Detection of Shiga toxin 1 and 2 (stx1 and stx2) genes in Escherichia coli O157:H7 isolated from retail beef in Malaysia by multiplex polymerase chain reaction (PCR)

QR Code

Detection of Shiga toxin 1 and 2 (stx1 and stx2) genes in Escherichia coli O157:H7 isolated from retail beef in Malaysia by multiplex polymerase chain reaction (PCR)

Twenty (n=20) beef isolates of Escherichia coli O157:H7 were examined for the detection of Shiga- toxin 1 and 2 (stx1 and stx2) genes by multiplex polymerase chain reaction (PCR) and characterized using Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) fingerprinting. All isolate...

Full description

Bibliographic Details
Main Authors:	Abd Mutalib, Sahilah, Hamzah, Nor' Aishah, Ismail, Noraida, Ariffin, Ahmad Azuhairi
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2010
Online Access:	http://psasir.upm.edu.my/id/eprint/13938/ http://psasir.upm.edu.my/id/eprint/13938/1/Detection%20of%20Shiga%20Toxin%201%20and%202.pdf

Similar Items

Detection of shiga toxin 1 and 2 (stx1 and stx2) genes in escherichia coli 0157: H7 isolated from retail beef in Malaysia by multiplex polymerase chain reaction (PCR)
by: A.M. Sahilah,, et al.
Published: (2010)

The use of loop AMP in detection of Shiga-toxin (stx) gene among escherichia coli from aquaculture and other environments
by: Chai,, Siong Kiat.
Published: (2013)

Investigation of stx2 eae+ Escherichia coli O157:H7 in beef imported from Malaysia to Thailand
by: Sukhumungoon, Pharanai, et al.
Published: (2011)

Presence of Shiga-toxin producing Escherichia coli and Escherichia coli O157: H7 in local beef and Indian buffalo meat
by: Kwan, Sin Yen
Published: (2018)

Quantitative detection and characterization of Shiga toxin-producing Escherichia coli O157 and non-O157 in raw vegetables by MPN-PCR in Malaysia
by: Loo, Yuet Ying, et al.
Published: (2013)

Occurrence of Escherichia coli harbouring stx genes in popiah, a Malaysian street food
by: Nillian, Elexson, et al.
Published: (2017)

Occurrence of non-O157 Shiga toxin-producing Escherichia coli in healthy cattle and goats and distribution of virulence genes among isolates
by: Tayzar, A. C., et al.
Published: (2013)

Characterization of Shiga Toxigenic Escherichia coli O157 and Non-O157 Isolates from Ruminant Feces in Malaysia
by: Perera, A., et al.
Published: (2015)

Detection and molecular characterization of shiga-like toxin producing escherichia coli and escherichia coli 0157 : H7 isolated from raw beef marketed in east Malaysia
by: Chang, Pheh Ping
Published: (2003)

Analysis of Escherichia coli O157:H7 Isolates from Beef by RAPD-PCR and Plasmid Profiles
by: Son, Radu, et al.
Published: (2000)

Optimization of multiplex PCR conditions for rapid detection of Escherichia coli O157:H7 virulence genes
by: Ranjit Singh, Jeshveen Singh, et al.
Published: (2012)

Shiga toxin Escherichia coli survival in different blending ratio of fresh pineapple-mango juice blends
by: Kamarul Zaman, Amanina Amani, et al.
Published: (2017)

Monte Carlo simulation of 6 MV flattening filter free photon beam of TrueBeam STx LINAC at Songklanagarind Hospital
by: M. Arif Efendi,, et al.
Published: (2017)

Isolation of non-0157 shiga toxin-producing escherichia coli from cattle and goats and detection of their virulence genes
by: Cho, Tay Zar Aye
Published: (2011)

Detection of Escherichia coli O157:H7 in the beef marketed in Malaysia
by: Radu, Son, et al.
Published: (1998)

Plasmid Profiling and Antibiotic Resistance of Escherichia Coli and Escherichia Coli O157 Strains
by: Abd. Mutalib, Sahilah
Published: (1997)

Detection of Escherichia coli O157:H7 by multiplex PCR and their characterization by plasmid profiling, antimicrobial resistance, RAPD and PFGE analyses
by: Radu, Son, et al.
Published: (2001)

