Evaluation of in vitro Vibrio static activity of Shewanella algae isolated from healthy Penaeus monodon

To conquer disease problem in shrimp industries, probiotic biocontrol is a well known remedy now. The antagonistic ability of separated isolates from different parts of juvenile Penaeus monodon were screened against shrimp Vibrio pathogens; Vibrio parahaemolyticus and Vibrio alginolyticus. The most...

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Main Authors: Shakibazadeh, Shahram, Saad, Che Roos, Christianus, Annie, Kamarudin, Mohd Salleh, Sijam, Kamaruzaman, Shamsudin, Mariana Nor, Neela, Vasantha Kumari
Format: Article
Language:English
Published: Academic Journals 2008
Online Access:http://psasir.upm.edu.my/id/eprint/14603/
http://psasir.upm.edu.my/id/eprint/14603/1/14603.pdf
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author Shakibazadeh, Shahram
Saad, Che Roos
Christianus, Annie
Kamarudin, Mohd Salleh
Sijam, Kamaruzaman
Shamsudin, Mariana Nor
Neela, Vasantha Kumari
author_facet Shakibazadeh, Shahram
Saad, Che Roos
Christianus, Annie
Kamarudin, Mohd Salleh
Sijam, Kamaruzaman
Shamsudin, Mariana Nor
Neela, Vasantha Kumari
author_sort Shakibazadeh, Shahram
building UPM Institutional Repository
collection Online Access
description To conquer disease problem in shrimp industries, probiotic biocontrol is a well known remedy now. The antagonistic ability of separated isolates from different parts of juvenile Penaeus monodon were screened against shrimp Vibrio pathogens; Vibrio parahaemolyticus and Vibrio alginolyticus. The most antagonistic effect was observed for an isolate that was primarily identified as Shewanella algae using conventional methods followed by Biolog microlog software. Since production of antagonistic agents rely on cultural conditions, antagonistic ability of candidate probioic against the mentioned Vibrios was assessed using Response Surface Methodology, with central composite design in which four independents variables were assumed: temperature (10 - 50°C), pH (6 -10), NaCl concentration (0 - 50%) and time (12 – 60 h). The coefficients of multiple determinations (R2), for the responses of antagonistic effect of S. algae against V. parahaemolyticus and V. alginolyticus values were 0.807 and 0.805, respectively. Concentration of the NaCl exhibited least influence on the antibacterial effect of candidate probiotic while the other independent variables exhibited different degree of affect. The candidate probiotic revealed a reasonable antibacterial response in quite a wide range of temperature and pH in which the maximum levels were in the same range of optimum shrimp culture.
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spelling upm-146032016-05-31T05:21:16Z http://psasir.upm.edu.my/id/eprint/14603/ Evaluation of in vitro Vibrio static activity of Shewanella algae isolated from healthy Penaeus monodon Shakibazadeh, Shahram Saad, Che Roos Christianus, Annie Kamarudin, Mohd Salleh Sijam, Kamaruzaman Shamsudin, Mariana Nor Neela, Vasantha Kumari To conquer disease problem in shrimp industries, probiotic biocontrol is a well known remedy now. The antagonistic ability of separated isolates from different parts of juvenile Penaeus monodon were screened against shrimp Vibrio pathogens; Vibrio parahaemolyticus and Vibrio alginolyticus. The most antagonistic effect was observed for an isolate that was primarily identified as Shewanella algae using conventional methods followed by Biolog microlog software. Since production of antagonistic agents rely on cultural conditions, antagonistic ability of candidate probioic against the mentioned Vibrios was assessed using Response Surface Methodology, with central composite design in which four independents variables were assumed: temperature (10 - 50°C), pH (6 -10), NaCl concentration (0 - 50%) and time (12 – 60 h). The coefficients of multiple determinations (R2), for the responses of antagonistic effect of S. algae against V. parahaemolyticus and V. alginolyticus values were 0.807 and 0.805, respectively. Concentration of the NaCl exhibited least influence on the antibacterial effect of candidate probiotic while the other independent variables exhibited different degree of affect. The candidate probiotic revealed a reasonable antibacterial response in quite a wide range of temperature and pH in which the maximum levels were in the same range of optimum shrimp culture. Academic Journals 2008-11-05 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14603/1/14603.pdf Shakibazadeh, Shahram and Saad, Che Roos and Christianus, Annie and Kamarudin, Mohd Salleh and Sijam, Kamaruzaman and Shamsudin, Mariana Nor and Neela, Vasantha Kumari (2008) Evaluation of in vitro Vibrio static activity of Shewanella algae isolated from healthy Penaeus monodon. African Journal of Biotechnology, 7 (21). art. no. 84D09E97976. pp. 3952-3961. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/84D09E97976 10.5897/AJB08.799
spellingShingle Shakibazadeh, Shahram
Saad, Che Roos
Christianus, Annie
Kamarudin, Mohd Salleh
Sijam, Kamaruzaman
Shamsudin, Mariana Nor
Neela, Vasantha Kumari
Evaluation of in vitro Vibrio static activity of Shewanella algae isolated from healthy Penaeus monodon
title Evaluation of in vitro Vibrio static activity of Shewanella algae isolated from healthy Penaeus monodon
title_full Evaluation of in vitro Vibrio static activity of Shewanella algae isolated from healthy Penaeus monodon
title_fullStr Evaluation of in vitro Vibrio static activity of Shewanella algae isolated from healthy Penaeus monodon
title_full_unstemmed Evaluation of in vitro Vibrio static activity of Shewanella algae isolated from healthy Penaeus monodon
title_short Evaluation of in vitro Vibrio static activity of Shewanella algae isolated from healthy Penaeus monodon
title_sort evaluation of in vitro vibrio static activity of shewanella algae isolated from healthy penaeus monodon
url http://psasir.upm.edu.my/id/eprint/14603/
http://psasir.upm.edu.my/id/eprint/14603/
http://psasir.upm.edu.my/id/eprint/14603/
http://psasir.upm.edu.my/id/eprint/14603/1/14603.pdf