Differential adherence capacities of clinical isolates of Streptococcus pneumoniae at different growth states to human respiratory epithelial cells (in vitro)
This study was conducted to evaluate the adherence and invasion capacity of four clinically important pneumococcal serotypes at early-, mid- and late-log phases at 1 and 3 h incubation times to A549 cells-a human respiratory epithelial cell. We observed that adherence capacity varied among the diffe...
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| Format: | Article |
| Language: | English |
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Academic Journals
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14068/ http://psasir.upm.edu.my/id/eprint/14068/1/14068.pdf |
| _version_ | 1848842290931433472 |
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| author | Mohd Desa, Mohd Nasir Parasakthi, Navaratnam Vadivelu, Jamunarani Sekaran, Shamala Devi |
| author_facet | Mohd Desa, Mohd Nasir Parasakthi, Navaratnam Vadivelu, Jamunarani Sekaran, Shamala Devi |
| author_sort | Mohd Desa, Mohd Nasir |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study was conducted to evaluate the adherence and invasion capacity of four clinically important pneumococcal serotypes at early-, mid- and late-log phases at 1 and 3 h incubation times to A549 cells-a human respiratory epithelial cell. We observed that adherence capacity varied among the different isolates at all growth phases (pi 0.05) but generally, an individual isolate had a comparable adherence capacity at all levels of the log phase (p>0.05). Bacterial adherence also increased after a longer exposure to the host cells but significant differences were mostly observed at only early- and late-log phases at both incubation times. No bacterial invasion was however observed at 1 h post incubation but that at 3 h, a few bacteria penetrated the host cells but the bacterial cfu numbers were statistically insufficient for analysis. More studies are required to further confirm these observations. Streptococcus pneumoniae, epithelial cell, adherence, invasion, exponential growth phase. |
| first_indexed | 2025-11-15T07:56:47Z |
| format | Article |
| id | upm-14068 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:56:47Z |
| publishDate | 2008 |
| publisher | Academic Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-140682017-10-27T04:47:28Z http://psasir.upm.edu.my/id/eprint/14068/ Differential adherence capacities of clinical isolates of Streptococcus pneumoniae at different growth states to human respiratory epithelial cells (in vitro) Mohd Desa, Mohd Nasir Parasakthi, Navaratnam Vadivelu, Jamunarani Sekaran, Shamala Devi This study was conducted to evaluate the adherence and invasion capacity of four clinically important pneumococcal serotypes at early-, mid- and late-log phases at 1 and 3 h incubation times to A549 cells-a human respiratory epithelial cell. We observed that adherence capacity varied among the different isolates at all growth phases (pi 0.05) but generally, an individual isolate had a comparable adherence capacity at all levels of the log phase (p>0.05). Bacterial adherence also increased after a longer exposure to the host cells but significant differences were mostly observed at only early- and late-log phases at both incubation times. No bacterial invasion was however observed at 1 h post incubation but that at 3 h, a few bacteria penetrated the host cells but the bacterial cfu numbers were statistically insufficient for analysis. More studies are required to further confirm these observations. Streptococcus pneumoniae, epithelial cell, adherence, invasion, exponential growth phase. Academic Journals 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14068/1/14068.pdf Mohd Desa, Mohd Nasir and Parasakthi, Navaratnam and Vadivelu, Jamunarani and Sekaran, Shamala Devi (2008) Differential adherence capacities of clinical isolates of Streptococcus pneumoniae at different growth states to human respiratory epithelial cells (in vitro). Asian Journal of Cell Biology, 3 (2). pp. 61-66. ISSN 1814-0068; ESSN: 2152-3517 http://www.scialert.net/abstract/?doi=ajcb.2008.61.66 10.3923/ajcb.2008.61.66 |
| spellingShingle | Mohd Desa, Mohd Nasir Parasakthi, Navaratnam Vadivelu, Jamunarani Sekaran, Shamala Devi Differential adherence capacities of clinical isolates of Streptococcus pneumoniae at different growth states to human respiratory epithelial cells (in vitro) |
| title | Differential adherence capacities of clinical isolates of Streptococcus pneumoniae at different growth states to human respiratory epithelial cells (in vitro) |
| title_full | Differential adherence capacities of clinical isolates of Streptococcus pneumoniae at different growth states to human respiratory epithelial cells (in vitro) |
| title_fullStr | Differential adherence capacities of clinical isolates of Streptococcus pneumoniae at different growth states to human respiratory epithelial cells (in vitro) |
| title_full_unstemmed | Differential adherence capacities of clinical isolates of Streptococcus pneumoniae at different growth states to human respiratory epithelial cells (in vitro) |
| title_short | Differential adherence capacities of clinical isolates of Streptococcus pneumoniae at different growth states to human respiratory epithelial cells (in vitro) |
| title_sort | differential adherence capacities of clinical isolates of streptococcus pneumoniae at different growth states to human respiratory epithelial cells (in vitro) |
| url | http://psasir.upm.edu.my/id/eprint/14068/ http://psasir.upm.edu.my/id/eprint/14068/ http://psasir.upm.edu.my/id/eprint/14068/ http://psasir.upm.edu.my/id/eprint/14068/1/14068.pdf |