Online Access
description Objective: Fetal cells and circulating cellfree fetal DNA increases in the maternal circulation in women carrying trisomy 21 fetus. Methods: We attempted the use of superoxide dismutase (SOD-1) gene, which is located at the Down Syndrome Critical Region, to overcome this situation for the prenatal screening of Down syndrome. The prospective of the gene using real-time quantitative polymerase chain reaction was explored. Results: The level of SOD-1 sequences is significantly elevated in the third trimester normal pregnancies (mean = 11728 copies/μl) when compared to the second trimester (mean = 5705.6 copies/μl), (p<0.005) and non pregnant normal women (mean = 3580.2 copies/μl), (p<0.0001). Down syndrome pregnancies have the greatest elevation compared to all the three trimesters of normal singleton pregnancies and twin pregnancies, p<0.05. Conclusions: These data indicate that a quantitative analysis using a gene associated with a disorder could be used in screening for the prenatal diagnosis of fetal aneuploidies regardless of the sex of the fetus.
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spelling upm-146622019-11-01T03:59:40Z http://psasir.upm.edu.my/id/eprint/14662/ Exploring SOD1 gene for the detection of fetal Down syndrome Karrupiah, Thilakavathy Rosli, Rozita Baskaran, T. P. Objective: Fetal cells and circulating cellfree fetal DNA increases in the maternal circulation in women carrying trisomy 21 fetus. Methods: We attempted the use of superoxide dismutase (SOD-1) gene, which is located at the Down Syndrome Critical Region, to overcome this situation for the prenatal screening of Down syndrome. The prospective of the gene using real-time quantitative polymerase chain reaction was explored. Results: The level of SOD-1 sequences is significantly elevated in the third trimester normal pregnancies (mean = 11728 copies/μl) when compared to the second trimester (mean = 5705.6 copies/μl), (p<0.005) and non pregnant normal women (mean = 3580.2 copies/μl), (p<0.0001). Down syndrome pregnancies have the greatest elevation compared to all the three trimesters of normal singleton pregnancies and twin pregnancies, p<0.05. Conclusions: These data indicate that a quantitative analysis using a gene associated with a disorder could be used in screening for the prenatal diagnosis of fetal aneuploidies regardless of the sex of the fetus. CIC Edizioni Internazionali 2008-09 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14662/1/Exploring%20SOD1%20gene%20for%20the%20detection%20of%20fetal%20down%20syndrome.pdf Karrupiah, Thilakavathy and Rosli, Rozita and Baskaran, T. P. (2008) Exploring SOD1 gene for the detection of fetal Down syndrome. Journal of Prenatal Medicine, 2 (3). pp. 36-39. ISSN 1971-3282; ESSN: 1971-3290 https://www.prenatalmedicine.com/index.php?PAGE=articolo_dett&ID_ISSUE=391&id_article=3390
spellingShingle Karrupiah, Thilakavathy
Rosli, Rozita
Baskaran, T. P.
Exploring SOD1 gene for the detection of fetal Down syndrome
title Exploring SOD1 gene for the detection of fetal Down syndrome
title_full Exploring SOD1 gene for the detection of fetal Down syndrome
title_fullStr Exploring SOD1 gene for the detection of fetal Down syndrome
title_full_unstemmed Exploring SOD1 gene for the detection of fetal Down syndrome
title_short Exploring SOD1 gene for the detection of fetal Down syndrome
title_sort exploring sod1 gene for the detection of fetal down syndrome
url http://psasir.upm.edu.my/id/eprint/14662/
http://psasir.upm.edu.my/id/eprint/14662/
http://psasir.upm.edu.my/id/eprint/14662/1/Exploring%20SOD1%20gene%20for%20the%20detection%20of%20fetal%20down%20syndrome.pdf