Effect of boiling and stir frying on total phenolics, carotenoids and radical scavenging activity of pumpkin (Cucurbita moschato)

Effect of various cooking methods on antioxidant content and radical scavenging activity of pumpkin was evaluated. Pumpkin (Cucurbita moschata) was boiled and stir-fried for 2, 4 and 6 minutes respectively. Beta-carotene and lycopene were determined using HPLC and total phenolics measured using Foli...

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Main Authors: Abdul Hamid, Azizah, Kerk, Chai Wee, Osman, Azizah, Misran, Azizah
Format: Article
Language:English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2009
Online Access:http://psasir.upm.edu.my/id/eprint/14207/
http://psasir.upm.edu.my/id/eprint/14207/1/14207.pdf
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author Abdul Hamid, Azizah
Kerk, Chai Wee
Osman, Azizah
Misran, Azizah
author_facet Abdul Hamid, Azizah
Kerk, Chai Wee
Osman, Azizah
Misran, Azizah
author_sort Abdul Hamid, Azizah
building UPM Institutional Repository
collection Online Access
description Effect of various cooking methods on antioxidant content and radical scavenging activity of pumpkin was evaluated. Pumpkin (Cucurbita moschata) was boiled and stir-fried for 2, 4 and 6 minutes respectively. Beta-carotene and lycopene were determined using HPLC and total phenolics measured using Folin-Ciocalteu method. The free radical scavenging activity of the samples was determined using 1, 1-diphenyl-2-picrylhydrazyl assay. Interestingly, result of the study showed an increase in both beta-carotene (2 to 4 times) and lycopene (17 to 40 times) content of pumpkin after cooking for 2, 4 and 6 minutes. However, the treatment resulted in 18 to 54% losses of total phenolics content of the pumpkin. Nevertheless, the free radical scavenging activity exhibited by cooked pumpkins was found to be high, in the range of 81.1% to 94.6% with IC50 of 1.41 to 1.62mg ml-1.
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spelling upm-142072015-09-25T02:09:36Z http://psasir.upm.edu.my/id/eprint/14207/ Effect of boiling and stir frying on total phenolics, carotenoids and radical scavenging activity of pumpkin (Cucurbita moschato) Abdul Hamid, Azizah Kerk, Chai Wee Osman, Azizah Misran, Azizah Effect of various cooking methods on antioxidant content and radical scavenging activity of pumpkin was evaluated. Pumpkin (Cucurbita moschata) was boiled and stir-fried for 2, 4 and 6 minutes respectively. Beta-carotene and lycopene were determined using HPLC and total phenolics measured using Folin-Ciocalteu method. The free radical scavenging activity of the samples was determined using 1, 1-diphenyl-2-picrylhydrazyl assay. Interestingly, result of the study showed an increase in both beta-carotene (2 to 4 times) and lycopene (17 to 40 times) content of pumpkin after cooking for 2, 4 and 6 minutes. However, the treatment resulted in 18 to 54% losses of total phenolics content of the pumpkin. Nevertheless, the free radical scavenging activity exhibited by cooked pumpkins was found to be high, in the range of 81.1% to 94.6% with IC50 of 1.41 to 1.62mg ml-1. Faculty of Food Science and Technology, Universiti Putra Malaysia 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14207/1/14207.pdf Abdul Hamid, Azizah and Kerk, Chai Wee and Osman, Azizah and Misran, Azizah (2009) Effect of boiling and stir frying on total phenolics, carotenoids and radical scavenging activity of pumpkin (Cucurbita moschato). International Food Research Journal, 16 (1). pp. 45-51. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/16%20%281%29%202009/06%20IFRJ-2008-123%20Azizah%20Malaysia%20FINAL.pdf
spellingShingle Abdul Hamid, Azizah
Kerk, Chai Wee
Osman, Azizah
Misran, Azizah
Effect of boiling and stir frying on total phenolics, carotenoids and radical scavenging activity of pumpkin (Cucurbita moschato)
title Effect of boiling and stir frying on total phenolics, carotenoids and radical scavenging activity of pumpkin (Cucurbita moschato)
title_full Effect of boiling and stir frying on total phenolics, carotenoids and radical scavenging activity of pumpkin (Cucurbita moschato)
title_fullStr Effect of boiling and stir frying on total phenolics, carotenoids and radical scavenging activity of pumpkin (Cucurbita moschato)
title_full_unstemmed Effect of boiling and stir frying on total phenolics, carotenoids and radical scavenging activity of pumpkin (Cucurbita moschato)
title_short Effect of boiling and stir frying on total phenolics, carotenoids and radical scavenging activity of pumpkin (Cucurbita moschato)
title_sort effect of boiling and stir frying on total phenolics, carotenoids and radical scavenging activity of pumpkin (cucurbita moschato)
url http://psasir.upm.edu.my/id/eprint/14207/
http://psasir.upm.edu.my/id/eprint/14207/
http://psasir.upm.edu.my/id/eprint/14207/1/14207.pdf