CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.
Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflamm...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Nature Publishing Group
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13817/ http://psasir.upm.edu.my/id/eprint/13817/1/CpG.pdf |
| _version_ | 1848842218849173504 |
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| author | Hyde, Stephen C. Pringle, Ian A. Abdullah, Syahril Lawton, Anna E. Davies, Lee A. Varathalingam, Anusha Nunez-Alonso, Graciela A. Green, Anne Marie Bazzani, Reto P. Sumner-Jones, Stephanie G. Chan, Mario Hong, Yu Li Yew, Nelson S. Cheng, Seng H. Boyd, Christopher A. Davies, Jane C. Griesenbach, Uta Porteous, David John Sheppard, David N. Munkonge, Felix M. Alton, Eric W. F. W. Gill, Deborah R. |
| author_facet | Hyde, Stephen C. Pringle, Ian A. Abdullah, Syahril Lawton, Anna E. Davies, Lee A. Varathalingam, Anusha Nunez-Alonso, Graciela A. Green, Anne Marie Bazzani, Reto P. Sumner-Jones, Stephanie G. Chan, Mario Hong, Yu Li Yew, Nelson S. Cheng, Seng H. Boyd, Christopher A. Davies, Jane C. Griesenbach, Uta Porteous, David John Sheppard, David N. Munkonge, Felix M. Alton, Eric W. F. W. Gill, Deborah R. |
| author_sort | Hyde, Stephen C. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (≥56 d) in vivo transgene expression in the absence of lung inflammation. |
| first_indexed | 2025-11-15T07:55:39Z |
| format | Article |
| id | upm-13817 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:55:39Z |
| publishDate | 2008 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-138172015-09-17T00:37:15Z http://psasir.upm.edu.my/id/eprint/13817/ CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. Hyde, Stephen C. Pringle, Ian A. Abdullah, Syahril Lawton, Anna E. Davies, Lee A. Varathalingam, Anusha Nunez-Alonso, Graciela A. Green, Anne Marie Bazzani, Reto P. Sumner-Jones, Stephanie G. Chan, Mario Hong, Yu Li Yew, Nelson S. Cheng, Seng H. Boyd, Christopher A. Davies, Jane C. Griesenbach, Uta Porteous, David John Sheppard, David N. Munkonge, Felix M. Alton, Eric W. F. W. Gill, Deborah R. Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (≥56 d) in vivo transgene expression in the absence of lung inflammation. Nature Publishing Group 2008-05 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13817/1/CpG.pdf Hyde, Stephen C. and Pringle, Ian A. and Abdullah, Syahril and Lawton, Anna E. and Davies, Lee A. and Varathalingam, Anusha and Nunez-Alonso, Graciela A. and Green, Anne Marie and Bazzani, Reto P. and Sumner-Jones, Stephanie G. and Chan, Mario and Hong, Yu Li and Yew, Nelson S. and Cheng, Seng H. and Boyd, Christopher A. and Davies, Jane C. and Griesenbach, Uta and Porteous, David John and Sheppard, David N. and Munkonge, Felix M. and Alton, Eric W. F. W. and Gill, Deborah R. (2008) CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. Nature Biotechnology, 26 (5). pp. 549-551. ISSN 1087-0156; ESSN: 1546-1696 http://www.nature.com/nbt/index.html 10.1038/nbt1399 English |
| spellingShingle | Hyde, Stephen C. Pringle, Ian A. Abdullah, Syahril Lawton, Anna E. Davies, Lee A. Varathalingam, Anusha Nunez-Alonso, Graciela A. Green, Anne Marie Bazzani, Reto P. Sumner-Jones, Stephanie G. Chan, Mario Hong, Yu Li Yew, Nelson S. Cheng, Seng H. Boyd, Christopher A. Davies, Jane C. Griesenbach, Uta Porteous, David John Sheppard, David N. Munkonge, Felix M. Alton, Eric W. F. W. Gill, Deborah R. CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. |
| title | CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. |
| title_full | CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. |
| title_fullStr | CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. |
| title_full_unstemmed | CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. |
| title_short | CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. |
| title_sort | cpg-free plasmids confer reduced inflammation and sustained pulmonary gene expression. |
| url | http://psasir.upm.edu.my/id/eprint/13817/ http://psasir.upm.edu.my/id/eprint/13817/ http://psasir.upm.edu.my/id/eprint/13817/ http://psasir.upm.edu.my/id/eprint/13817/1/CpG.pdf |