Chromosome analysis of walking catfish, Clarias spp.

Karyotyping is one of the useful tools for species identification, taxonomy, evolutionary and breeding selection. To date, no information on karyotyping of this fish in the country is documented. The aim of this study was to determine and differentiate the karyotypic structure and fundamental arm nu...

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Main Authors: Siraj, Siti Shapor, Sukardi, Ratiah, Cheah, Cheryl Phaik Imm, Khew, Huai Mei, Vellasamy, Shalini, Panandam, Jothi Malar, Daud, Siti Khalijah
Format: Article
Published: Malaysian Society of Applied Biology 2009
Online Access:http://psasir.upm.edu.my/id/eprint/13593/
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author Siraj, Siti Shapor
Sukardi, Ratiah
Cheah, Cheryl Phaik Imm
Khew, Huai Mei
Vellasamy, Shalini
Panandam, Jothi Malar
Daud, Siti Khalijah
author_facet Siraj, Siti Shapor
Sukardi, Ratiah
Cheah, Cheryl Phaik Imm
Khew, Huai Mei
Vellasamy, Shalini
Panandam, Jothi Malar
Daud, Siti Khalijah
author_sort Siraj, Siti Shapor
building UPM Institutional Repository
collection Online Access
description Karyotyping is one of the useful tools for species identification, taxonomy, evolutionary and breeding selection. To date, no information on karyotyping of this fish in the country is documented. The aim of this study was to determine and differentiate the karyotypic structure and fundamental arm number of the walking catfish; Clarias macrocephalus, Clarias batrachus and Clarias gariepinus. The fish samples ranging from 23–160gm in weight and 16–40cm in length were used to obtain the chromosomes by subjecting to a combination of colchicine and phytohaemagglutinin for arresting a mitotic division. The karyotype of each species was arranged according to size, type and structure such as metacentric (M), submetacentric (SM), subtelocentric (ST) and acrocentric/telocentric (A/T). The diploid chromosome numbers ranged from 2n equal to 54 (Clarias macrocephalus and Clarias batrachus) to 2n equal to 56 (Clarias gariepinus).
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institution Universiti Putra Malaysia
institution_category Local University
last_indexed 2025-11-15T07:54:38Z
publishDate 2009
publisher Malaysian Society of Applied Biology
recordtype eprints
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spelling upm-135932015-06-09T00:50:08Z http://psasir.upm.edu.my/id/eprint/13593/ Chromosome analysis of walking catfish, Clarias spp. Siraj, Siti Shapor Sukardi, Ratiah Cheah, Cheryl Phaik Imm Khew, Huai Mei Vellasamy, Shalini Panandam, Jothi Malar Daud, Siti Khalijah Karyotyping is one of the useful tools for species identification, taxonomy, evolutionary and breeding selection. To date, no information on karyotyping of this fish in the country is documented. The aim of this study was to determine and differentiate the karyotypic structure and fundamental arm number of the walking catfish; Clarias macrocephalus, Clarias batrachus and Clarias gariepinus. The fish samples ranging from 23–160gm in weight and 16–40cm in length were used to obtain the chromosomes by subjecting to a combination of colchicine and phytohaemagglutinin for arresting a mitotic division. The karyotype of each species was arranged according to size, type and structure such as metacentric (M), submetacentric (SM), subtelocentric (ST) and acrocentric/telocentric (A/T). The diploid chromosome numbers ranged from 2n equal to 54 (Clarias macrocephalus and Clarias batrachus) to 2n equal to 56 (Clarias gariepinus). Malaysian Society of Applied Biology 2009 Article PeerReviewed Siraj, Siti Shapor and Sukardi, Ratiah and Cheah, Cheryl Phaik Imm and Khew, Huai Mei and Vellasamy, Shalini and Panandam, Jothi Malar and Daud, Siti Khalijah (2009) Chromosome analysis of walking catfish, Clarias spp. Malaysian Applied Biology Journal, 38 (1). pp. 61-64. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=59:volume-381-june-2009&catid=37:current-view&Itemid=56
spellingShingle Siraj, Siti Shapor
Sukardi, Ratiah
Cheah, Cheryl Phaik Imm
Khew, Huai Mei
Vellasamy, Shalini
Panandam, Jothi Malar
Daud, Siti Khalijah
Chromosome analysis of walking catfish, Clarias spp.
title Chromosome analysis of walking catfish, Clarias spp.
title_full Chromosome analysis of walking catfish, Clarias spp.
title_fullStr Chromosome analysis of walking catfish, Clarias spp.
title_full_unstemmed Chromosome analysis of walking catfish, Clarias spp.
title_short Chromosome analysis of walking catfish, Clarias spp.
title_sort chromosome analysis of walking catfish, clarias spp.
url http://psasir.upm.edu.my/id/eprint/13593/
http://psasir.upm.edu.my/id/eprint/13593/