Effect of Pluronic F-68, 5% CO2 atmosphere, HEPES, and antibiotic-antimycotic on suspension adapted 293 cells
The influence of different parameters upon cell culture of serum-free adapted 293 cells including the surfactant Pluronic F-68, carbon dioxide (5% CO2 atmosphere), buffer HEPES and antibiotic-antimycotic has been explored. A defined serum-free medium (SFM) formulated without human or animal origin c...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Bentham Open
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14292/ http://psasir.upm.edu.my/id/eprint/14292/1/Effect%20of%20Pluronic%20F-68%2C%205%25%20CO2%20atmosphere%2C%20HEPES%2C%20and%20antibiotic-antimycotic%20on%20suspension%20adapted%20293%20cells.pdf |
| _version_ | 1848842352865574912 |
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| author | Negrete, Alejandro Ling, Tau Chuan Lyddiatt, Andrew |
| author_facet | Negrete, Alejandro Ling, Tau Chuan Lyddiatt, Andrew |
| author_sort | Negrete, Alejandro |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The influence of different parameters upon cell culture of serum-free adapted 293 cells including the surfactant Pluronic F-68, carbon dioxide (5% CO2 atmosphere), buffer HEPES and antibiotic-antimycotic has been explored. A defined serum-free medium (SFM) formulated without human or animal origin components from Invitrogen was used to grow the suspension adapted 293 cells. For all cell culture parameters cell density and viability of the suspension adapted 293 cells were monitored. The results indicated that the PF68 concentrations ranging from 0.05% to 0.2% can be used in the culture of the suspension adapted 293 cells since no negative effect upon either cell density or viability was detected. This will minimize the formation of aggregates during cell culture. It was demonstrated that neither the cell density nor the viability of the suspension adapted 293 cells were affected by the 5% CO2 atmosphere at the inoculation cell densities evaluated. The use of the buffer HEPES in the cultivation of suspension adapted 293 cells did not cause negative effects upon cell density and viability. The addition of HEPES makes more robust the culture to pH fluctuations. The antibiotic-antimycotic can be used when needed at concentrations of up to 50 IU/ml for the culture of this particular cell line, with no apparent effect upon cell growth. The results obtained will contribute to a basic understanding of the 293 cell culture in the 293 SFM II and to the process development of their culture in bioreactors for the expression of different products of biotechnology interest. |
| first_indexed | 2025-11-15T07:57:46Z |
| format | Article |
| id | upm-14292 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:57:46Z |
| publishDate | 2008 |
| publisher | Bentham Open |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-142922018-10-08T02:51:49Z http://psasir.upm.edu.my/id/eprint/14292/ Effect of Pluronic F-68, 5% CO2 atmosphere, HEPES, and antibiotic-antimycotic on suspension adapted 293 cells Negrete, Alejandro Ling, Tau Chuan Lyddiatt, Andrew The influence of different parameters upon cell culture of serum-free adapted 293 cells including the surfactant Pluronic F-68, carbon dioxide (5% CO2 atmosphere), buffer HEPES and antibiotic-antimycotic has been explored. A defined serum-free medium (SFM) formulated without human or animal origin components from Invitrogen was used to grow the suspension adapted 293 cells. For all cell culture parameters cell density and viability of the suspension adapted 293 cells were monitored. The results indicated that the PF68 concentrations ranging from 0.05% to 0.2% can be used in the culture of the suspension adapted 293 cells since no negative effect upon either cell density or viability was detected. This will minimize the formation of aggregates during cell culture. It was demonstrated that neither the cell density nor the viability of the suspension adapted 293 cells were affected by the 5% CO2 atmosphere at the inoculation cell densities evaluated. The use of the buffer HEPES in the cultivation of suspension adapted 293 cells did not cause negative effects upon cell density and viability. The addition of HEPES makes more robust the culture to pH fluctuations. The antibiotic-antimycotic can be used when needed at concentrations of up to 50 IU/ml for the culture of this particular cell line, with no apparent effect upon cell growth. The results obtained will contribute to a basic understanding of the 293 cell culture in the 293 SFM II and to the process development of their culture in bioreactors for the expression of different products of biotechnology interest. Bentham Open 2008 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/14292/1/Effect%20of%20Pluronic%20F-68%2C%205%25%20CO2%20atmosphere%2C%20HEPES%2C%20and%20antibiotic-antimycotic%20on%20suspension%20adapted%20293%20cells.pdf Negrete, Alejandro and Ling, Tau Chuan and Lyddiatt, Andrew (2008) Effect of Pluronic F-68, 5% CO2 atmosphere, HEPES, and antibiotic-antimycotic on suspension adapted 293 cells. The Open Biotechnology Journal, 2. pp. 229-234. ISSN 1874-0707 https://benthamopen.com/ABSTRACT/TOBIOTJ-2-229 10.2174/1874070700802010229 |
| spellingShingle | Negrete, Alejandro Ling, Tau Chuan Lyddiatt, Andrew Effect of Pluronic F-68, 5% CO2 atmosphere, HEPES, and antibiotic-antimycotic on suspension adapted 293 cells |
| title | Effect of Pluronic F-68, 5% CO2 atmosphere, HEPES, and antibiotic-antimycotic on suspension adapted 293 cells |
| title_full | Effect of Pluronic F-68, 5% CO2 atmosphere, HEPES, and antibiotic-antimycotic on suspension adapted 293 cells |
| title_fullStr | Effect of Pluronic F-68, 5% CO2 atmosphere, HEPES, and antibiotic-antimycotic on suspension adapted 293 cells |
| title_full_unstemmed | Effect of Pluronic F-68, 5% CO2 atmosphere, HEPES, and antibiotic-antimycotic on suspension adapted 293 cells |
| title_short | Effect of Pluronic F-68, 5% CO2 atmosphere, HEPES, and antibiotic-antimycotic on suspension adapted 293 cells |
| title_sort | effect of pluronic f-68, 5% co2 atmosphere, hepes, and antibiotic-antimycotic on suspension adapted 293 cells |
| url | http://psasir.upm.edu.my/id/eprint/14292/ http://psasir.upm.edu.my/id/eprint/14292/ http://psasir.upm.edu.my/id/eprint/14292/ http://psasir.upm.edu.my/id/eprint/14292/1/Effect%20of%20Pluronic%20F-68%2C%205%25%20CO2%20atmosphere%2C%20HEPES%2C%20and%20antibiotic-antimycotic%20on%20suspension%20adapted%20293%20cells.pdf |