Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice.
Recent in vitro and in vivo studies have demonstrated that zerumbone (ZER) possesses anticancer properties. The main objective of this study was to examine the effectiveness of the combination of ZER and cisplatin (CIS) to treat cervical intraepithelial neoplasia (CIN) in vivo. Microculture tetrazol...
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| Format: | Article |
| Language: | English English |
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Lippincott, Williams & Wilkins
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13631/ http://psasir.upm.edu.my/id/eprint/13631/1/Combination%20of%20zerumbone%20and%20cisplatin%20to%20treat%20cervical%20intraepithelial%20neoplasia%20in%20female%20BALB.pdf |
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| author | Abdul, Ahmad Bustamam Abdelwahab, Siddig Ibrahim Jalinas, Johari Al-Zubairi, Adel Sharaf Taha, Manal Mohamed Elhassan |
| author_facet | Abdul, Ahmad Bustamam Abdelwahab, Siddig Ibrahim Jalinas, Johari Al-Zubairi, Adel Sharaf Taha, Manal Mohamed Elhassan |
| author_sort | Abdul, Ahmad Bustamam |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Recent in vitro and in vivo studies have demonstrated that zerumbone (ZER) possesses anticancer properties. The main objective of this study was to examine the effectiveness of the combination of ZER and cisplatin (CIS) to treat cervical intraepithelial neoplasia (CIN) in vivo. Microculture tetrazolium assay and immunohistochemistry of proliferating cellular nuclear antigen were used to study the antitumor effect of ZER.Prenatally exposed female BALB/c micewere used as a model. The progenies with CIN were injected peritoneally with isotonic sodium chloride solution (positive control), CIS, ZER,and a combination of both compounds. All treated and untreated mice were humanely killed, and serum and cervix were obtained for interleukin 6 analysis and histopathologic studies using hematoxylin and eosin staining, respectively. Zerumbone has revealed an antitumor effect on human cervical cancer cells and downregulates immunoexpression of proliferating cellular nuclear antigen (P < 0.05). In vivo study indicates that ZER at 16 mg/kg and CIS at 10 mg/kg have a regressing effect on CIN. The combination of ZER and CIS also showed similar effectiveness in regressing CIN. Our results indicate that the combination of ZER and CIS has modulated the serum level of interleukin 6 when compared with that in mice treated with isotonic sodium chloride solution (P < 0.05). The effectiveness of combining ZER and CIS could be further explored as a new therapeutic intervention of early precancerous stages of carcinogenesis before the invasive stage begins. |
| first_indexed | 2025-11-15T07:54:48Z |
| format | Article |
| id | upm-13631 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:54:48Z |
| publishDate | 2009 |
| publisher | Lippincott, Williams & Wilkins |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-136312015-11-13T03:45:10Z http://psasir.upm.edu.my/id/eprint/13631/ Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice. Abdul, Ahmad Bustamam Abdelwahab, Siddig Ibrahim Jalinas, Johari Al-Zubairi, Adel Sharaf Taha, Manal Mohamed Elhassan Recent in vitro and in vivo studies have demonstrated that zerumbone (ZER) possesses anticancer properties. The main objective of this study was to examine the effectiveness of the combination of ZER and cisplatin (CIS) to treat cervical intraepithelial neoplasia (CIN) in vivo. Microculture tetrazolium assay and immunohistochemistry of proliferating cellular nuclear antigen were used to study the antitumor effect of ZER.Prenatally exposed female BALB/c micewere used as a model. The progenies with CIN were injected peritoneally with isotonic sodium chloride solution (positive control), CIS, ZER,and a combination of both compounds. All treated and untreated mice were humanely killed, and serum and cervix were obtained for interleukin 6 analysis and histopathologic studies using hematoxylin and eosin staining, respectively. Zerumbone has revealed an antitumor effect on human cervical cancer cells and downregulates immunoexpression of proliferating cellular nuclear antigen (P < 0.05). In vivo study indicates that ZER at 16 mg/kg and CIS at 10 mg/kg have a regressing effect on CIN. The combination of ZER and CIS also showed similar effectiveness in regressing CIN. Our results indicate that the combination of ZER and CIS has modulated the serum level of interleukin 6 when compared with that in mice treated with isotonic sodium chloride solution (P < 0.05). The effectiveness of combining ZER and CIS could be further explored as a new therapeutic intervention of early precancerous stages of carcinogenesis before the invasive stage begins. Lippincott, Williams & Wilkins 2009-08 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13631/1/Combination%20of%20zerumbone%20and%20cisplatin%20to%20treat%20cervical%20intraepithelial%20neoplasia%20in%20female%20BALB.pdf Abdul, Ahmad Bustamam and Abdelwahab, Siddig Ibrahim and Jalinas, Johari and Al-Zubairi, Adel Sharaf and Taha, Manal Mohamed Elhassan (2009) Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice. International Journal of Gynecological Cancer, 19 (6). pp. 1004-1010. ISSN 1048-891X, ESSN: 1525-1438 10.1111/IGC.0b013e3181a83b51 English |
| spellingShingle | Abdul, Ahmad Bustamam Abdelwahab, Siddig Ibrahim Jalinas, Johari Al-Zubairi, Adel Sharaf Taha, Manal Mohamed Elhassan Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice. |
| title | Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice. |
| title_full | Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice. |
| title_fullStr | Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice. |
| title_full_unstemmed | Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice. |
| title_short | Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice. |
| title_sort | combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female balb/c mice. |
| url | http://psasir.upm.edu.my/id/eprint/13631/ http://psasir.upm.edu.my/id/eprint/13631/ http://psasir.upm.edu.my/id/eprint/13631/1/Combination%20of%20zerumbone%20and%20cisplatin%20to%20treat%20cervical%20intraepithelial%20neoplasia%20in%20female%20BALB.pdf |