Impact of dengue virus (serotype DENV-2) infection on liver of BALB/c mice: a histopathological analysis
In this research, we characterized the histopathological impact of dengue virus (serotype DENV-2) infection in livers of BALB/c mice. The mice were infected with different doses of DENV-2 via intraperitoneal injection and liver tissues were processed for histological analyses and variation was docum...
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| Format: | Article |
| Language: | English |
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Elsevier
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14411/ http://psasir.upm.edu.my/id/eprint/14411/1/Impact%20of%20dengue%20virus%20%28serotype%20DENV-2%29%20infection%20on%20liver%20of%20BALBc%20mice%20a%20histopathological%20analysis.pdf |
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| author | Syed, Sakinah Priya, Sivan Padma Kumaran, Sharmilah Kumari Fatin Amira Krishnan, Poorani Alsaeedy, Hiba Mok, Pooi Ling Chee, Hui Yee Higuchi, Akon Alarfaj, Abdullah A. Munusamy, Murugan A. Murugan, Kadarkarai Mat Taib, Che Norma Arulselvan, Palanisamy Rajan, Mariappan Neela, Vasantha Kumari Awang Hamat, Rukman Benelli, Giovanni Subbiah, Suresh Kumar |
| author_facet | Syed, Sakinah Priya, Sivan Padma Kumaran, Sharmilah Kumari Fatin Amira Krishnan, Poorani Alsaeedy, Hiba Mok, Pooi Ling Chee, Hui Yee Higuchi, Akon Alarfaj, Abdullah A. Munusamy, Murugan A. Murugan, Kadarkarai Mat Taib, Che Norma Arulselvan, Palanisamy Rajan, Mariappan Neela, Vasantha Kumari Awang Hamat, Rukman Benelli, Giovanni Subbiah, Suresh Kumar |
| author_sort | Syed, Sakinah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this research, we characterized the histopathological impact of dengue virus (serotype DENV-2) infection in livers of BALB/c mice. The mice were infected with different doses of DENV-2 via intraperitoneal injection and liver tissues were processed for histological analyses and variation was documented. In the BALB/c mouse model, typical liver tissues showed regular hepatocyte architecture, with normal endothelial cells surrounding sinusoid capillary. Based on histopathological observations, the liver sections of BALB/c mice infected by DENV-2 exhibited a loss of cell integrity, with a widening of the sinusoidal spaces. There were marked increases in the infiltration of mononuclear cells. The areas of hemorrhage and micro- and macrovesicular steatosis were noted. Necrosis and apoptosis were abundantly present. The hallmark of viral infection, i.e., cytopathic effects, included intracellular edema and vacuole formation, cumulatively led to sinusoidal and lobular collapse in the liver. The histopathological studies on autopsy specimens of fatal human DENV cases are important to shed light on tissue damage for preventive and treatment modalities, in order to manage future DENV infections. In this framework, the method present here on BALB/c mouse model may be used to study not only the effects of infections by other DENV serotypes, but also to investigate the effects of novel drugs, such as recently developed nano-formulations, and the relative recovery ability with intact immune functions of host. |
| first_indexed | 2025-11-15T07:58:18Z |
| format | Article |
| id | upm-14411 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:58:18Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-144112019-04-08T08:37:25Z http://psasir.upm.edu.my/id/eprint/14411/ Impact of dengue virus (serotype DENV-2) infection on liver of BALB/c mice: a histopathological analysis Syed, Sakinah Priya, Sivan Padma Kumaran, Sharmilah Kumari Fatin Amira Krishnan, Poorani Alsaeedy, Hiba Mok, Pooi Ling Chee, Hui Yee Higuchi, Akon Alarfaj, Abdullah A. Munusamy, Murugan A. Murugan, Kadarkarai Mat Taib, Che Norma Arulselvan, Palanisamy Rajan, Mariappan Neela, Vasantha Kumari Awang Hamat, Rukman Benelli, Giovanni Subbiah, Suresh Kumar In this research, we