Enzymatic synthesis and characterization of palm-based kojic acid ester
Kojic acid ester was synthesized from acyl donor (fatty acid/palm oil) and kojic acid by esterification using lipase as a biocatalyst in an organic medium. Analysis of the product using GC and FTIR showed the presence of kojic acid ester. The gas chromatography-mass spectrometry (GC-MS) analysis a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Malaysian Palm Oil Board
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14539/ http://psasir.upm.edu.my/id/eprint/14539/1/Enzymatic%20synthesis%20and%20characterization%20of%20palm.pdf |
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| author | Khamaruddin, Noor Hayati Basri, Mahiran Ee, Gwendoline Cheng Lian Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Ariff, Arbakariya Mohamad, Rosfarizan Awang, Roila |
| author_facet | Khamaruddin, Noor Hayati Basri, Mahiran Ee, Gwendoline Cheng Lian Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Ariff, Arbakariya Mohamad, Rosfarizan Awang, Roila |
| author_sort | Khamaruddin, Noor Hayati |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Kojic acid ester was synthesized from acyl donor (fatty acid/palm oil) and kojic acid by esterification using
lipase as a biocatalyst in an organic medium. Analysis of the product using GC and FTIR showed the presence
of kojic acid ester. The gas chromatography-mass spectrometry (GC-MS) analysis and 1H-NMR and 13C-NMR spectral data confirmed the molecular structure of the kojic acid ester. Among the enzymes tested,lipase from
Pseudomonas cepacia gave the highest synthetic specific activity. Oleic acid was found to be the best substrate with which to produce the ester in acetonitrile. The optimum conditions for the synthesis of kojic acid derivatives using Pseudomonas cepacia lipase were time, 24 hr, temperature, 50oC, amount of enzyme, 0.15 g, solvent of log P = -0.33, mole ratio of 4 (kojic acid/oleic acid), with no added water, no control of water activity and oleic acid as substrate. |
| first_indexed | 2025-11-15T07:58:52Z |
| format | Article |
| id | upm-14539 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:58:52Z |
| publishDate | 2008 |
| publisher | Malaysian Palm Oil Board |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-145392016-09-28T02:02:05Z http://psasir.upm.edu.my/id/eprint/14539/ Enzymatic synthesis and characterization of palm-based kojic acid ester Khamaruddin, Noor Hayati Basri, Mahiran Ee, Gwendoline Cheng Lian Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Ariff, Arbakariya Mohamad, Rosfarizan Awang, Roila Kojic acid ester was synthesized from acyl donor (fatty acid/palm oil) and kojic acid by esterification using lipase as a biocatalyst in an organic medium. Analysis of the product using GC and FTIR showed the presence of kojic acid ester. The gas chromatography-mass spectrometry (GC-MS) analysis and 1H-NMR and 13C-NMR spectral data confirmed the molecular structure of the kojic acid ester. Among the enzymes tested,lipase from Pseudomonas cepacia gave the highest synthetic specific activity. Oleic acid was found to be the best substrate with which to produce the ester in acetonitrile. The optimum conditions for the synthesis of kojic acid derivatives using Pseudomonas cepacia lipase were time, 24 hr, temperature, 50oC, amount of enzyme, 0.15 g, solvent of log P = -0.33, mole ratio of 4 (kojic acid/oleic acid), with no added water, no control of water activity and oleic acid as substrate. Malaysian Palm Oil Board 2008-06 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14539/1/Enzymatic%20synthesis%20and%20characterization%20of%20palm.pdf Khamaruddin, Noor Hayati and Basri, Mahiran and Ee, Gwendoline Cheng Lian and Salleh, Abu Bakar and Raja Abdul Rahman, Raja Noor Zaliha and Ariff, Arbakariya and Mohamad, Rosfarizan and Awang, Roila (2008) Enzymatic synthesis and characterization of palm-based kojic acid ester. Journal of Oil Palm Research, 20. pp. 461-469. ISSN 1511-2780 http://jopr.mpob.gov.my/enzymatic-synthesis-and-characterization-of-palm-based-kojic-acid-ester/ |
| spellingShingle | Khamaruddin, Noor Hayati Basri, Mahiran Ee, Gwendoline Cheng Lian Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Ariff, Arbakariya Mohamad, Rosfarizan Awang, Roila Enzymatic synthesis and characterization of palm-based kojic acid ester |
| title | Enzymatic synthesis and characterization of palm-based kojic acid ester |
| title_full | Enzymatic synthesis and characterization of palm-based kojic acid ester |
| title_fullStr | Enzymatic synthesis and characterization of palm-based kojic acid ester |
| title_full_unstemmed | Enzymatic synthesis and characterization of palm-based kojic acid ester |
| title_short | Enzymatic synthesis and characterization of palm-based kojic acid ester |
| title_sort | enzymatic synthesis and characterization of palm-based kojic acid ester |
| url | http://psasir.upm.edu.my/id/eprint/14539/ http://psasir.upm.edu.my/id/eprint/14539/ http://psasir.upm.edu.my/id/eprint/14539/1/Enzymatic%20synthesis%20and%20characterization%20of%20palm.pdf |