Bile salt deconjugation and cholesterol removal from media by Lactobacillus strains used as probiotics in chickens
BACKGROUND: Bile salt deconjugation by Lactobacillus strains is often closely linked to bile tolerance and survival of the strains in the gut and lowering of cholesterol in the host. The present study investigated the deconjugation of bile salts and removal of cholesterol by 12 Lactobacillus strains...
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| Format: | Article |
| Language: | English |
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Society of Chemical Industry
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13393/ http://psasir.upm.edu.my/id/eprint/13393/1/Bile%20salt%20deconjugation%20and%20cholesterol%20removal%20from%20media%20by%20Lactobacillus%20strains%20used%20as%20probiotics%20in%20chickens.pdf |
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| author | Ramasamy, Kalavathy Abdullah, Norhani Wong, Clemente Michael Vui Ling Chinna, Karuthan Ho, Yin Wan |
| author_facet | Ramasamy, Kalavathy Abdullah, Norhani Wong, Clemente Michael Vui Ling Chinna, Karuthan Ho, Yin Wan |
| author_sort | Ramasamy, Kalavathy |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | BACKGROUND: Bile salt deconjugation by Lactobacillus strains is often closely linked to bile tolerance and survival of the strains in the gut and lowering of cholesterol in the host. The present study investigated the deconjugation of bile salts and removal of cholesterol by 12 Lactobacillus strains in vitro. The 12 strains were previously isolated from the gastrointestinal tract of chickens.
RESULTS: The 12 Lactobacillus strains could deconjugate sodium glycocholate (GCA, 16.87-100%) and sodium taurocholate (TCA, 1.69-57.43%) bile salts to varying degrees, with all strains except L. salivarius I 24 having a higher affinity for GCA. The 12 Lactobacillus strains also showed significant (P < 0.05) differences in their ability to remove cholesterol from the growth medium (26.74-85.41%). Significant (P < 0.05) correlations were observed between cholesterol removal and deconjugation of TCA (r = 0.83) among the L. reuteri strains (C1, C10 and C16) and between cholesterol removal and deconjugation of TCA (r = 0.38) and GCA (r = 0.70) among the L. brevis strains (I 12, I 23, I 25, I 211 and I 218). In contrast, although L. gallinarum I 16 and I 26 and L. panis C 17 showed high deconjugating activity, there was no correlation between cholesterol removal and deconjugation of bile salts in these strains.
CONCLUSION: The results showed that the 12 Lactobacillus strains were able to deconjugate bile salts and remove cholesterol in vitro, but not all strains with high deconjugating activity removed cholesterol effectively. |
| first_indexed | 2025-11-15T07:53:46Z |
| format | Article |
| id | upm-13393 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:53:46Z |
| publishDate | 2010 |
| publisher | Society of Chemical Industry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-133932019-11-27T06:48:14Z http://psasir.upm.edu.my/id/eprint/13393/ Bile salt deconjugation and cholesterol removal from media by Lactobacillus strains used as probiotics in chickens Ramasamy, Kalavathy Abdullah, Norhani Wong, Clemente Michael Vui Ling Chinna, Karuthan Ho, Yin Wan BACKGROUND: Bile salt deconjugation by Lactobacillus strains is often closely linked to bile tolerance and survival of the strains in the gut and lowering of cholesterol in the host. The present study investigated the deconjugation of bile salts and removal of cholesterol by 12 Lactobacillus strains in vitro. The 12 strains were previously isolated from the gastrointestinal tract of chickens. RESULTS: The 12 Lactobacillus strains could deconjugate sodium glycocholate (GCA, 16.87-100%) and sodium taurocholate (TCA, 1.69-57.43%) bile salts to varying degrees, with all strains except L. salivarius I 24 having a higher affinity for GCA. The 12 Lactobacillus strains also showed significant (P < 0.05) differences in their ability to remove cholesterol from the growth medium (26.74-85.41%). Significant (P < 0.05) correlations were observed between cholesterol removal and deconjugation of TCA (r = 0.83) among the L. reuteri strains (C1, C10 and C16) and between cholesterol removal and deconjugation of TCA (r = 0.38) and GCA (r = 0.70) among the L. brevis strains (I 12, I 23, I 25, I 211 and I 218). In contrast, although L. gallinarum I 16 and I 26 and L. panis C 17 showed high deconjugating activity, there was no correlation between cholesterol removal and deconjugation of bile salts in these strains. CONCLUSION: The results showed that the 12 Lactobacillus strains were able to deconjugate bile salts and remove cholesterol in vitro, but not all strains with high deconjugating activity removed cholesterol effectively. Society of Chemical Industry 2010-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13393/1/Bile%20salt%20deconjugation%20and%20cholesterol%20removal%20from%20media%20by%20Lactobacillus%20strains%20used%20as%20probiotics%20in%20chickens.pdf Ramasamy, Kalavathy and Abdullah, Norhani and Wong, Clemente Michael Vui Ling and Chinna, Karuthan and Ho, Yin Wan (2010) Bile salt deconjugation and cholesterol removal from media by Lactobacillus strains used as probiotics in chickens. Journal of the Science of Food and Agriculture, 90 (1). pp. 65-69. ISSN 0022-5142; ESSN: 1097-0010 https://onlinelibrary.wiley.com/doi/full/10.1002/jsfa.3780 10.1002/jsfa.3780 |
| spellingShingle | Ramasamy, Kalavathy Abdullah, Norhani Wong, Clemente Michael Vui Ling Chinna, Karuthan Ho, Yin Wan Bile salt deconjugation and cholesterol removal from media by Lactobacillus strains used as probiotics in chickens |
| title | Bile salt deconjugation and cholesterol removal from media by Lactobacillus strains used as probiotics in chickens |
| title_full | Bile salt deconjugation and cholesterol removal from media by Lactobacillus strains used as probiotics in chickens |
| title_fullStr | Bile salt deconjugation and cholesterol removal from media by Lactobacillus strains used as probiotics in chickens |
| title_full_unstemmed | Bile salt deconjugation and cholesterol removal from media by Lactobacillus strains used as probiotics in chickens |
| title_short | Bile salt deconjugation and cholesterol removal from media by Lactobacillus strains used as probiotics in chickens |
| title_sort | bile salt deconjugation and cholesterol removal from media by lactobacillus strains used as probiotics in chickens |
| url | http://psasir.upm.edu.my/id/eprint/13393/ http://psasir.upm.edu.my/id/eprint/13393/ http://psasir.upm.edu.my/id/eprint/13393/ http://psasir.upm.edu.my/id/eprint/13393/1/Bile%20salt%20deconjugation%20and%20cholesterol%20removal%20from%20media%20by%20Lactobacillus%20strains%20used%20as%20probiotics%20in%20chickens.pdf |