Detection of virulence genes and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) analysis among raw vegetables isolates of Campylobacter jejuni
A total of 20 (n=20) raw vegetable isolates of Campylobacter jejuni were examined for their virulence genes and genetic diversity by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) analysis. All raw vegetable isolates of C. jejuni were encoded cadF and ceuE genes...
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13940/ http://psasir.upm.edu.my/id/eprint/13940/1/13940.pdf |
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| author | Abd. Mutalib, Sahilah Tuan Soh, Tuan Suraya Ismail, Noraida Ariffin, Ahmad Azuhairi Chai, Lay Ching Radu, Son |
| author_facet | Abd. Mutalib, Sahilah Tuan Soh, Tuan Suraya Ismail, Noraida Ariffin, Ahmad Azuhairi Chai, Lay Ching Radu, Son |
| author_sort | Abd. Mutalib, Sahilah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A total of 20 (n=20) raw vegetable isolates of Campylobacter jejuni were examined for their virulence genes and genetic diversity by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) analysis. All raw vegetable isolates of C. jejuni were encoded cadF and ceuE genes. None of the C. jejuni isolates was cdtA positive. Whereas, 12 (12/20) isolates were positive for cdtB and 6 (6/20) were positive for cdtC. All the 20 isolates of C. jejuni were subtyped and they produced 18 ERIC-PCR profiles namely E1 to E18. Dendrogram performed from cluster analysis showed that the 20 isolates were grouped into 2 major clusters, cluster I and II indicating the high level of local geographical genetic variation. The detection of virulence genes among C. jejuni strains isolated from raw vegetables raised concern on the threat of campylobacteriosis. |
| first_indexed | 2025-11-15T07:56:13Z |
| format | Article |
| id | upm-13940 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:56:13Z |
| publishDate | 2010 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-139402015-09-25T06:59:05Z http://psasir.upm.edu.my/id/eprint/13940/ Detection of virulence genes and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) analysis among raw vegetables isolates of Campylobacter jejuni Abd. Mutalib, Sahilah Tuan Soh, Tuan Suraya Ismail, Noraida Ariffin, Ahmad Azuhairi Chai, Lay Ching Radu, Son A total of 20 (n=20) raw vegetable isolates of Campylobacter jejuni were examined for their virulence genes and genetic diversity by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) analysis. All raw vegetable isolates of C. jejuni were encoded cadF and ceuE genes. None of the C. jejuni isolates was cdtA positive. Whereas, 12 (12/20) isolates were positive for cdtB and 6 (6/20) were positive for cdtC. All the 20 isolates of C. jejuni were subtyped and they produced 18 ERIC-PCR profiles namely E1 to E18. Dendrogram performed from cluster analysis showed that the 20 isolates were grouped into 2 major clusters, cluster I and II indicating the high level of local geographical genetic variation. The detection of virulence genes among C. jejuni strains isolated from raw vegetables raised concern on the threat of campylobacteriosis. Faculty of Food Science and Technology, Universiti Putra Malaysia 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13940/1/13940.pdf Abd. Mutalib, Sahilah and Tuan Soh, Tuan Suraya and Ismail, Noraida and Ariffin, Ahmad Azuhairi and Chai, Lay Ching and Radu, Son (2010) Detection of virulence genes and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) analysis among raw vegetables isolates of Campylobacter jejuni. International Food Research Journal, 17 (3). pp. 681-690. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/17%20%2803%29%202010/IFRJ-2010-681-690%20Sahilah.pdf |
| spellingShingle | Abd. Mutalib, Sahilah Tuan Soh, Tuan Suraya Ismail, Noraida Ariffin, Ahmad Azuhairi Chai, Lay Ching Radu, Son Detection of virulence genes and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) analysis among raw vegetables isolates of Campylobacter jejuni |
| title | Detection of virulence genes and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) analysis among raw vegetables isolates of Campylobacter jejuni |
| title_full | Detection of virulence genes and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) analysis among raw vegetables isolates of Campylobacter jejuni |
| title_fullStr | Detection of virulence genes and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) analysis among raw vegetables isolates of Campylobacter jejuni |
| title_full_unstemmed | Detection of virulence genes and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) analysis among raw vegetables isolates of Campylobacter jejuni |
| title_short | Detection of virulence genes and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) analysis among raw vegetables isolates of Campylobacter jejuni |
| title_sort | detection of virulence genes and enterobacterial repetitive intergenic consensus-pcr (eric-pcr) analysis among raw vegetables isolates of campylobacter jejuni |
| url | http://psasir.upm.edu.my/id/eprint/13940/ http://psasir.upm.edu.my/id/eprint/13940/ http://psasir.upm.edu.my/id/eprint/13940/1/13940.pdf |