characterized the histopathological impact of dengue virus (serotype DENV-2) infection in livers of BALB/c mice. The mice were infected with different doses of DENV-2 via intraperitoneal injection and liver tissues were processed for histological analyses and variation was documented. In the BALB/c mouse model, typical liver tissues showed regular hepatocyte architecture, with normal endothelial cells surrounding sinusoid capillary. Based on histopathological observations, the liver sections of BALB/c mice infected by DENV-2 exhibited a loss of cell integrity, with a widening of the sinusoidal spaces. There were marked increases in the infiltration of mononuclear cells. The areas of hemorrhage and micro- and macrovesicular steatosis were noted. Necrosis and apoptosis were abundantly present. The hallmark of viral infection, i.e., cytopathic effects, included intracellular edema and vacuole formation, cumulatively led to sinusoidal and lobular collapse in the liver. The histopathological studies on autopsy specimens of fatal human DENV cases are important to shed light on tissue damage for preventive and treatment modalities, in order to manage future DENV infections. In this framework, the method present here on BALB/c mouse model may be used to study not only the effects of infections by other DENV serotypes, but also to investigate the effects of novel drugs, such as recently developed nano-formulations, and the relative recovery ability with intact immune functions of host. Elsevier 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/14411/1/Impact%20of%20dengue%20virus%20%28serotype%20DENV-2%29%20infection%20on%20liver%20of%20BALBc%20mice%20a%20histopathological%20analysis.pdf Syed, Sakinah and Priya, Sivan Padma and Kumaran, Sharmilah Kumari and Fatin Amira and Krishnan, Poorani and Alsaeedy, Hiba and Mok, Pooi Ling and Chee, Hui Yee and Higuchi, Akon and Alarfaj, Abdullah A. and Munusamy, Murugan A. and Murugan, Kadarkarai and Mat Taib, Che Norma and Arulselvan, Palanisamy and Rajan, Mariappan and Neela, Vasantha Kumari and Awang Hamat, Rukman and Benelli, Giovanni and Subbiah, Suresh Kumar (2017) Impact of dengue virus (serotype DENV-2) infection on liver of BALB/c mice: a histopathological analysis. Tissue and Cell, 49 (1). pp. 86-94. ISSN 0040-8166; ESSN: 1532-3072 https://www.sciencedirect.com/science/article/pii/S0040816616301926#! 10.1016/j.tice.2016.11.005 |
| spellingShingle | Syed, Sakinah Priya, Sivan Padma Kumaran, Sharmilah Kumari Fatin Amira Krishnan, Poorani Alsaeedy, Hiba Mok, Pooi Ling Chee, Hui Yee Higuchi, Akon Alarfaj, Abdullah A. Munusamy, Murugan A. Murugan, Kadarkarai Mat Taib, Che Norma Arulselvan, Palanisamy Rajan, Mariappan Neela, Vasantha Kumari Awang Hamat, Rukman Benelli, Giovanni Subbiah, Suresh Kumar Impact of dengue virus (serotype DENV-2) infection on liver of BALB/c mice: a histopathological analysis |
| title | Impact of dengue virus (serotype DENV-2) infection on liver of BALB/c mice: a histopathological analysis |
| title_full | Impact of dengue virus (serotype DENV-2) infection on liver of BALB/c mice: a histopathological analysis |
| title_fullStr | Impact of dengue virus (serotype DENV-2) infection on liver of BALB/c mice: a histopathological analysis |
| title_full_unstemmed | Impact of dengue virus (serotype DENV-2) infection on liver of BALB/c mice: a histopathological analysis |
| title_short | Impact of dengue virus (serotype DENV-2) infection on liver of BALB/c mice: a histopathological analysis |
| title_sort | impact of dengue virus (serotype denv-2) infection on liver of balb/c mice: a histopathological analysis |
| url | http://psasir.upm.edu.my/id/eprint/14411/ http://psasir.upm.edu.my/id/eprint/14411/ http://psasir.upm.edu.my/id/eprint/14411/ http://psasir.upm.edu.my/id/eprint/14411/1/Impact%20of%20dengue%20virus%20%28serotype%20DENV-2%29%20infection%20on%20liver%20of%20BALBc%20mice%20a%20histopathological%20analysis.pdf |