Development of a PCR primer and a marker band for detection of E. coli from various sources based on arbitrary primer set.
Although, PCR methods aimed on the detection of genes associated with the pathogenicity of Escherichia coli have been reported, tests allowing the direct identification of this serotype are rare. In this study the Random Amplified Polymorphic DNA (RAPD) fingerprinting technique allowed genetic diver...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Medwell Journals
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13994/ http://psasir.upm.edu.my/id/eprint/13994/1/Development%20of%20a%20PCR%20primer%20and%20a%20marker%20band%20for%20detection%20of%20E.pdf |
| _version_ | 1848842269909581824 |
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| author | Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdul Rahim, Raha Ishak, Zamri |
| author_facet | Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdul Rahim, Raha Ishak, Zamri |
| author_sort | Abdullah Al-Haj, Nagi Ahmed |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Although, PCR methods aimed on the detection of genes associated with the pathogenicity of Escherichia coli have been reported, tests allowing the direct identification of this serotype are rare. In this study the Random Amplified Polymorphic DNA (RAPD) fingerprinting technique allowed genetic diversity assessment of 25 E. coli isolates of various sources. A highly significant finding from the DNA fingerprinting is the display of a predominant band at a size of 308 bp when arbitrary OPAE-10 primer was used. After sequencing this fragment primer called secD was designed to be used as PCR primer. secD primer pairs was highly specific to detect all isolates including E. coli O157: H7. |
| first_indexed | 2025-11-15T07:56:27Z |
| format | Article |
| id | upm-13994 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:56:27Z |
| publishDate | 2008 |
| publisher | Medwell Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-139942015-12-03T02:58:25Z http://psasir.upm.edu.my/id/eprint/13994/ Development of a PCR primer and a marker band for detection of E. coli from various sources based on arbitrary primer set. Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdul Rahim, Raha Ishak, Zamri Although, PCR methods aimed on the detection of genes associated with the pathogenicity of Escherichia coli have been reported, tests allowing the direct identification of this serotype are rare. In this study the Random Amplified Polymorphic DNA (RAPD) fingerprinting technique allowed genetic diversity assessment of 25 E. coli isolates of various sources. A highly significant finding from the DNA fingerprinting is the display of a predominant band at a size of 308 bp when arbitrary OPAE-10 primer was used. After sequencing this fragment primer called secD was designed to be used as PCR primer. secD primer pairs was highly specific to detect all isolates including E. coli O157: H7. Medwell Journals 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13994/1/Development%20of%20a%20PCR%20primer%20and%20a%20marker%20band%20for%20detection%20of%20E.pdf Abdullah Al-Haj, Nagi Ahmed and Shamsudin, Mariana Nor and Abdul Rahim, Raha and Ishak, Zamri (2008) Development of a PCR primer and a marker band for detection of E. coli from various sources based on arbitrary primer set. Research Journal of Biological Sciences, 3 (11). pp. 1327-1332. ISSN 1815-8846; ESSN: 1993-6087 http://medwelljournals.com/journalhome.php?jid=1815-8846 English |
| spellingShingle | Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdul Rahim, Raha Ishak, Zamri Development of a PCR primer and a marker band for detection of E. coli from various sources based on arbitrary primer set. |
| title | Development of a PCR primer and a marker band for detection of E. coli from various sources based on arbitrary primer set. |
| title_full | Development of a PCR primer and a marker band for detection of E. coli from various sources based on arbitrary primer set. |
| title_fullStr | Development of a PCR primer and a marker band for detection of E. coli from various sources based on arbitrary primer set. |
| title_full_unstemmed | Development of a PCR primer and a marker band for detection of E. coli from various sources based on arbitrary primer set. |
| title_short | Development of a PCR primer and a marker band for detection of E. coli from various sources based on arbitrary primer set. |
| title_sort | development of a pcr primer and a marker band for detection of e. coli from various sources based on arbitrary primer set. |
| url | http://psasir.upm.edu.my/id/eprint/13994/ http://psasir.upm.edu.my/id/eprint/13994/ http://psasir.upm.edu.my/id/eprint/13994/1/Development%20of%20a%20PCR%20primer%20and%20a%20marker%20band%20for%20detection%20of%20E.pdf |