Cross-species amplification study of Tor douronensis and Tor tambroides using microsatellites from other cyprinids
This study examined twenty six microsatellite primers developed from three cyprinid fishes (Cyprinus carpio, Barbus barbus and Barbonymus gonionotus) in two indigenous mahseer, Tor douronensis and T. tambroides. A total of 10 (38%) and 12 (46%) primers were successfully amplified producing four and...
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| Format: | Article |
| Language: | English |
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Faculty of Resource Science and Technology, Universiti Malaysia Sarawak
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13704/ http://psasir.upm.edu.my/id/eprint/13704/1/Cross-species%20amplification%20study%20of%20Tor%20douronensis%20and%20Tor%20tambroides%20using%20microsatellites%20from%20other%20cyprinids.pdf |
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| author | Esa, Yuzine Siraj, Siti Shapor A. Rahim, Khairul Adha Daud, Siti Khalijah Ho, Gim Chong Tan, Soon Guan |
| author_facet | Esa, Yuzine Siraj, Siti Shapor A. Rahim, Khairul Adha Daud, Siti Khalijah Ho, Gim Chong Tan, Soon Guan |
| author_sort | Esa, Yuzine |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study examined twenty six microsatellite primers developed from three cyprinid fishes (Cyprinus carpio, Barbus barbus and Barbonymus gonionotus) in two indigenous mahseer, Tor douronensis and T. tambroides. A total of 10 (38%) and 12 (46%) primers were successfully amplified producing four and five polymorphic loci in T. douronensis and T. tambroides, respectively. The number of alleles per locus ranging from 2 to 5 and 2 to 7 in T. douronensis and T. tambroides, respectively. A significant deviation from Hardy-Weinberg equilibrium (HWE) was observed at three loci (Barb37, Barb59 and Barb62) in one or more populations in T. tambroides while two loci (Barb37 and Barb62) were deviated in T. douronensis population of Batang Ai. Bayesian cluster analysis performed with STRUCTURE showed that the most likely K value identified was K = 2 with no evidence of population substructuring, similar to those identified by the UPGMA dendrogram. The low genetic distances among populations were also supported by low interpopulation genetic differences (FST) among pairwise populations in both mahseer. Overall, the identified microsatellite loci exhibit promise for use in fine scale population structure analysis of T. douronensis and T. tambroides natural populations. |
| first_indexed | 2025-11-15T07:55:07Z |
| format | Article |
| id | upm-13704 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:55:07Z |
| publishDate | 2011 |
| publisher | Faculty of Resource Science and Technology, Universiti Malaysia Sarawak |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-137042019-10-24T06:49:44Z http://psasir.upm.edu.my/id/eprint/13704/ Cross-species amplification study of Tor douronensis and Tor tambroides using microsatellites from other cyprinids Esa, Yuzine Siraj, Siti Shapor A. Rahim, Khairul Adha Daud, Siti Khalijah Ho, Gim Chong Tan, Soon Guan This study examined twenty six microsatellite primers developed from three cyprinid fishes (Cyprinus carpio, Barbus barbus and Barbonymus gonionotus) in two indigenous mahseer, Tor douronensis and T. tambroides. A total of 10 (38%) and 12 (46%) primers were successfully amplified producing four and five polymorphic loci in T. douronensis and T. tambroides, respectively. The number of alleles per locus ranging from 2 to 5 and 2 to 7 in T. douronensis and T. tambroides, respectively. A significant deviation from Hardy-Weinberg equilibrium (HWE) was observed at three loci (Barb37, Barb59 and Barb62) in one or more populations in T. tambroides while two loci (Barb37 and Barb62) were deviated in T. douronensis population of Batang Ai. Bayesian cluster analysis performed with STRUCTURE showed that the most likely K value identified was K = 2 with no evidence of population substructuring, similar to those identified by the UPGMA dendrogram. The low genetic distances among populations were also supported by low interpopulation genetic differences (FST) among pairwise populations in both mahseer. Overall, the identified microsatellite loci exhibit promise for use in fine scale population structure analysis of T. douronensis and T. tambroides natural populations. Faculty of Resource Science and Technology, Universiti Malaysia Sarawak 2011 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/13704/1/Cross-species%20amplification%20study%20of%20Tor%20douronensis%20and%20Tor%20tambroides%20using%20microsatellites%20from%20other%20cyprinids.pdf Esa, Yuzine and Siraj, Siti Shapor and A. Rahim, Khairul Adha and Daud, Siti Khalijah and Ho, Gim Chong and Tan, Soon Guan (2011) Cross-species amplification study of Tor douronensis and Tor tambroides using microsatellites from other cyprinids. Borneo Journal of Resource Science and Technology, 1 (1). pp. 14-23. ISSN 2229-9769 http://publisher.unimas.my/ojs/index.php/BJRST/article/view/258 |
| spellingShingle | Esa, Yuzine Siraj, Siti Shapor A. Rahim, Khairul Adha Daud, Siti Khalijah Ho, Gim Chong Tan, Soon Guan Cross-species amplification study of Tor douronensis and Tor tambroides using microsatellites from other cyprinids |
| title | Cross-species amplification study of Tor douronensis and Tor tambroides using microsatellites from other cyprinids |
| title_full | Cross-species amplification study of Tor douronensis and Tor tambroides using microsatellites from other cyprinids |
| title_fullStr | Cross-species amplification study of Tor douronensis and Tor tambroides using microsatellites from other cyprinids |
| title_full_unstemmed | Cross-species amplification study of Tor douronensis and Tor tambroides using microsatellites from other cyprinids |
| title_short | Cross-species amplification study of Tor douronensis and Tor tambroides using microsatellites from other cyprinids |
| title_sort | cross-species amplification study of tor douronensis and tor tambroides using microsatellites from other cyprinids |
| url | http://psasir.upm.edu.my/id/eprint/13704/ http://psasir.upm.edu.my/id/eprint/13704/ http://psasir.upm.edu.my/id/eprint/13704/1/Cross-species%20amplification%20study%20of%20Tor%20douronensis%20and%20Tor%20tambroides%20using%20microsatellites%20from%20other%20cyprinids.pdf |