Comparative evaluation of different cell disruption methods for the release of recombinant hepatitis B core antigen from Escherichia coli
A comparative evaluation of five different cell-disruption methods for the release of recombinant hepatitis B core antigen (HBcAg) from Escherichia coli was investigated. The cell disruption techniques evaluated in this study were high-pressure homogenization, batch-mode bead milling, continuous-rec...
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| Format: | Article |
| Language: | English |
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Korean Society for Biotechnology and Bioengineering
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13664/ http://psasir.upm.edu.my/id/eprint/13664/1/Comparative%20evaluation%20of%20different%20cell%20disruption%20methods%20for%20the%20release%20of%20recombinant%20hepatitis%20B%20core%20antigen%20from%20Escherichia%20coli.pdf |
| _version_ | 1848842174567809024 |
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| author | Ho, Chin Woi Tan, Wen Siang Yap, Wei Boon Ling, Tau Chuan Tey, Beng Ti |
| author_facet | Ho, Chin Woi Tan, Wen Siang Yap, Wei Boon Ling, Tau Chuan Tey, Beng Ti |
| author_sort | Ho, Chin Woi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A comparative evaluation of five different cell-disruption methods for the release of recombinant hepatitis B core antigen (HBcAg) from Escherichia coli was investigated. The cell disruption techniques evaluated in this study were high-pressure homogenization, batch-mode bead milling, continuous-recycling bead milling, ultrasonication, and enzymatic lysis. Continuous-recycling bead milling was found to be the most effective method in terms of operating cost and time. However, the highest degree of cell disruption and amounts of HBcAg were obtained from the high-pressure homogenization process. The direct purification of HBcAg from the unclarified cell disruptate derived from high-pressure homogenization and bead milling techniques, using batch anion-exchange adsorption methods, showed that the conditions of cell disruption have a substantial effect on subsequent protein recovery steps. |
| first_indexed | 2025-11-15T07:54:56Z |
| format | Article |
| id | upm-13664 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:54:56Z |
| publishDate | 2008 |
| publisher | Korean Society for Biotechnology and Bioengineering |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-136642015-09-17T00:24:24Z http://psasir.upm.edu.my/id/eprint/13664/ Comparative evaluation of different cell disruption methods for the release of recombinant hepatitis B core antigen from Escherichia coli Ho, Chin Woi Tan, Wen Siang Yap, Wei Boon Ling, Tau Chuan Tey, Beng Ti A comparative evaluation of five different cell-disruption methods for the release of recombinant hepatitis B core antigen (HBcAg) from Escherichia coli was investigated. The cell disruption techniques evaluated in this study were high-pressure homogenization, batch-mode bead milling, continuous-recycling bead milling, ultrasonication, and enzymatic lysis. Continuous-recycling bead milling was found to be the most effective method in terms of operating cost and time. However, the highest degree of cell disruption and amounts of HBcAg were obtained from the high-pressure homogenization process. The direct purification of HBcAg from the unclarified cell disruptate derived from high-pressure homogenization and bead milling techniques, using batch anion-exchange adsorption methods, showed that the conditions of cell disruption have a substantial effect on subsequent protein recovery steps. Korean Society for Biotechnology and Bioengineering 2008-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13664/1/Comparative%20evaluation%20of%20different%20cell%20disruption%20methods%20for%20the%20release%20of%20recombinant%20hepatitis%20B%20core%20antigen%20from%20Escherichia%20coli.pdf Ho, Chin Woi and Tan, Wen Siang and Yap, Wei Boon and Ling, Tau Chuan and Tey, Beng Ti (2008) Comparative evaluation of different cell disruption methods for the release of recombinant hepatitis B core antigen from Escherichia coli. Biotechnology and Bioprocess Engineering, 13 (5). pp. 577-583. ISSN 1226-8372; ESSN: 1976-3816 10.1007/s12257-008-0020-9 |
| spellingShingle | Ho, Chin Woi Tan, Wen Siang Yap, Wei Boon Ling, Tau Chuan Tey, Beng Ti Comparative evaluation of different cell disruption methods for the release of recombinant hepatitis B core antigen from Escherichia coli |
| title | Comparative evaluation of different cell disruption methods for the release of recombinant hepatitis B core antigen from Escherichia coli |
| title_full | Comparative evaluation of different cell disruption methods for the release of recombinant hepatitis B core antigen from Escherichia coli |
| title_fullStr | Comparative evaluation of different cell disruption methods for the release of recombinant hepatitis B core antigen from Escherichia coli |
| title_full_unstemmed | Comparative evaluation of different cell disruption methods for the release of recombinant hepatitis B core antigen from Escherichia coli |
| title_short | Comparative evaluation of different cell disruption methods for the release of recombinant hepatitis B core antigen from Escherichia coli |
| title_sort | comparative evaluation of different cell disruption methods for the release of recombinant hepatitis b core antigen from escherichia coli |
| url | http://psasir.upm.edu.my/id/eprint/13664/ http://psasir.upm.edu.my/id/eprint/13664/ http://psasir.upm.edu.my/id/eprint/13664/1/Comparative%20evaluation%20of%20different%20cell%20disruption%20methods%20for%20the%20release%20of%20recombinant%20hepatitis%20B%20core%20antigen%20from%20Escherichia%20coli.pdf |