Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model
The objective of this study was to regenerate the tracheal epithelium using autologous nasal respiratory epithelial cells in a sheep model. Respiratory epithelium and fibroblast cells were harvested from nasal turbinates and cultured for 1 week. After confluence, respiratory epithelium and fibroblas...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
S. Karger
2010
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/13337/ http://psasir.upm.edu.my/id/eprint/13337/1/Autologous%20implantation%20of%20bilayered%20tissue.pdf |
| _version_ | 1848842085934825472 |
|---|---|
| author | Mohd Yunus, Mohd Heikal Saim, Aminuddin Jahendran, Jeevanan Chen, Hui Cheng Syed Hussain, Sharifah Salmah Idrus, Ruszymah |
| author_facet | Mohd Yunus, Mohd Heikal Saim, Aminuddin Jahendran, Jeevanan Chen, Hui Cheng Syed Hussain, Sharifah Salmah Idrus, Ruszymah |
| author_sort | Mohd Yunus, Mohd Heikal |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The objective of this study was to regenerate the tracheal epithelium using autologous nasal respiratory epithelial cells in a sheep model. Respiratory epithelium and fibroblast cells were harvested from nasal turbinates and cultured for 1 week. After confluence, respiratory epithelium and fibroblast cells were suspended in autologous fibrin polymerized separately to form a tissue-engineered respiratory epithelial construct (TEREC). A 3 × 2 cm2 tracheal mucosal defect was created, and implanted with TEREC and titanium mesh as a temporary scaffold. The control groups were divided into 2 groups: polymerized autologous fibrin devoid of cells (group 1), and no construct implanted (group 2). All sheep were euthanized at 4 weeks of implantation. Gross observation of the trachea showed minimal luminal stenosis formation in the experimental group compared to the control groups. Macroscopic evaluation revealed significant mucosal fibrosis in control group 1 (71.8%) as compared to the experimental group (7%). Hematoxylin and eosin staining revealed the presence of minimal overgrowth of fibrous connective tissue covered by respiratory epithelium. A positive red fluorescence staining of PKH26 on engineered tissue 4 weeks after implantation confirmed the presence of cultured nasal respiratory epithelial cells intercalated with native tracheal epithelial cells. Scanning electron microscopy showed the presence of short microvilli representing immature cilia on the surface of the epithelium. Our study showed that TEREC was a good replacement for a tracheal mucosal defect and was able to promote natural regenesis of the tracheal epithelium with minimal fibrosis. This study highlighted a new technique in the treatment of tracheal stenosis. |
| first_indexed | 2025-11-15T07:53:32Z |
| format | Article |
| id | upm-13337 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:53:32Z |
| publishDate | 2010 |
| publisher | S. Karger |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-133372019-10-21T01:57:16Z http://psasir.upm.edu.my/id/eprint/13337/ Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model Mohd Yunus, Mohd Heikal Saim, Aminuddin Jahendran, Jeevanan Chen, Hui Cheng Syed Hussain, Sharifah Salmah Idrus, Ruszymah The objective of this study was to regenerate the tracheal epithelium using autologous nasal respiratory epithelial cells in a sheep model. Respiratory epithelium and fibroblast cells were harvested from nasal turbinates and cultured for 1 week. After confluence, respiratory epithelium and fibroblast cells were suspended in autologous fibrin polymerized separately to form a tissue-engineered respiratory epithelial construct (TEREC). A 3 × 2 cm2 tracheal mucosal defect was created, and implanted with TEREC and titanium mesh as a temporary scaffold. The control groups were divided into 2 groups: polymerized autologous fibrin devoid of cells (group 1), and no construct implanted (group 2). All sheep were euthanized at 4 weeks of implantation. Gross observation of the trachea showed minimal luminal stenosis formation in the experimental group compared to the control groups. Macroscopic evaluation revealed significant mucosal fibrosis in control group 1 (71.8%) as compared to the experimental group (7%). Hematoxylin and eosin staining revealed the presence of minimal overgrowth of fibrous connective tissue covered by respiratory epithelium. A positive red fluorescence staining of PKH26 on engineered tissue 4 weeks after implantation confirmed the presence of cultured nasal respiratory epithelial cells intercalated with native tracheal epithelial cells. Scanning electron microscopy showed the presence of short microvilli representing immature cilia on the surface of the epithelium. Our study showed that TEREC was a good replacement for a tracheal mucosal defect and was able to promote natural regenesis of the tracheal epithelium with minimal fibrosis. This study highlighted a new technique in the treatment of tracheal stenosis. S. Karger 2010-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13337/1/Autologous%20implantation%20of%20bilayered%20tissue.pdf Mohd Yunus, Mohd Heikal and Saim, Aminuddin and Jahendran, Jeevanan and Chen, Hui Cheng and Syed Hussain, Sharifah Salmah and Idrus, Ruszymah (2010) Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model. Cells Tissues Organs, 192 (5). pp. 292-302. ISSN 1422-6405; ESSN: 1422-6421 10.1159/000318675 |
| spellingShingle | Mohd Yunus, Mohd Heikal Saim, Aminuddin Jahendran, Jeevanan Chen, Hui Cheng Syed Hussain, Sharifah Salmah Idrus, Ruszymah Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model |
| title | Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model |
| title_full | Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model |
| title_fullStr | Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model |
| title_full_unstemmed | Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model |
| title_short | Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model |
| title_sort | autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model |
| url | http://psasir.upm.edu.my/id/eprint/13337/ http://psasir.upm.edu.my/id/eprint/13337/ http://psasir.upm.edu.my/id/eprint/13337/1/Autologous%20implantation%20of%20bilayered%20tissue.pdf |