Distribution, morphological characterization and pathogenicity of Fusarium sacchari associated with pokkah boeng disease of sugarcane in Peninsular Malaysia
Pokkah boeng disease on sugarcane has been recorded in almost all countries where sugarcane is growl)commercially. The objectives of this study were to survey the distribution of Fusarium sacchari associated with pokkah boeng disease throughout Peninsular Malaysia, to isolate and identify the causal...
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14115/ http://psasir.upm.edu.my/id/eprint/14115/1/Distribution%2C%20morphological%20characterization%20and%20pathogenicity%20of%20Fusarium%20sacchari%20associated%20with%20pokkah%20boeng%20disease%20of%20sugarcane%20in%20Peninsular%20Malaysia.pdf |
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| author | Mohamed Sidique, Siti Nordahliawate Mohd Zainuddin, Nur Ain Izzati Abdul Razak, Azmi Salleh, Baharuddin |
| author_facet | Mohamed Sidique, Siti Nordahliawate Mohd Zainuddin, Nur Ain Izzati Abdul Razak, Azmi Salleh, Baharuddin |
| author_sort | Mohamed Sidique, Siti Nordahliawate |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Pokkah boeng disease on sugarcane has been recorded in almost all countries where sugarcane is growl)commercially. The objectives of this study were to survey the distribution of Fusarium sacchari associated with pokkah boeng disease throughout Peninsular Malaysia, to isolate and identify the causal organisms by using morphological characteristics, and to ascertain the pathogenicity of F. sacchari based on Koch's postulates. A total of 58 strains of F. sacchari were obtained throughout sugarcane plantations, small holders and household compounds within seven states i.e. Kedah, Perlis, Penang, Kelantan, Terengganu, Pahang and Johor in Peninsular Malaysia. The highest number of F: sacchari strains was obtained from Kedah (48%), followed by Perlis (25%), Penang (3%), Pahang (3%), Kelantan (5%), Terengganu (8%) and Johor (8%). For identification of F. sacchari, carnation leaves agar (CLA) and potato dextrose agar (PDA) media were used with emphasis for characterizations of colony features, growth rates, shapes and sizes of macroconidia and microconidia, conidiogeneous cells and chlamydospores. In plant house pathogenicity tests, healthy seedlings of sugarcane cultivar PS-81-362 were inoculated by injection and soaking techniques with conidial suspension (2x106 conidia/ml) of selected strains of F. sacchari, F. proliferatum and F. subglutinans. All strains of F. sacchari tested were pathogenic to sugarcane plants with disease severity index (DSI) varying from 0.3 to 5.0 (0 for no visible symptoms and 5 for plants with symptoms of twisted, wrinkled and shortened leaves or death). There were no significant (p>0.05) difference in DSI caused by strains of F. sacchari on variety PS-81-362 for both inoculation techniques, although they were significantly different compared with the control. This knowledge would be invaluable in developing our understanding on the interaction between F. sacchari with the host plants.
