Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction.
Subtype-specific multiplex reverse transcription-polymerase chain reaction (RT-PCR) was developed to simultaneously detect three subtypes (H5, H7 and H9) of avian influenza virus (AIV) type A. The sensitivity of the multiplex RT-PCR was evaluated and compared to that of RT-PCR–enzyme-linked immunoso...
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| Format: | Article |
| Language: | English English |
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Elsevier
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13933/ http://psasir.upm.edu.my/id/eprint/13933/1/Detection%20of%20H5.pdf |
| _version_ | 1848842252686721024 |
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| author | B., Chaharaein Omar, Abdul Rahman Ideris, Aini Yusoff, Khatijah Syed Hassan, Sharifah |
| author_facet | B., Chaharaein Omar, Abdul Rahman Ideris, Aini Yusoff, Khatijah Syed Hassan, Sharifah |
| author_sort | B., Chaharaein |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Subtype-specific multiplex reverse transcription-polymerase chain reaction (RT-PCR) was developed to simultaneously detect three subtypes (H5, H7 and H9) of avian influenza virus (AIV) type A. The sensitivity of the multiplex RT-PCR was evaluated and compared to that of RT-PCR–enzyme-linked immunosorbent assay (ELISA) and conventional RT-PCR. While the sensitivity of the multiplex RT-PCR is as sensitive as the conventional RT-PCR, it is 10 times less sensitive than RT-PCR–ELISA. The multiplex RT-PCR is also as sensitive as the virus isolation method in detecting H9N2 from tracheal samples collected at day 3 and 5 post inoculation. Hence, the developed multiplex RT-PCR assay is a rapid, sensitive and specific assay for detecting of AIV subtypes. |
| first_indexed | 2025-11-15T07:56:11Z |
| format | Article |
| id | upm-13933 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:56:11Z |
| publishDate | 2009 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-139332015-09-02T03:32:56Z http://psasir.upm.edu.my/id/eprint/13933/ Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction. B., Chaharaein Omar, Abdul Rahman Ideris, Aini Yusoff, Khatijah Syed Hassan, Sharifah Subtype-specific multiplex reverse transcription-polymerase chain reaction (RT-PCR) was developed to simultaneously detect three subtypes (H5, H7 and H9) of avian influenza virus (AIV) type A. The sensitivity of the multiplex RT-PCR was evaluated and compared to that of RT-PCR–enzyme-linked immunosorbent assay (ELISA) and conventional RT-PCR. While the sensitivity of the multiplex RT-PCR is as sensitive as the conventional RT-PCR, it is 10 times less sensitive than RT-PCR–ELISA. The multiplex RT-PCR is also as sensitive as the virus isolation method in detecting H9N2 from tracheal samples collected at day 3 and 5 post inoculation. Hence, the developed multiplex RT-PCR assay is a rapid, sensitive and specific assay for detecting of AIV subtypes. Elsevier 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13933/1/Detection%20of%20H5.pdf B., Chaharaein and Omar, Abdul Rahman and Ideris, Aini and Yusoff, Khatijah and Syed Hassan, Sharifah (2009) Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction. Microbiological Research, 164 (2). pp. 174-179. ISSN 0944-5013 10.1016/j.micres.2007.01.001 English |
| spellingShingle | B., Chaharaein Omar, Abdul Rahman Ideris, Aini Yusoff, Khatijah Syed Hassan, Sharifah Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction. |
| title | Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction. |
| title_full | Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction. |
| title_fullStr | Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction. |
| title_full_unstemmed | Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction. |
| title_short | Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction. |
| title_sort | detection of h5, h7 and h9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction. |
| url | http://psasir.upm.edu.my/id/eprint/13933/ http://psasir.upm.edu.my/id/eprint/13933/ http://psasir.upm.edu.my/id/eprint/13933/1/Detection%20of%20H5.pdf |