Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran.
Three infectious bursal disease viruses (IBDVs) were isolated from field outbreaks in IBDV-vaccinated and non-vaccinated layer chicken flocks. Agar gel precipitation test (AGPT), immunoperoxidase staining, transmission electron microscopy (TEM), inoculation into embryonated eggs, and chicken embryo...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
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Academic Electronic Press Ltd.
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13553/ http://psasir.upm.edu.my/id/eprint/13553/1/Characterization%20of%20three%20infectious%20bursal%20disease%20virus%20isolates%20obtained%20from%20layer%20chickens%20in%20Iran.pdf |
| _version_ | 1848842143523667968 |
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| author | M.A., Bahmaninejad Bejo, Mohd Hair Omar, Abdul Rahman Ideris, Aini R., Toroghi |
| author_facet | M.A., Bahmaninejad Bejo, Mohd Hair Omar, Abdul Rahman Ideris, Aini R., Toroghi |
| author_sort | M.A., Bahmaninejad |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Three infectious bursal disease viruses (IBDVs) were isolated from field outbreaks in IBDV-vaccinated and non-vaccinated layer chicken flocks. Agar gel precipitation test (AGPT), immunoperoxidase staining, transmission electron microscopy (TEM), inoculation into embryonated eggs, and chicken embryo fibroblasts (CEFs) confirmed that the isolates were IBDVs. RT-PCR, restriction fragment length polymorphism (RFLP), and phylogenetic analysis demonstrated that the isolates were very virulent IBDV (vvIBDV) and showed a nucleotide sequence similarity of 96.3 to 99.8% in comparison with other vvIBDV strains. It was concluded that the Iranian isolates represented vvIBDV of serotype 1 originating from Europe, Japan, and China. |
| first_indexed | 2025-11-15T07:54:27Z |
| format | Article |
| id | upm-13553 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:54:27Z |
| publishDate | 2008 |
| publisher | Academic Electronic Press Ltd. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-135532015-10-23T07:35:27Z http://psasir.upm.edu.my/id/eprint/13553/ Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran. M.A., Bahmaninejad Bejo, Mohd Hair Omar, Abdul Rahman Ideris, Aini R., Toroghi Three infectious bursal disease viruses (IBDVs) were isolated from field outbreaks in IBDV-vaccinated and non-vaccinated layer chicken flocks. Agar gel precipitation test (AGPT), immunoperoxidase staining, transmission electron microscopy (TEM), inoculation into embryonated eggs, and chicken embryo fibroblasts (CEFs) confirmed that the isolates were IBDVs. RT-PCR, restriction fragment length polymorphism (RFLP), and phylogenetic analysis demonstrated that the isolates were very virulent IBDV (vvIBDV) and showed a nucleotide sequence similarity of 96.3 to 99.8% in comparison with other vvIBDV strains. It was concluded that the Iranian isolates represented vvIBDV of serotype 1 originating from Europe, Japan, and China. Academic Electronic Press Ltd. 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13553/1/Characterization%20of%20three%20infectious%20bursal%20disease%20virus%20isolates%20obtained%20from%20layer%20chickens%20in%20Iran.pdf M.A., Bahmaninejad and Bejo, Mohd Hair and Omar, Abdul Rahman and Ideris, Aini and R., Toroghi (2008) Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran. Acta Virologica, 52 (3). pp. 167-174. ISSN 0001-723X English |
| spellingShingle | M.A., Bahmaninejad Bejo, Mohd Hair Omar, Abdul Rahman Ideris, Aini R., Toroghi Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran. |
| title | Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran. |
| title_full | Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran. |
| title_fullStr | Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran. |
| title_full_unstemmed | Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran. |
| title_short | Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran. |
| title_sort | characterization of three infectious bursal disease virus isolates obtained from layer chickens in iran. |
| url | http://psasir.upm.edu.my/id/eprint/13553/ http://psasir.upm.edu.my/id/eprint/13553/1/Characterization%20of%20three%20infectious%20bursal%20disease%20virus%20isolates%20obtained%20from%20layer%20chickens%20in%20Iran.pdf |