Development of a TaqMan real-time PCR for sensitive detection of the novel phytoplasma associated with coconut yellow decline in Malaysia.

QR Code

Development of a TaqMan real-time PCR for sensitive detection of the novel phytoplasma associated with coconut yellow decline in Malaysia.

Coconut yellow decline (CYD) disease in Malaysia has been shown to be associated with two different phytoplasmas, one from the 16SrXIV group and one from a novel taxonomic group. Here we report the development of a real-time PCR assay using a 16S rDNA-based TaqMan primer–probe set, for sensitive, qu...

Full description

Bibliographic Details
Main Authors:	Nejat, Naghmeh, Sijam, Kamaruzaman, Abdullah, Siti Nor Akmar, Vadamalai, Ganesan, Sidek, Zakaria, Dickinson, Matthew
Format:	Article
Language:	English English
Published:	EDIZIONI ETS 2010
Online Access:	http://psasir.upm.edu.my/id/eprint/14002/ http://psasir.upm.edu.my/id/eprint/14002/1/Development%20of%20a%20TaqMan%20real.pdf

Similar Items

Molecular characterization of a phytoplasma associated with coconut yellow decline (CYD) in Malaysia
by: Nejat, Naghmeh, et al.
Published: (2009)

First report of a 16SrXIV, 'Candidatus Phytoplasma cynodontis' group phytoplasma associated with coconut yellow decline in Malaysia
by: Nejat, Naghmeh, et al.
Published: (2009)

Detection and Molecular Characterization of Phytoplasma Associated with Coconut Yellow Decline
by: Nejat, Naghmeh
Published: (2009)

Molecular characterization of an aster yellows phytoplasma associated with proliferation of periwinkle in Malaysia
by: Nejat, Naghmeh, et al.
Published: (2010)

Phytoplasmas associated with disease of coconut in Malaysia: Phylogenetic groups and host plant species
by: Nejat, Naghmeh, et al.
Published: (2009)

First report of two distinct phytoplasma species, ‘Candidatus Phytoplasma cynodontis’ and ‘Candidatus Phytoplasma asteris,’ simultaneously associated with yellow decline of Wodyetia bifurcata (foxtail palm) in Malaysia
by: Naderali, N., et al.
Published: (2013)

Transcriptomics-based analysis using RNA-Seq of the coconut (Cocos nucifera) leaf in response to yellow decline phytoplasma infection
by: Nejat, Naghmeh, et al.
Published: (2015)

Phytoplasma detection in coconut palm and other tropical crops
by: Nejat, Naghmeh, et al.
Published: (2010)

Detection and identification of aster yellows phytoplasma associated with lipstick yellow frond disease in Malaysia
by: Naderali, Neda, et al.
Published: (2014)

A TaqMan real-time PCR assay for the detection and quantitation of Plasmodium knowlesi
by: Paul Cliff, Simon Divis, et al.
Published: (2010)

Meatball model of porcine DNA detection by TaqMan probe real-time PCR
by: Sajali, N., et al.
Published: (2022)

First report of Spiroplasma citri (-Induced) associated with periwinkle lethal yellows in Southeast Asia.
by: Nejat, Naghmeh, et al.
Published: (2011)

TaqMan real-time PCR for detection of pathogenic Leptospira spp. in canine clinical samples
by: Abdul Rahman, Mohammad Sabri, et al.
Published: (2023)

A TaqMan real-time PCR assay for Rhizoctonia cerealis and its use in wheat and soil
by: Woodhall, J.W., et al.
Published: (2017)

'Candidatus Phytoplasma wodyetiae’, a new taxon associated with yellow decline disease of foxtail palm (Wodyetia bifurcata) in Malaysia
by: Naderali, Neda, et al.
Published: (2017)

Hepatitis C genotyping in hemodialysis patients and the development of genotyping method based on TaqMan Probe Real Time (PCR)
by: Hamzah, Hairul Aini, et al.
Published: (2010)

Detection of hepatitis B virus core antigen by phage display mediated TaqMan real-time immuno-PCR
by: Monjezi, Razieh, et al.
Published: (2013)

