Enhanced secretory production of hemolysin-mediated cyclodextrin-glucanotransferase in Escherichia coli by random mutagenesis of the ABC transporter system.

QR Code

Enhanced secretory production of hemolysin-mediated cyclodextrin-glucanotransferase in Escherichia coli by random mutagenesis of the ABC transporter system.

The hemolysin transport system was found to mediate the release of cyclodextrin glucanotransferase (CGTase) into the extracellular medium when it was fused to the C-terminal 61 amino acids of HlyA (HlyAs(61)). To produce an improved-secretion variant, the hly components (hlyAs, hlyB and hlyD) were e...

Full description

Bibliographic Details
Main Authors:	Kheng, Oon Low, Mahadi, Nor Muhammad, Abdul Rahim, Raha, Rabu, Amir, Abu Bakar, Farah Diba, Abdul Murad, Abdul Munir, Md. Illias, Rosli
Format:	Article
Language:	English English
Published:	Elsevier 2010
Online Access:	http://psasir.upm.edu.my/id/eprint/14512/ http://psasir.upm.edu.my/id/eprint/14512/1/Enhanced%20secretory%20production%20of%20hemolysin.pdf

Similar Items

An effective extracellular protein secretion by an ABC transporter system in Escherichia coli: statistical modeling and optimization of cyclodextrin glucanotransferase secretory production
by: Kheng, Oon Low, et al.
Published: (2011)

Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability
by: Rohaida, Che Man, et al.
Published: (2015)

Effects of the immobilization of recombinant Escherichia coli on cyclodextrin glucanotransferase (cgtase) excretion and cell viability
by: Mohd Fuzi, Siti Fatimah Zaharah, et al.
Published: (2015)

Immobilization of escherichia coli producing β-cyclodextrin glucanotransferase on hollow fiber membrane for β-cyclodextrin production
by: Nurul Nabila Huda, Baharudin
Published: (2023)

Enhanced excretion of recombinant cyclodextrin glucanotransferase and cell stability of immobilized recombinant Escherichia coli by reducing tryptone concentration
by: Che Man, Rohaida, et al.
Published: (2023)

Effects of Culture Conditions of Immobilized Recombinant Escherichia coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Stability
by: Rohaida, Che Man, et al.
Published: (2016)

Effects of culture conditions of immobilized recombinant Escherichia coli on cyclodextrin glucanotransferase (CGTase) excretion and cell stability
by: Mohd Fuzi/, Siti Fatimah Zaharah, et al.
Published: (2016)

Effects of culture conditions of immobilized recombinant Escherichia coli on cyclodextrin glucanotransferase (CGTase) excretionand cell stability
by: Che Man, Rohaida, et al.
Published: (2016)

Excretory over-expression of Bacillus sp. G1 cyclodextrin glucanotransferase (CGTase) in Escherichia coli: Optimization of the cultivation conditions by response surface methodology
by: Lo, Po Kim, et al.
Published: (2007)

Effect of the Cross Linkers’ Concentration on the Immobilization of Recombinant Escherichia Coli Cells on Hollow Fiber Membrane for Excretion of Cyclodextrin Glucanotransferase (CGTase)
by: Rohaida, Che Man, et al.
Published: (2017)

Secretory overexpression of CGTase gene from bacillus sp. G1 in escherichia coli
by: Lo, Po Kim, et al.
Published: (2005)

Effects of buffer properties on cyclodextrin glucanotransferase reactions and cyclodextrin production from raw sago (cycas revoluta) starch
by: Kamaruddin, Kamarulzaman, et al.
Published: (2005)

Characterization of α-Cyclodextrin Glucanotransferase from Bacillus licheniformis
by: N., Jamil, et al.
Published: (2017)

Effect of reaction parameters on the production of cyclodextrin using cyclodextrin glucanotransferase from bacillus licheniformis
by: Rohaida, Che Man, et al.
Published: (2020)

