Detection and differentiation of velogenic and lentogenic Newcastle disease viruses using SYBR Green I real-time PCR with nucleocapsid gene-specific primers

Detection and differentiation of velogenic and lentogenic Newcastle disease viruses using SYBR Green I real-time PCR with nucleocapsid gene-specific primers

SYBR Green I real-time PCR was developed for detection and differentiation of Newcastle disease virus (NDV). Primers based on the nucleocapsid (NP) gene were designed to detect specific sequence of velogenic strains and lentogenic/vaccine strains, respectively. The assay was developed and tested wit...

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Bibliographic Details
Main Authors: Tan, Sheau Wei, Ideris, Aini, Omar, Abdul Rahman, Mohd Yusoff, Khatijah, Bejo, Mohd Hair
Format: Article
Language:English
Published: Elsevier 2009
Online Access:http://psasir.upm.edu.my/id/eprint/13926/
http://psasir.upm.edu.my/id/eprint/13926/1/Detection%20and%20differentiation%20of%20velogenic%20and%20lentogenic%20Newcastle%20disease%20viruses%20using%20SYBR%20Green%20I%20real-time%20PCR%20with%20nucleocapsid%20gene-specific%20primers.pdf

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