Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems.
An aqueous two-phase purification process was employed for the recovery of Burkholderia Pseudomallei lipase from fermentation broth. The partition behavior of Burkholderia Pseudomallei lipase was investigated with various parameters such as phase composition, tie-line length (TLL), volume ratio (VR...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer
2009
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/14082/ |
| _version_ | 1848842294802776064 |
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| author | Ooi, Chien Wei Tey, Beng Ti Hii, Siew Ling Ariff, Arbakariya Wu, Ho Shing Lan, John Chi Wei Juang, RueyShin Mustapa Kamal, Siti Mazlina Chuan, Ling Tau |
| author_facet | Ooi, Chien Wei Tey, Beng Ti Hii, Siew Ling Ariff, Arbakariya Wu, Ho Shing Lan, John Chi Wei Juang, RueyShin Mustapa Kamal, Siti Mazlina Chuan, Ling Tau |
| author_sort | Ooi, Chien Wei |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | An aqueous two-phase purification process was employed for the recovery of Burkholderia Pseudomallei lipase from
fermentation broth. The partition behavior of Burkholderia Pseudomallei lipase was investigated with various parameters such as phase composition, tie-line length (TLL), volume ratio (VR), sample loading, system pH, and addition of neutral salts.Optimum conditions for the purification of lipase were obtained in polyethylene glycol (PEG) 6000-potassium phosphate system using TLL of 42.2% (w/w), with VR of 2.70, and 1% (w/w) NaCl addition at pH 7 for 20% (w/w) crude load. Based on this system, the purification factor of lipase was enhanced to 12.42 fold, with a high yield of 93%. Hence, the simplicity and effectiveness of aqueous two-phase systems (ATPS) in the purification of lipase were proven in this study. |
| first_indexed | 2025-11-15T07:56:51Z |
| format | Article |
| id | upm-14082 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:56:51Z |
| publishDate | 2009 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-140822013-11-28T02:23:35Z http://psasir.upm.edu.my/id/eprint/14082/ Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems. Ooi, Chien Wei Tey, Beng Ti Hii, Siew Ling Ariff, Arbakariya Wu, Ho Shing Lan, John Chi Wei Juang, RueyShin Mustapa Kamal, Siti Mazlina Chuan, Ling Tau An aqueous two-phase purification process was employed for the recovery of Burkholderia Pseudomallei lipase from fermentation broth. The partition behavior of Burkholderia Pseudomallei lipase was investigated with various parameters such as phase composition, tie-line length (TLL), volume ratio (VR), sample loading, system pH, and addition of neutral salts.Optimum conditions for the purification of lipase were obtained in polyethylene glycol (PEG) 6000-potassium phosphate system using TLL of 42.2% (w/w), with VR of 2.70, and 1% (w/w) NaCl addition at pH 7 for 20% (w/w) crude load. Based on this system, the purification factor of lipase was enhanced to 12.42 fold, with a high yield of 93%. Hence, the simplicity and effectiveness of aqueous two-phase systems (ATPS) in the purification of lipase were proven in this study. Springer 2009 Article PeerReviewed Ooi, Chien Wei and Tey, Beng Ti and Hii, Siew Ling and Ariff, Arbakariya and Wu, Ho Shing and Lan, John Chi Wei and Juang, RueyShin and Mustapa Kamal, Siti Mazlina and Chuan, Ling Tau (2009) Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems. Biotechnology and Bioprocess Engineering, 14 (6). pp. 811-818. ISSN 1226-8372 Lipase. Lipase - Isolation & purification. 10.1007/s12257-008-0306-y English |
| spellingShingle | Lipase. Lipase - Isolation & purification. Ooi, Chien Wei Tey, Beng Ti Hii, Siew Ling Ariff, Arbakariya Wu, Ho Shing Lan, John Chi Wei Juang, RueyShin Mustapa Kamal, Siti Mazlina Chuan, Ling Tau Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems. |
| title | Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems. |
| title_full | Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems. |
| title_fullStr | Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems. |
| title_full_unstemmed | Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems. |
| title_short | Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems. |
| title_sort | direct purification of burkholderia pseudomallei lipase from fermentation broth using aqueous two-phase systems. |
| topic | Lipase. Lipase - Isolation & purification. |
| url | http://psasir.upm.edu.my/id/eprint/14082/ http://psasir.upm.edu.my/id/eprint/14082/ |