Immobilization of cyclodextrin glucanotransferase on polyvinylidene fluoride hollow fiber membrane for cyclodextrin production
by: Natassha, Jamil
Published: (2019)

Optimization of medium for the production of β-cyclodextrin glucanotransferase using Central Composite Design (CCD)
by: Ibrahim, Hanan Moawia, et al.
Published: (2005)

Facilitative recovery of cyclodextrin glucanotransferase using chromatographic strategies
by: Sivapragasam, Magaret
Published: (2014)

Immobilization of cyclodextrin glucanotransferase on electrospun polyvinyl alcohol nanofibers
by: Saallah, Suryani
Published: (2014)

Stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane
by: S., Suhaimi, et al.
Published: (2018)

Production of cyclodextrin glucanotransferase (CGTase) from Bacillus sp. TS1-1: process optimisation
by: Ahmad Tarmizi, Azmil Haizam, et al.
Published: (2003)

Purification and characterization of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. G1
by: Ho, Kek Sian, et al.
Published: (2005)

Production of cyclodextrin glucanotransferase (CGTase) from Bacillus stearothermophilus HR1 in shake flasks
by: Wong, Tien Ee, et al.
Published: (2003)

Bacteriocin release protein mediated secretory expression of recombinant chalcone synthase in Escherichia coli
by: Zakaria, Iffah Izzati, et al.
Published: (2011)

Optimization of reaction conditions for the production of cyclodextrin (CD) using cyclodextrin glucanotransferase (CGTase) immobilized on hollow fiber membrane
by: S., Suhaimi, et al.
Published: (2020)

Partial purification of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. TS1-1
by: Mohd Azim, Abdul Rahman
Published: (2008)

Partial characterization of cyclodextrin glucanotransferase by Bacillus sp. TS1-1
by: Ummi Nor Husna, Husin
Published: (2009)

Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning
by: Saallah, Suryani, et al.
Published: (2016)

Preparation and characterisation of Cyclodextrin glucanotransferase enzyme immobilised in electrospun nanofibrous membrane
by: Saallah, Suryani, et al.
Published: (2017)

Optimization of Process Parameters of Immobilized Escherichia Coli for Cyclodextrin Production
by: Nur Nadia, Mohd Zakaria, et al.
Published: (2023)

Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane
by: Natassha, Jamil, et al.
Published: (2018)

Recovery of cyclodextrin glucanotransferase (CGTase) using immobilized metal chelating affinity chromatography
by: Sivapragasam, Magaret, et al.
Published: (2015)

Molecular cloning of Cyclodextrin glucanotransferase gene from Bacillus sp. G1
by: Ong, Rui Min
Published: (2005)

Molecular cloning of a cyclodextrin glucanotransferase gene from alkalophilic Bacillus sp. TS1-1 and characterization of the recombinant enzyme
by: Rahman, Kamalesh, et al.
Published: (2006)

Immobilization of cyclodextrin glucanotransferase on hollow fiber membrane: Optimization of the immobilization parameters by response surface methodology
by: Rohaida, Che Man, et al.
Published: (2019)

Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology
by: Abd. Rahman, Roshanida, et al.
Published: (2004)

Dynamic mathematical modelling of reaction kinetics for Cyclodextrins production from different starch sources using Bacillus macerans cyclodextrin glucanotransferase
by: Shahrazi, Syahinaz, et al.
Published: (2013)

Production of cyclodextrin glucanotransferase by Bacillus circulans P28 using sago starch as substrate
by: Mansor, Azlina
Published: (2013)

Production of cyclodextrin (cd) using immobilized recombinant Escherichia coli
by: Rohaida, Che Man, et al.
Published: (2021)

Production of cyclodextrin glucanotransferase (CGTase) from alkalophilic Bacillus sp. TS1-1: Media optimization using experimental design
by: Mahat, Mohd Khairizal, et al.
Published: (2004)

Transformation of cyclodextrin glucanotransferase (CGTase) from aqueous suspension to fine solid particles via electrospraying
by: Saallah, Suryani, et al.
Published: (2014)