Detection, characterization and antimicrobial activity of nano silver against coconut yellow decline phytoplasmas in coconut and ornamental palms in Malaysia
by: Naderali, Neda
Published: (2015)

Development of TaqMan-based real-time RT-PCR assay based on N gene for the quantitative detection of feline morbillivirus
by: Makhtar, Siti Tasnim, et al.
Published: (2021)

'Candidatus Phytoplasma malaysianum', a novel taxon associated with virescence and phyllody of Madagascar periwinkle (Catharanthus roseus).
by: Nejat, Naghmeh, et al.
Published: (2013)

High throughput molecular confirmation of β-thalassemia mutations using novel TaqMan probes
by: Kho, Siew Leng, et al.
Published: (2013)

TaqMan real-time PCR assay for relative quantitation of white spot syndrome virus infection in Penaeus monodon Fabricius exposed to ammonia.
by: M., Fouzi, et al.
Published: (2010)

MethyLight TaqMan® Assay for quantification of DNA methylation of Reelin (RELN) gene in schizophrenia: preliminary data
by: Rahim, Mohd Nabil Fikri, et al.
Published: (2015)

MethyLight TaqMan® assay for quantification of DNA methylation of Reelin (RELN) gene in schizophrenia: preliminary data
by: Rahim, Mohd Nabil Fikri, et al.
Published: (2015)

Detection of porcine adulteration in cosmetic cream formulation via TaqMan probe real-time polymerase chain reaction
by: Mustafa, Suhana
Published: (2020)

Sequence analysis of RNA-dependent RNA polymerase and development of a TaqMan real-time RT-PCR assay for detection of Macrobrachium rosenbergii nodavirus in giant freshwater prawns
by: Mohammadi, Zeinabsadat
Published: (2012)

Specific and straightforward molecular investigation of β-thalassemia mutations in the Malaysian Malays and Chinese using direct TaqMan genotyping assays
by: Kho, Siew Leng, et al.
Published: (2013)

Spiroplasma citri : a wide host range phytopathogen.
by: Nejat, Naghmeh, et al.
Published: (2011)

Expression patterns of genes involved in the defense and stress response of Spiroplasma citri infected Madagascar Periwinkle Catharanthus roseus.
by: Nejat, Naghmeh, et al.
Published: (2012)

'Candidatus phytoplasma palmicola’: a novel taxon associated with a lethal yellowing-type disease (LYD) of coconut ( Cocos nucifera L.) in Mozambique
by: Harrison, Nigel A., et al.
Published: (2014)

Molecular methods for detecting the coconut lethal disease (LD) phytoplasma in Tanzania
by: Mpunami, Anatolia A.
Published: (1997)

The effect of variable taq Polymerase concentrations in Polymerase chain reaction (PCR) / Zanura Adzhari
by: Zanura, Adzhari
Published: (2007)

Comparison of real-time PCR, conventional PCR and RT-lamp for the detection of Coconut Cadang-Cadang Viroid variant in oil palm
by: Roslan, Nur Diyana, et al.
Published: (2023)

Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia
by: Darshanie, Tennakon Mudiyanselage Nadika, et al.
Published: (2020)

Improvement of phytoplasma diagnostic techniques
by: Aljafer, Naofel
Published: (2016)

Development of improved methods for phytoplasma diagnostics
by: Al-Jaf, Bryar Salar Kamal
Published: (2017)

A taqman duplex quantitative PCR method for detecting Klebsiella pneumoniae has been developed based on pan-genome analysis
by: Li, Zenghui, et al.
Published: (2025)

Coconut cadang-cadang viroid and coconut tinangaja viroid
by: Vadamalai, Ganesan, et al.
Published: (2017)

Refinement of the taxonomic structure of 16SrXI and 16SrXIV phytoplasmas of gramineous plants using multilocus sequence typing
by: Abeysinghe, Saman, et al.
Published: (2016)

Development of taqman-based real-time rt-pcr assay for quantitative detection of feline morbillivirus (feMV) and phylogenetic analysis of Malaysian feMV isolates
by: Makhtar, Siti Tasnim
Published: (2021)