Enzyme‐assisted aqueous extraction of Kalahari melon seed oil: optimization using response surface methodology
Enzymatic extraction of oil from Kalahari melon seeds was investigated and evaluated by response surface methodology (RSM). Two commercial protease enzyme products were used separately: Neutrase® 0.8 L and Flavourzyme® 1000 L from Novozymes (Bagsvaerd, Denmark). RSM was applied to model and optimize...
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| Format: | Article |
| Language: | English |
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American Oil Chemists' Society
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14543/ http://psasir.upm.edu.my/id/eprint/14543/1/14543.pdf |
| _version_ | 1848842422934568960 |
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| author | Nyam, Kar Lin Tan, Chin Ping Lai, Oi Ming Long, Kamariah Che Man, Yaakob |
| author_facet | Nyam, Kar Lin Tan, Chin Ping Lai, Oi Ming Long, Kamariah Che Man, Yaakob |
| author_sort | Nyam, Kar Lin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Enzymatic extraction of oil from Kalahari melon seeds was investigated and evaluated by response surface methodology (RSM). Two commercial protease enzyme products were used separately: Neutrase® 0.8 L and Flavourzyme® 1000 L from Novozymes (Bagsvaerd, Denmark). RSM was applied to model and optimize the reaction conditions namely concentration of enzyme (20–50 g kg−1 of seed mass), initial pH of mixture (pH 5–9), incubation temperature (40–60 °C), and incubation time (12–36 h). Well fitting models were successfully established for both enzymes: Neutrase 0.8 L (R 2 = 0.9410) and Flavourzyme 1000 L (R 2 = 0.9574) through multiple linear regressions with backward elimination. Incubation time was the most significant reaction factor on oil yield for both enzymes. The optimal conditions for Neutrase 0.8 L were: an enzyme concentration of 25 g kg−1, an initial pH of 7, a temperature at 58 °C and an incubation time of 31 h with constant shaking at 100 rpm. Centrifuging the mixture at 8,000g for 20 min separated the oil with a recovery of 68.58 ± 3.39%. The optimal conditions for Flavourzyme 1000 L were enzyme concentration of 21 g kg−1, initial pH of 6, temperature at 50 °C and incubation time of 36 h. These optimum conditions yielded a 71.55 ± 1.28% oil recovery. |
| first_indexed | 2025-11-15T07:58:53Z |
| format | Article |
| id | upm-14543 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:58:53Z |
| publishDate | 2009 |
| publisher | American Oil Chemists' Society |
| recordtype | eprints |
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| spelling | upm-145432020-07-17T02:14:20Z http://psasir.upm.edu.my/id/eprint/14543/ Enzyme‐assisted aqueous extraction of Kalahari melon seed oil: optimization using response surface methodology Nyam, Kar Lin Tan, Chin Ping Lai, Oi Ming Long, Kamariah Che Man, Yaakob Enzymatic extraction of oil from Kalahari melon seeds was investigated and evaluated by response surface methodology (RSM). Two commercial protease enzyme products were used separately: Neutrase® 0.8 L and Flavourzyme® 1000 L from Novozymes (Bagsvaerd, Denmark). RSM was applied to model and optimize the reaction conditions namely concentration of enzyme (20–50 g kg−1 of seed mass), initial pH of mixture (pH 5–9), incubation temperature (40–60 °C), and incubation time (12–36 h). Well fitting models were successfully established for both enzymes: Neutrase 0.8 L (R 2 = 0.9410) and Flavourzyme 1000 L (R 2 = 0.9574) through multiple linear regressions with backward elimination. Incubation time was the most significant reaction factor on oil yield for both enzymes. The optimal conditions for Neutrase 0.8 L were: an enzyme concentration of 25 g kg−1, an initial pH of 7, a temperature at 58 °C and an incubation time of 31 h with constant shaking at 100 rpm. Centrifuging the mixture at 8,000g for 20 min separated the oil with a recovery of 68.58 ± 3.39%. The optimal conditions for Flavourzyme 1000 L were enzyme concentration of 21 g kg−1, initial pH of 6, temperature at 50 °C and incubation time of 36 h. These optimum conditions yielded a 71.55 ± 1.28% oil recovery. American Oil Chemists' Society 2009 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/14543/1/14543.pdf Nyam, Kar Lin and Tan, Chin Ping and Lai, Oi Ming and Long, Kamariah and Che Man, Yaakob (2009) Enzyme‐assisted aqueous extraction of Kalahari melon seed oil: optimization using response surface methodology. Journal of the American Oil Chemists' Society, 86 (12). pp. 1235-1240. ISSN 0003-021X; ESSN: 1558-9331 https://aocs.onlinelibrary.wiley.com/doi/full/10.1007/s11746-009-1462-8 10.1007/s11746-009-1462-8 |
| spellingShingle | Nyam, Kar Lin Tan, Chin Ping Lai, Oi Ming Long, Kamariah Che Man, Yaakob Enzyme‐assisted aqueous extraction of Kalahari melon seed oil: optimization using response surface methodology |
| title | Enzyme‐assisted aqueous extraction of Kalahari melon seed oil: optimization using response surface methodology |
| title_full | Enzyme‐assisted aqueous extraction of Kalahari melon seed oil: optimization using response surface methodology |
| title_fullStr | Enzyme‐assisted aqueous extraction of Kalahari melon seed oil: optimization using response surface methodology |
| title_full_unstemmed | Enzyme‐assisted aqueous extraction of Kalahari melon seed oil: optimization using response surface methodology |
| title_short | Enzyme‐assisted aqueous extraction of Kalahari melon seed oil: optimization using response surface methodology |
| title_sort | enzyme‐assisted aqueous extraction of kalahari melon seed oil: optimization using response surface methodology |
| url | http://psasir.upm.edu.my/id/eprint/14543/ http://psasir.upm.edu.my/id/eprint/14543/ http://psasir.upm.edu.my/id/eprint/14543/ http://psasir.upm.edu.my/id/eprint/14543/1/14543.pdf |