Epicatechin content and antioxidant capacity of cocoa beans from four different countries

Natural antioxidant has received more attention to be part of daily diet. Cocoa beans is one of the main sources of polyphenols especially epicatechin. This study was conducted to investigate the relationship between antioxidant potential and epicatechin content of raw cocoa beans from different cou...

Full description

Bibliographic Details
Main Authors: Othman, Azizah, Mhd Jalil, Abbe Maleyki, Kong, Kin Weng, Ismail, Amin, Abd. Ghani, Nawalyah, Adenan, Mohd Ilham
Format: Article
Language:English
Published: Academic Journals 2010
Online Access:http://psasir.upm.edu.my/id/eprint/14544/
http://psasir.upm.edu.my/id/eprint/14544/1/14544.pdf
_version_ 1848842423245996032
author Othman, Azizah
Mhd Jalil, Abbe Maleyki
Kong, Kin Weng
Ismail, Amin
Abd. Ghani, Nawalyah
Adenan, Mohd Ilham
author_facet Othman, Azizah
Mhd Jalil, Abbe Maleyki
Kong, Kin Weng
Ismail, Amin
Abd. Ghani, Nawalyah
Adenan, Mohd Ilham
author_sort Othman, Azizah
building UPM Institutional Repository
collection Online Access
description Natural antioxidant has received more attention to be part of daily diet. Cocoa beans is one of the main sources of polyphenols especially epicatechin. This study was conducted to investigate the relationship between antioxidant potential and epicatechin content of raw cocoa beans from different countries, namely Malaysia, Ghana, Cote d'Ivoire and Sulawesi (Indonesia). Antioxidant potential was determined using trolox-equivalent antioxidant capacity (TEAC) and ferric reducing antioxidant power (FRAP) assays. Reversed-phase high performance liquid chromatography (HPLC) was used to quantify the amount of epicatechin. The epicatechin content of raw cocoa beans was in the range of 270 - 1235 mg/100 g cocoa beans. Based on the two assays, Sulawesian beans exhibited the highest antioxidant capacity followed by Malaysian, Ghanaian and Cote d'Ivoirian beans for both extracts. Both ethanolic (r = 0.92) and water (r = 0.90) extracts of cocoa beans showed a significant positive and high correlation between epicatechin and TEAC value. Similarly, FRAP assay also showed a positive and high correlation with epicatechin for both ethanolic (r = 0.84) and water (r = 0.79) extracts. Results indicated that antioxidant capacity using two different antioxidant assays exhibited a positive and high correlation with epicatechin content in cocoa beans. Thus, epicatechin content in cocoa beans could be responsible for the antioxidant capacity.
first_indexed 2025-11-15T07:58:54Z
format Article
id upm-14544
institution Universiti Putra Malaysia
institution_category Local University
language English
last_indexed 2025-11-15T07:58:54Z
publishDate 2010
publisher Academic Journals
recordtype eprints
repository_type Digital Repository
spelling upm-145442017-05-08T02:21:42Z http://psasir.upm.edu.my/id/eprint/14544/ Epicatechin content and antioxidant capacity of cocoa beans from four different countries Othman, Azizah Mhd Jalil, Abbe Maleyki Kong, Kin Weng Ismail, Amin Abd. Ghani, Nawalyah Adenan, Mohd Ilham Natural antioxidant has received more attention to be part of daily diet. Cocoa beans is one of the main sources of polyphenols especially epicatechin. This study was conducted to investigate the relationship between antioxidant potential and epicatechin content of raw cocoa beans from different countries, namely Malaysia, Ghana, Cote d'Ivoire and Sulawesi (Indonesia). Antioxidant potential was determined using trolox-equivalent antioxidant capacity (TEAC) and ferric reducing antioxidant power (FRAP) assays. Reversed-phase high performance liquid chromatography (HPLC) was used to quantify the amount of epicatechin. The epicatechin content of raw cocoa beans was in the range of 270 - 1235 mg/100 g cocoa beans. Based on the two assays, Sulawesian beans exhibited the highest antioxidant capacity followed by Malaysian, Ghanaian and Cote d'Ivoirian beans for both extracts. Both ethanolic (r = 0.92) and water (r = 0.90) extracts of cocoa beans showed a significant positive and high correlation between epicatechin and TEAC value. Similarly, FRAP assay also showed a positive and high correlation with epicatechin for both ethanolic (r = 0.84) and water (r = 0.79) extracts. Results indicated that antioxidant capacity using two different antioxidant assays exhibited a positive and high correlation with epicatechin content in cocoa beans. Thus, epicatechin content in cocoa beans could be responsible for the antioxidant capacity. Academic Journals 2010-02-15 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14544/1/14544.pdf Othman, Azizah and Mhd Jalil, Abbe Maleyki and Kong, Kin Weng and Ismail, Amin and Abd. Ghani, Nawalyah and Adenan, Mohd Ilham (2010) Epicatechin content and antioxidant capacity of cocoa beans from four different countries. African Journal of Biotechnology, 9 (7). art. no. B71D7A736022. pp. 1052-1059. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/B71D7A736022
spellingShingle Othman, Azizah
Mhd Jalil, Abbe Maleyki
Kong, Kin Weng
Ismail, Amin
Abd. Ghani, Nawalyah
Adenan, Mohd Ilham
Epicatechin content and antioxidant capacity of cocoa beans from four different countries
title Epicatechin content and antioxidant capacity of cocoa beans from four different countries
title_full Epicatechin content and antioxidant capacity of cocoa beans from four different countries
title_fullStr Epicatechin content and antioxidant capacity of cocoa beans from four different countries
title_full_unstemmed Epicatechin content and antioxidant capacity of cocoa beans from four different countries
title_short Epicatechin content and antioxidant capacity of cocoa beans from four different countries
title_sort epicatechin content and antioxidant capacity of cocoa beans from four different countries
url http://psasir.upm.edu.my/id/eprint/14544/
http://psasir.upm.edu.my/id/eprint/14544/
http://psasir.upm.edu.my/id/eprint/14544/1/14544.pdf