Calcium-induced stabilization of α-amylase against guanidine hydrochloride denaturation

QR Code

Calcium-induced stabilization of α-amylase against guanidine hydrochloride denaturation

Guanidine hydrochloride (GdnHCl) denaturation of native and Ca-depleted Bacillus licheniformis α-amylase (BLA) was investigated both in the absence and presence of 2 mM calcium chloride (CaCl2)using circular dichroism, fluorescence spectroscopy and biological activity. In both states (Cadepleted and...

Full description

Bibliographic Details
Main Authors:	Tan, Cheau Yuaan, Raja Abdul Rahman, Raja Noor Zaliha, Abdul Kadir, Habsah, Tayya, Saad
Format:	Article
Language:	English
Published:	Academic Journals 2010
Online Access:	http://psasir.upm.edu.my/id/eprint/13474/ http://psasir.upm.edu.my/id/eprint/13474/1/13474.pdf

Similar Items

Influence of extrinsic factors on guanidine hydrochloride denaturation of Bacillus licheniformis α-amylase / Salanee Kandandapani
by: Salanee , Kandandapani
Published: (2016)

Conformational destabilization of Bacillus licheniformis α-amylase induced by lysine modification and calcium depletion
by: Tan, Cheau Yuaan, et al.
Published: (2011)

Effect of lysine modification on the conformational stability of a-Amylase / Tan Cheau Yuaan
by: Tan, Cheau Yuaan
Published: (2011)

Coordination chemistry of guanidine derivatives
by: Moore, Charles H. M.
Published: (1987)

Protein engineering of selected residues from conserved sequence regions of a novel Anoxybacillus α-amylase
by: Ranjani, Velayudhan, et al.
Published: (2014)

Native to designed: microbial α-amylases for industrial applications
by: Oslan, Siti Nurbaya, et al.
Published: (2021)

In silico exploration of Isoxazole derivatives of usnic acid: novel therapeutic prospects against α-Amylase for diabetes treatment
by: Roney, Miah, et al.
Published: (2024)

Flavour-saliva interactions: implications of submaxillary mucin and α-amylase
by: Dinu, Vlad
Published: (2020)

Effect of tea polyphenols on α-amylase activity in starch hydrolysis
by: Nurul Nadiah Ismail,, et al.
Published: (2018)

Fluctuation of salivary α-amylase affected by the time change of injection
by: Mohd, Yusri, et al.
Published: (2012)

Stability studies of lincomycin hydrochloride in aqueous solution and intravenous infusion fluids
by: Czarniak, Petra, et al.
Published: (2016)

Alkaline earth complexes of a sterically demanding guanidinate ligand
by: Moxey, Graeme J., et al.
Published: (2015)

Studies On The Heterogenisation Of Sulphonic Acid And Guanidines On Silica And Their Catalytic Activity
by: Batagarawa, Muazu Samaila
Published: (2014)

Cloning and expression of industrial important thermostable amylase gene
by: Sulong, Moohamad Ropaning, et al.
Published: (2008)

Evaluating the Effect of Combined Plant Extracts on α-amylase and α-glucosidase Inhibition Activity as Antidiabetic Agents
by: Munem, Osama A., et al.
Published: (2020)

Potential inhibitors of α-glucosidase and α-amylase enzymes from local fruit waste by solid state fermentation
by: Ahmad Barkat, Anumsima, et al.
Published: (2016)

Molecular insight and mode of inhibition of α ‐Glucosidase and α ‐Amylase by Pahangensin A from Alpinia pahangensis Ridl.
by: Loo, Kong Yong, et al.
Published: (2019)

Curcumin, a bioactive compound of Turmeric (Curcuma longa) and its derivatives as α-amylase and α-glucosidase inhibitors
by: Roney, Miah, et al.
Published: (2024)

Protein denaturation in pilot scale spray dryer
by: Kioh, Shin Fang
Published: (2010)

Production of α-amylase by immobilized bacillus subtilis in polymeric polyHIPE matrix
by: Jimat, Dzun Noraini, et al.
Published: (2013)

Molecular Cloning and Expression of a Thermostable Α-Amylase from Geobacillus Sp.
by: Kassaye, Elias Kebede
Published: (2009)

Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002
by: Muhammad Suhaib, M. H., et al.
Published: (2013)

Denaturation behavior and conformational states of champedak galactose-binding lectin under various denaturing conditions / Nurul Iman Ahamed Kameel
by: Nurul Iman , Ahamed Kameel
Published: (2018)

Potential inhibitors of α-glucosidase and α-amylase enzymes from locally available fruit wastes by solid state fermentation
by: Ahmad Barkat, Anumsima, et al.
Published: (2019)

Characteristics of recombinant maltogenic amylase from Geobacillus sp. SK70
by: Sulong, Moohamad Ropaning, et al.
Published: (2017)

Electrochemical behaviour of denatured haemoglobin at the liquid|liquid interface
by: Herzog, G., et al.
Published: (2010)

Thermal Denaturation Kinetics of Whey Protein and Maltodextrin Mixture
by: Nuraini, Mohd Yusoff, et al.
Published: (2013)

Analytical quantification method of protein denaturation in spray drying
by: Suria, Mahmud
Published: (2012)

Synthesis And Molecular Docking Studies Of New Phenylisoxazole Quinoxaline-2-Amine Hybrids As Potential Α Amylase And Α-Glucosidase Inhibitors
by: Radzuan, Siti Nurshahira Mohd
Published: (2024)

Inhibitory Effects Of Edible Seaweeds, Polyphenolics and Alginates On The Activities Of Porcine Pancreatic α-amylase
by: Nazikussabah, Zaharudin, et al.
Published: (2017)

Expression and characterization of Geobacillus stearothermophilus SR74 recombinant a-Amylase in Pichia pastoris
by: Gandhi, Sivasangkary, et al.
Published: (2015)

Adsorption And Desorption Of Oxytetracyline Hydrochloride On The Activated Carbon
by: Nazir, Muhammad Ismathanif Md
Published: (2017)

Phenolic profiling and evaluation of in vitro antioxidant, α-glucosidase and α-amylase inhibitory activities of Lepisanthes fruticosa (Roxb) Leenh fruit extracts
by: Hasan Salahuddin, Mirfat Ahmad, et al.
Published: (2020)

Yield enhancement of recombinant α-Amylases in Bacillus amyloliquefaciens by ARTP mutagenesis-screening and medium optimization
by: Xu, Ting-Liang, et al.
Published: (2019)

Expression of thermostable α-amylase using formaldehyde dehydrogenase promoter in Meyerozyma guilliermondii strain SO
by: Mohamad Nasir, Nurul Syazwani
Published: (2018)

Synthesis and Heterocyclizations of 3,4-Dihydroquinazolin-2-yl Guanidine in the Search of New Anticancer Agents
by: Dolzhenko, Anton, et al.
Published: (2009)

Adsorption And Desorption Of Oxytetracycline Hydrochloride On Modified Activated Carbon
by: Zukfflay @ Zulkifli, Mohammad Hafeez
Published: (2017)

Improving the efficiency of new automatic dishwashing detergent formulation by addition of thermostable lipase, protease and amylase
by: Naganthran, Ashwini, et al.
Published: (2017)

Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger
by: I.Khalaf, Abu Bakir, et al.
Published: (2013)

Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger
by: Khalaf, Abubakir I., et al.
Published: (2014)