Crystallization and preliminary X-ray crystallographic analysis of highly thermostable L2 lipase from the newly isolated Bacillus sp. L2

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Crystallization and preliminary X-ray crystallographic analysis of highly thermostable L2 lipase from the newly isolated Bacillus sp. L2

Purified thermostable recombinant L2 lipase from Bacillus sp. L2 was crystallized by the counter-diffusion method using 20% PEG 6000, 50 mM MES pH 6.5 and 50 mM NaCl as precipitant. X-ray diffraction data were collected to 2.7 Å resolution using an in-house Bruker X8 PROTEUM single-crystal diffracto...

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Bibliographic Details
Main Authors:	Mohd Shariff, Fairolniza, Raja Abdul Rahman, Raja Noor Zaliha, Mohamad Ali, Mohd Shukuri, Leow, Adam Thean Chor, Basri, Mahiran, Salleh, Abu Bakar
Format:	Article
Language:	English
Published:	International Union of Crystallography 2010
Online Access:	http://psasir.upm.edu.my/id/eprint/13836/ http://psasir.upm.edu.my/id/eprint/13836/1/Crystallization%20and%20preliminary%20X.pdf

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