Enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells
BACKGROUND: Many reports have suggested that mild hypothermic culture conditions improve the specific monoclonal antibody (mAb) productivity of mammalian cells. The effect of active hypothermic growth on the mAb productivity of the hybridoma C2E7 was investigated. Hybridoma growth under hypothermic...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Society of Chemical Industry
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14517/ http://psasir.upm.edu.my/id/eprint/14517/1/Enhancement%20of%20monoclonal%20antibody%20productivity%20by%20promoting%20active%20hypothermic%20growth%20in%20hybridoma%20cells.pdf |
| _version_ | 1848842415619702784 |
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| author | Chong, Sun Li Mou, Duen Gang Lim, Saw Hoon Ali, Abdul Manaf Tey, Beng Ti |
| author_facet | Chong, Sun Li Mou, Duen Gang Lim, Saw Hoon Ali, Abdul Manaf Tey, Beng Ti |
| author_sort | Chong, Sun Li |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | BACKGROUND: Many reports have suggested that mild hypothermic culture conditions improve the specific monoclonal antibody (mAb) productivity of mammalian cells. The effect of active hypothermic growth on the mAb productivity of the hybridoma C2E7 was investigated. Hybridoma growth under hypothermic conditions (32 °C) was stimulated by supplementation of the culture medium with high serum concentrations (up to 30%).
RESULTS: Specific and volumetric mAb productivity of a stimulated, active growth, mildly hypothermic hybridoma culture (30% FBS supplemented, 32 °C) were 1.38‐ and 1.34‐fold greater than the control culture (10% FBS supplemented, 37 °C). The enhanced specific mAb productivity under hypothermic conditions was associated with an increase in IgM mRNA levels during both the lag and early exponential phases of hypothermic growth.
CONCLUSION: Stimulation of hybridoma growth under mildly hypothermic conditions increased both the specific and volumetric mAb productivity of hybridoma cells. |
| first_indexed | 2025-11-15T07:58:46Z |
| format | Article |
| id | upm-14517 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:58:46Z |
| publishDate | 2009 |
| publisher | Society of Chemical Industry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-145172019-04-08T08:51:07Z http://psasir.upm.edu.my/id/eprint/14517/ Enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells Chong, Sun Li Mou, Duen Gang Lim, Saw Hoon Ali, Abdul Manaf Tey, Beng Ti BACKGROUND: Many reports have suggested that mild hypothermic culture conditions improve the specific monoclonal antibody (mAb) productivity of mammalian cells. The effect of active hypothermic growth on the mAb productivity of the hybridoma C2E7 was investigated. Hybridoma growth under hypothermic conditions (32 °C) was stimulated by supplementation of the culture medium with high serum concentrations (up to 30%). RESULTS: Specific and volumetric mAb productivity of a stimulated, active growth, mildly hypothermic hybridoma culture (30% FBS supplemented, 32 °C) were 1.38‐ and 1.34‐fold greater than the control culture (10% FBS supplemented, 37 °C). The enhanced specific mAb productivity under hypothermic conditions was associated with an increase in IgM mRNA levels during both the lag and early exponential phases of hypothermic growth. CONCLUSION: Stimulation of hybridoma growth under mildly hypothermic conditions increased both the specific and volumetric mAb productivity of hybridoma cells. Society of Chemical Industry 2009 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/14517/1/Enhancement%20of%20monoclonal%20antibody%20productivity%20by%20promoting%20active%20hypothermic%20growth%20in%20hybridoma%20cells.pdf Chong, Sun Li and Mou, Duen Gang and Lim, Saw Hoon and Ali, Abdul Manaf and Tey, Beng Ti (2009) Enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells. Journal of Chemical Technology and Biotechnology, 84 (11). pp. 1674-1680. ISSN 0268-2575; ESSN: 1097-4660 https://onlinelibrary.wiley.com/doi/abs/10.1002/jctb.2228 10.1002/jctb.2228 |
| spellingShingle | Chong, Sun Li Mou, Duen Gang Lim, Saw Hoon Ali, Abdul Manaf Tey, Beng Ti Enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells |
| title | Enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells |
| title_full | Enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells |
| title_fullStr | Enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells |
| title_full_unstemmed | Enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells |
| title_short | Enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells |
| title_sort | enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells |
| url | http://psasir.upm.edu.my/id/eprint/14517/ http://psasir.upm.edu.my/id/eprint/14517/ http://psasir.upm.edu.my/id/eprint/14517/ http://psasir.upm.edu.my/id/eprint/14517/1/Enhancement%20of%20monoclonal%20antibody%20productivity%20by%20promoting%20active%20hypothermic%20growth%20in%20hybridoma%20cells.pdf |