Risk of Escherichia coli O157:H7 infection linked to the consumption of beef
by: Premarathne, Jayasekara Mudiyanselage Krishanthi Jayarukshi Kumari, et al.
Published: (2017)

DNA profiling among egg and beef meat isolates of Escherichia coli by enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and random amplified polymorphic DNA-PCR (RAPD-PCR)
by: Abd Mutalib, Sahilah, et al.
Published: (2010)

Rapid isolation and detection of Escherichia coli O157H7 by use of rainbow agar O157 and PCR assay
by: Son, Radu, et al.
Published: (2000)

Isolation of verotoxin-producing Escherichia coli associated with diarrhoea in Malaysia containing plasmids showing homology with biotinylated Shiga-like toxin DNA gene probes
by: Radu, Son, et al.
Published: (1996)

Detection of citrus viroids by multiplex reverse transcription polymerase chain reaction (RT-PCR)
by: Roslin, Nor Athirah
Published: (2017)

Development of a Specfic Pcr-Based Assay for the Detection of Escherichia Coli O157: H7
by: Gurnam Singh, Sushil Kaur
Published: (2004)

Identification and Detection of Shigella Species from Wildlife Using Multiplex Polymerase Chain Reaction (mPCR)
by: Wong, Ngah Kiat
Published: (2010)

Real-time PCR for detection of fliC gene of E. coli O157:H7 in beef and chicken meat
by: Mohd Saad, Suria, et al.
Published: (2012)

Comparative analysis of attachment of shiga-toxigenic Escherichia coli and Salmonella strains to cultured HT-29 and caco-2 cell lines
by: Mellor, G., et al.
Published: (2009)

Application of the Multiplex Polymerase Chain Reaction (M-PCR) for the Screening of Vibrio spp. from Rivers in Kuching, Sarawak
by: Micky, Vincent, et al.
Published: (2015)

A multiplex polymerase chain reaction (mPCR) assay for detection of salmonella typhi and salmonella paratyphi A
by: Daun, Kenny AK
Published: (2011)

Development of multiplex polymerase chain reaction (PCR) assay for the simultaneous detection of vancomycin and linezolid resistant genes in enterococcus
by: Wada, Yusuf
Published: (2022)

Prevalence and molecular characterisation of Escherichia coli O157: H7 and non-O157
by: Rusul, Gulam, et al.
Published: (1999)

Species identification from selected beef patty brands using polymerase chain reaction (PCR) / Syafiqah Alia Zainudin
by: Zainudin, Syafiqah Alia
Published: (2017)

Diet halalan toyyiban: porcine blood plasms detection in chicken meatball by conventional polymerase chain reaction (PCR) analysis
by: Ismail, Norfadzilah, et al.
Published: (2018)

Antibiotic resistance and molecular typing among cockle (Anadara granosa) strains of Vibrio parahaemolyticus by polymerase chain reaction (PCR)-based analysis
by: Abd Mutalib, Sahilah, et al.
Published: (2014)

Incidence of Escherichia coli O157:H7 in Thailand
by: Sukhumungoon, Pharanai
Published: (2015)

Double gene targeting multiplex polymerase chain reaction–restriction fragment length polymorphism assay discriminates beef, buffalo, and pork substitution in frankfurter products
by: Hossain, Motalib A.Motalib, et al.
Published: (2016)

The effect of variable taq Polymerase concentrations in Polymerase chain reaction (PCR) / Zanura Adzhari
by: Zanura, Adzhari
Published: (2007)

Molecular Characterisation Of Escherichia Coli Serogroup O 157:H7
by: Mohd Dzomir, Ahmad Zainuri
Published: (1998)

A New Nested Allele-Specific Multiplex Polymerase Chain Reaction Method for Haplotyping of VKORC1 Gene to Predict Warfarin Sensitivity
by: Yung, An Chua, et al.
Published: (2014)

Distribution and quantification of escherichia coli and escherichia coli O157 : H7 in organic Vegetables at Farm Level
by: Lim, Poh Yiin
Published: (2013)

Occurrence and Quantification of escherichia coli and escherichia coli O157:H7 on Conventional Vegetables at Farm Level
by: Sea, Lillian Shun Yi
Published: (2013)