Effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (Ob-db) rats
In this present study, we investigated the effects of cocoa extract containing polyphenols and methylxanthines prepared from cocoa powder on the biochemical parameters of obese-diabetic (Obdb) rats. Obese-diabetic (Ob-db) rats were developed using a high-fat diet (49% fat, 32% carbohydrate, and 19%...
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| Language: | English English |
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American Chemical Society
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14351/ http://psasir.upm.edu.my/id/eprint/14351/1/Effects%20of%20cocoa%20extract%20on%20glucometabolism.pdf |
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| author | Mhd Jalil, Abbe Maleyki Ismail, Amin Chong, Pei Pei Hamid, Muhajir Syed Kamaruddin, Syed Hasbullah |
| author_facet | Mhd Jalil, Abbe Maleyki Ismail, Amin Chong, Pei Pei Hamid, Muhajir Syed Kamaruddin, Syed Hasbullah |
| author_sort | Mhd Jalil, Abbe Maleyki |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this present study, we investigated the effects of cocoa extract containing polyphenols and methylxanthines prepared from cocoa powder on the biochemical parameters of obese-diabetic (Obdb) rats. Obese-diabetic (Ob-db) rats were developed using a high-fat diet (49% fat, 32% carbohydrate,
and 19% protein from total energy, kcal) for 3 months, followed by a low dose (35 mg/kg body weight)streptozotocin (STZ) injection. Cocoa extract (600 mg/kg body weight/day) was given to the rats for 4 weeks. The results indicated that there were no significant differences in fasting plasma glucose and insulin level after 4 weeks of cocoa extract administration. Oral glucose tolerance test revealed
that cocoa supplementation in Ob-db rats significantly (p < 0.05) reduced plasma glucose at 60 and 90 min compared to unsupplemented Ob-db rats. Plasma free fatty acid and oxidative stress biomarker(8-isoprostane) were significantly (p < 0.05) reduced after cocoa supplementation. Superoxide dismutase activity was enhanced in Ob-db compared to that in nonsupplemented rats. However, no change was observed in catalase activity. The results showed that cocoa supplementation had an effect on postprandial glucose control but not for long term (4 weeks). Moreover, cocoa supplementation could reduce circulating plasma free fatty acid and 8-isoprostane and may enhance the antioxidant defense system. |
| first_indexed | 2025-11-15T07:58:02Z |
| format | Article |
| id | upm-14351 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:58:02Z |
| publishDate | 2008 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-143512015-10-23T08:09:55Z http://psasir.upm.edu.my/id/eprint/14351/ Effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (Ob-db) rats Mhd Jalil, Abbe Maleyki Ismail, Amin Chong, Pei Pei Hamid, Muhajir Syed Kamaruddin, Syed Hasbullah In this present study, we investigated the effects of cocoa extract containing polyphenols and methylxanthines prepared from cocoa powder on the biochemical parameters of obese-diabetic (Obdb) rats. Obese-diabetic (Ob-db) rats were developed using a high-fat diet (49% fat, 32% carbohydrate, and 19% protein from total energy, kcal) for 3 months, followed by a low dose (35 mg/kg body weight)streptozotocin (STZ) injection. Cocoa extract (600 mg/kg body weight/day) was given to the rats for 4 weeks. The results indicated that there were no significant differences in fasting plasma glucose and insulin level after 4 weeks of cocoa extract administration. Oral glucose tolerance test revealed that cocoa supplementation in Ob-db rats significantly (p < 0.05) reduced plasma glucose at 60 and 90 min compared to unsupplemented Ob-db rats. Plasma free fatty acid and oxidative stress biomarker(8-isoprostane) were significantly (p < 0.05) reduced after cocoa supplementation. Superoxide dismutase activity was enhanced in Ob-db compared to that in nonsupplemented rats. However, no change was observed in catalase activity. The results showed that cocoa supplementation had an effect on postprandial glucose control but not for long term (4 weeks). Moreover, cocoa supplementation could reduce circulating plasma free fatty acid and 8-isoprostane and may enhance the antioxidant defense system. American Chemical Society 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14351/1/Effects%20of%20cocoa%20extract%20on%20glucometabolism.pdf Mhd Jalil, Abbe Maleyki and Ismail, Amin and Chong, Pei Pei and Hamid, Muhajir and Syed Kamaruddin, Syed Hasbullah (2008) Effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (Ob-db) rats. Journal of Agricultural and Food Chemistry, 56 (17). pp. 7877-7884. ISSN 0021-8561 Cocoa Theobromine Caffeine English |
| spellingShingle | Cocoa Theobromine Caffeine Mhd Jalil, Abbe Maleyki Ismail, Amin Chong, Pei Pei Hamid, Muhajir Syed Kamaruddin, Syed Hasbullah Effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (Ob-db) rats |
| title | Effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (Ob-db) rats |
| title_full | Effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (Ob-db) rats |
| title_fullStr | Effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (Ob-db) rats |
| title_full_unstemmed | Effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (Ob-db) rats |
| title_short | Effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (Ob-db) rats |
| title_sort | effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (ob-db) rats |
| topic | Cocoa Theobromine Caffeine |
| url | http://psasir.upm.edu.my/id/eprint/14351/ http://psasir.upm.edu.my/id/eprint/14351/1/Effects%20of%20cocoa%20extract%20on%20glucometabolism.pdf |