Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid
To construct an expression vector for Lactococcus lactis, the EmPMT fragment which contained the erythromycin resistance gene, P32 promoter, multiple cloning site (MCS) and terminator (T) was subcloned into the small cryptic plasmid pAR141. The resulting vector, designated as pAR1411, was found to b...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Academic Journals
2009
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/13757/ http://psasir.upm.edu.my/id/eprint/13757/1/13757.pdf |
| _version_ | 1848842201294962688 |
|---|---|
| author | Hooi, Wei Yeng Shamsudin, Mariana Nor Abdul Rahim, Raha |
| author_facet | Hooi, Wei Yeng Shamsudin, Mariana Nor Abdul Rahim, Raha |
| author_sort | Hooi, Wei Yeng |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | To construct an expression vector for Lactococcus lactis, the EmPMT fragment which contained the erythromycin resistance gene, P32 promoter, multiple cloning site (MCS) and terminator (T) was subcloned into the small cryptic plasmid pAR141. The resulting vector, designated as pAR1411, was found to be stably maintained in L. lactis MG1363 after transformation for at least 100 generations under non-selective conditions. The vector was also demonstrated to be able to express the gene coding for chloramphenicol acetyltransferase (cat) in L. lactis. |
| first_indexed | 2025-11-15T07:55:22Z |
| format | Article |
| id | upm-13757 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:55:22Z |
| publishDate | 2009 |
| publisher | Academic Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-137572017-11-13T02:37:56Z http://psasir.upm.edu.my/id/eprint/13757/ Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid Hooi, Wei Yeng Shamsudin, Mariana Nor Abdul Rahim, Raha To construct an expression vector for Lactococcus lactis, the EmPMT fragment which contained the erythromycin resistance gene, P32 promoter, multiple cloning site (MCS) and terminator (T) was subcloned into the small cryptic plasmid pAR141. The resulting vector, designated as pAR1411, was found to be stably maintained in L. lactis MG1363 after transformation for at least 100 generations under non-selective conditions. The vector was also demonstrated to be able to express the gene coding for chloramphenicol acetyltransferase (cat) in L. lactis. Academic Journals 2009-11-02 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13757/1/13757.pdf Hooi, Wei Yeng and Shamsudin, Mariana Nor and Abdul Rahim, Raha (2009) Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid. African Journal of Biotechnology, 8 (21). art. no. 84F18C88334. pp. 5621-5626. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/84F18C88334 |
| spellingShingle | Hooi, Wei Yeng Shamsudin, Mariana Nor Abdul Rahim, Raha Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid |
| title | Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid |
| title_full | Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid |
| title_fullStr | Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid |
| title_full_unstemmed | Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid |
| title_short | Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid |
| title_sort | construction of an expression vector for lactococcus lactis based on an indigenous cryptic plasmid |
| url | http://psasir.upm.edu.my/id/eprint/13757/ http://psasir.upm.edu.my/id/eprint/13757/ http://psasir.upm.edu.my/id/eprint/13757/1/13757.pdf |