Effect of agitation and aeration rates on chitinase production using Trichoderma virens UKM1 in 2 L stirred tank reactor

Shrimps have been a popular raw material for the burgeoning marine and food industry contributing to increasing marine waste. Shrimp waste, which is rich in organic compounds is an abundant source of chitin, a natural polymer of N-acetyl-D-glucosamine (GluNac), a reducing sugar. For this respect, ch...

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Main Authors: Abd. Aziz, Suraini, Hassan, Mohd Ali, Fernandez, Christine C., Md Salleh, Madihah, Md Illias, Rosli
Format: Article
Language:English
English
Published: Humana Press 2008
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/14203/
http://psasir.upm.edu.my/id/eprint/14203/1/Effect%20of%20agitation%20and%20aeration%20rates%20on%20chitinase%20production%20using%20Trichoderma%20virens%20UKM1%20in%202%20L%20stirred%20tank%20reactor.pdf
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author Abd. Aziz, Suraini
Hassan, Mohd Ali
Fernandez, Christine C.
Md Salleh, Madihah
Md Illias, Rosli
author_facet Abd. Aziz, Suraini
Hassan, Mohd Ali
Fernandez, Christine C.
Md Salleh, Madihah
Md Illias, Rosli
author_sort Abd. Aziz, Suraini
building UPM Institutional Repository
collection Online Access
description Shrimps have been a popular raw material for the burgeoning marine and food industry contributing to increasing marine waste. Shrimp waste, which is rich in organic compounds is an abundant source of chitin, a natural polymer of N-acetyl-D-glucosamine (GluNac), a reducing sugar. For this respect, chitinase-producing fungi have been extensively studied as biocontrol agents. Locally isolated Trichoderma virens UKM1 was used in this study. The effect of agitation and aeration rates using colloidal chitin as control substrate in a 2-l stirred tank reactor gave the best agitation and aeration rates at 200 rpm and 0.33 vvm with 4.1 U/l per hour and 5.97 U/l per hour of maximum volumetric chitinase activity obtained, respectively. Microscopic observations showed shear sensitivity at higher agitation rate of the above system. The oxygen uptake rate during the highest chitinase productivity obtained using sun-dried ground shrimp waste of 1.74 mg of dissolved oxygen per gram of fungal biomass per hour at the kappaL a of 8.34 per hour.
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spelling upm-142032015-12-03T03:54:57Z http://psasir.upm.edu.my/id/eprint/14203/ Effect of agitation and aeration rates on chitinase production using Trichoderma virens UKM1 in 2 L stirred tank reactor Abd. Aziz, Suraini Hassan, Mohd Ali Fernandez, Christine C. Md Salleh, Madihah Md Illias, Rosli Shrimps have been a popular raw material for the burgeoning marine and food industry contributing to increasing marine waste. Shrimp waste, which is rich in organic compounds is an abundant source of chitin, a natural polymer of N-acetyl-D-glucosamine (GluNac), a reducing sugar. For this respect, chitinase-producing fungi have been extensively studied as biocontrol agents. Locally isolated Trichoderma virens UKM1 was used in this study. The effect of agitation and aeration rates using colloidal chitin as control substrate in a 2-l stirred tank reactor gave the best agitation and aeration rates at 200 rpm and 0.33 vvm with 4.1 U/l per hour and 5.97 U/l per hour of maximum volumetric chitinase activity obtained, respectively. Microscopic observations showed shear sensitivity at higher agitation rate of the above system. The oxygen uptake rate during the highest chitinase productivity obtained using sun-dried ground shrimp waste of 1.74 mg of dissolved oxygen per gram of fungal biomass per hour at the kappaL a of 8.34 per hour. Humana Press 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14203/1/Effect%20of%20agitation%20and%20aeration%20rates%20on%20chitinase%20production%20using%20Trichoderma%20virens%20UKM1%20in%202%20L%20stirred%20tank%20reactor.pdf Abd. Aziz, Suraini and Hassan, Mohd Ali and Fernandez, Christine C. and Md Salleh, Madihah and Md Illias, Rosli (2008) Effect of agitation and aeration rates on chitinase production using Trichoderma virens UKM1 in 2 L stirred tank reactor. Applied Biochemistry and Biotechnology, 150 (2). pp. 193-204. ISSN 0273-2289 Chitinase Aeration tanks - Testing Reactors English
spellingShingle Chitinase
Aeration tanks - Testing
Reactors
Abd. Aziz, Suraini
Hassan, Mohd Ali
Fernandez, Christine C.
Md Salleh, Madihah
Md Illias, Rosli
Effect of agitation and aeration rates on chitinase production using Trichoderma virens UKM1 in 2 L stirred tank reactor
title Effect of agitation and aeration rates on chitinase production using Trichoderma virens UKM1 in 2 L stirred tank reactor
title_full Effect of agitation and aeration rates on chitinase production using Trichoderma virens UKM1 in 2 L stirred tank reactor
title_fullStr Effect of agitation and aeration rates on chitinase production using Trichoderma virens UKM1 in 2 L stirred tank reactor
title_full_unstemmed Effect of agitation and aeration rates on chitinase production using Trichoderma virens UKM1 in 2 L stirred tank reactor
title_short Effect of agitation and aeration rates on chitinase production using Trichoderma virens UKM1 in 2 L stirred tank reactor
title_sort effect of agitation and aeration rates on chitinase production using trichoderma virens ukm1 in 2 l stirred tank reactor
topic Chitinase
Aeration tanks - Testing
Reactors
url http://psasir.upm.edu.my/id/eprint/14203/
http://psasir.upm.edu.my/id/eprint/14203/1/Effect%20of%20agitation%20and%20aeration%20rates%20on%20chitinase%20production%20using%20Trichoderma%20virens%20UKM1%20in%202%20L%20stirred%20tank%20reactor.pdf