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| first_indexed | 2025-11-15T07:57:00Z |
| format | Article |
| id | upm-14115 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:57:00Z |
| publishDate | 2008 |
| publisher | Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-141152015-09-18T07:31:31Z http://psasir.upm.edu.my/id/eprint/14115/ Distribution, morphological characterization and pathogenicity of Fusarium sacchari associated with pokkah boeng disease of sugarcane in Peninsular Malaysia Mohamed Sidique, Siti Nordahliawate Mohd Zainuddin, Nur Ain Izzati Abdul Razak, Azmi Salleh, Baharuddin Pokkah boeng disease on sugarcane has been recorded in almost all countries where sugarcane is growl)commercially. The objectives of this study were to survey the distribution of Fusarium sacchari associated with pokkah boeng disease throughout Peninsular Malaysia, to isolate and identify the causal organisms by using morphological characteristics, and to ascertain the pathogenicity of F. sacchari based on Koch's postulates. A total of 58 strains of F. sacchari were obtained throughout sugarcane plantations, small holders and household compounds within seven states i.e. Kedah, Perlis, Penang, Kelantan, Terengganu, Pahang and Johor in Peninsular Malaysia. The highest number of F: sacchari strains was obtained from Kedah (48%), followed by Perlis (25%), Penang (3%), Pahang (3%), Kelantan (5%), Terengganu (8%) and Johor (8%). For identification of F. sacchari, carnation leaves agar (CLA) and potato dextrose agar (PDA) media were used with emphasis for characterizations of colony features, growth rates, shapes and sizes of macroconidia and microconidia, conidiogeneous cells and chlamydospores. In plant house pathogenicity tests, healthy seedlings of sugarcane cultivar PS-81-362 were inoculated by injection and soaking techniques with conidial suspension (2x106 conidia/ml) of selected strains of F. sacchari, F. proliferatum and F. subglutinans. All strains of F. sacchari tested were pathogenic to sugarcane plants with disease severity index (DSI) varying from 0.3 to 5.0 (0 for no visible symptoms and 5 for plants with symptoms of twisted, wrinkled and shortened leaves or death). There were no significant (p>0.05) difference in DSI caused by strains of F. sacchari on variety PS-81-362 for both inoculation techniques, although they were significantly different compared with the control. This knowledge would be invaluable in developing our understanding on the interaction between F. sacchari with the host plants. Universiti Putra Malaysia 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14115/1/Distribution%2C%20morphological%20characterization%20and%20pathogenicity%20of%20Fusarium%20sacchari%20associated%20with%20pokkah%20boeng%20disease%20of%20sugarcane%20in%20Peninsular%20Malaysia.pdf Mohamed Sidique, Siti Nordahliawate and Mohd Zainuddin, Nur Ain Izzati and Abdul Razak, Azmi and Salleh, Baharuddin (2008) Distribution, morphological characterization and pathogenicity of Fusarium sacchari associated with pokkah boeng disease of sugarcane in Peninsular Malaysia. Pertanika Journal of Tropical Agricultural Science, 31 (2). pp. 279-286. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2031%20(2)%20Aug.%202008/19%20Page%20279-286.pdf |
| spellingShingle | Mohamed Sidique, Siti Nordahliawate Mohd Zainuddin, Nur Ain Izzati Abdul Razak, Azmi Salleh, Baharuddin Distribution, morphological characterization and pathogenicity of Fusarium sacchari associated with pokkah boeng disease of sugarcane in Peninsular Malaysia |
| title | Distribution, morphological characterization and pathogenicity of Fusarium sacchari associated with pokkah boeng disease of sugarcane in Peninsular Malaysia |
| title_full | Distribution, morphological characterization and pathogenicity of Fusarium sacchari associated with pokkah boeng disease of sugarcane in Peninsular Malaysia |
| title_fullStr | Distribution, morphological characterization and pathogenicity of Fusarium sacchari associated with pokkah boeng disease of sugarcane in Peninsular Malaysia |
| title_full_unstemmed | Distribution, morphological characterization and pathogenicity of Fusarium sacchari associated with pokkah boeng disease of sugarcane in Peninsular Malaysia |
| title_short | Distribution, morphological characterization and pathogenicity of Fusarium sacchari associated with pokkah boeng disease of sugarcane in Peninsular Malaysia |
| title_sort | distribution, morphological characterization and pathogenicity of fusarium sacchari associated with pokkah boeng disease of sugarcane in peninsular malaysia |
| url | http://psasir.upm.edu.my/id/eprint/14115/ http://psasir.upm.edu.my/id/eprint/14115/ http://psasir.upm.edu.my/id/eprint/14115/1/Distribution%2C%20morphological%20characterization%20and%20pathogenicity%20of%20Fusarium%20sacchari%20associated%20with%20pokkah%20boeng%20disease%20of%20sugarcane%20in%20Peninsular%20Malaysia.pdf |